Product Sheet CG1196
Description
BACKGROUND ErbB4 is a member of the epidermal growth factor receptor (EGFR, ErbB) family that mediates responses to neuregulins and other EGF-like growth factors. It is a central regulator of cardiovascular and neural development as well as differentiation of the mammary gland. A role for ErbB4 has also been implicated in malignancies and heart diseases. Although expression of the ErbB4 receptor tyrosine kinase in breast cancer is generally regarded as a marker for favorable patient prognosis, controversial exceptions have been reported. Alternative splicing of ErbB4 pre-mRNAs results in the expression of distinct receptor isoforms with differential susceptibility to enzymatic cleavage and different downstream signaling protein recruitment potential that could affect tumor progression in different ways. Four structurally and functionally distinct ErbB4 isoforms have recently been identified.1
ErbB4 is activated by binding of growth factors in the EGF family, which are encoded by at least nine genes. The ligand-activated receptors can signal either through homodimerization or through heterodimerization with other EGFR family members. Each receptor/ligand combination has the potential to recruit and activate a unique set of interacting proteins, thereby initiating signaling cascades which culminate in distinct cellular responses.2 Autophosphorylation of Tyr1056 in ErbB4 may provide a docking site for downstream signaling components and is critical for coupling ErbB4 to prostate tumor suppression.3
ErbB4 is activated by binding of growth factors in the EGF family, which are encoded by at least nine genes. The ligand-activated receptors can signal either through homodimerization or through heterodimerization with other EGFR family members. Each receptor/ligand combination has the potential to recruit and activate a unique set of interacting proteins, thereby initiating signaling cascades which culminate in distinct cellular responses.2 Autophosphorylation of Tyr1056 in ErbB4 may provide a docking site for downstream signaling components and is critical for coupling ErbB4 to prostate tumor suppression.3
REFERENCES
1. Muraoka-Cook, RS et al.: J Mammary Gland Biol Neoplasia.13:235-46,2008
2. Kaushansky, A et al.: Chem. Biol. 15: 808-817, 2008
3. Gallo, RM et al.: Biochem. Biophy. Res Commun. 349: 372-382, 2006
2. Kaushansky, A et al.: Chem. Biol. 15: 808-817, 2008
3. Gallo, RM et al.: Biochem. Biophy. Res Commun. 349: 372-382, 2006
Products are for research use only. They are not intended for human, animal, or diagnostic applications.
(Click to Enlarge)
Top: Immunohistochemical staining of human stomach shows moderate cytoplasmic positivity in glandular cells. Bottom: Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli and cytoplasm.
Details
Cat.No.: | CG1196 |
Antigen: | Receptor tyrosine-protein kinase erbB-4 Precursor recombinant protein epitope signature tag (PrEST) |
Isotype: | Rabbit IgG |
Species & predicted species cross- reactivity ( ): | Human, Rat |
Applications & Suggested starting dilutions:* | WB 1:100-1:500 IP n/d IHC 1:50-1:200 ICC n/d FACS n/d |
Predicted Molecular Weight of protein: | 29 kDa |
Specificity/Sensitivity: | Detects endogenous ErbB4 proteins without cross-reactivity with other family members. |
Storage: | Store at -20°C, 4°C for frequent use. Avoid repeated freeze-thaw cycles. |
*Optimal working dilutions must be determined by end user.
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Rabbit Erb4 Antibody: Rabbit Erb4 Antibody | Size: 100 ul | CAT.#: CG1196 | Price: $461.00 |