Product Sheet CA1104
Description
BACKGROUND FOXL2 is a member of the forkhead/HNF-3-related gene family of transcription factors that are highly conserved and involved in many developmental processes and in cellular differentiation. As the other member of the family, FOXL2 possesses a well-conserved winged helix domain that binds DNA to a 7-bp core recognition motif (5\'-G/A-T/C-C/A-A-A-C/T-A-3\'). Another feature of FOXL2 is the presence of a polyalanine domain of 14 alanines, located to the C-terminus with respect to the DNA binding domain.1 Although the physiological function of this polyalanine tract remains unknown, its expansion from 14 to 24 residues accounts for 30% of the reported mutations in BPES patients. The 14 to 24 expansion induces extensive nuclear and cytoplasmic FOXL2 protein aggregation. FOXL2 is a critical factor essential for ovary differentiation and maintenance, and repression of the genetic program for somatic testis determination.2 FOXL2 prevents trans-differentiation of ovary to testis through transcriptional repression of the Sertoli cell-promoting gene SOX9. FOXL2 has apoptotic activity in ovarian cells. Moreover, FOXL2 suppresses ESR1-mediated transcription of PTGS2/COX2 stimulated by tamoxifen. FOXL2 is also a regulator of CYP19 expression. In addition, FOXL2 participates in SMAD3-dependent transcription of FST via the intronic SMAD-binding element. It was shown that FoxL2 plays a key role in activin induction of the FSH-beta gene.3 It is a transcriptional repressor of STAR. And it was shown that FOXL2 activates SIRT1 transcription under cellular stress conditions. It also activates transcription of OSR2. Mutations in FOXL2 gene are a cause of blepharophimosis syndrome and premature ovarian failure. Moreover, it was demonstrated that Mutant FOXL2 is a potential driver in the pathogenesis of adult-type granulosa-cell tumors (GCTs), the most common type of malignant ovarian sex cord–stromal tumor (SCST).4
REFERENCES
REFERENCES
1. Carlsson, P. & Mahlapuu, M.: Dev. Biol. 250:1-23, 2002
2. Ottolenghi, C. et al: Hum. Mol. Genet. 14:2053-62, 2005
3. Corpuz, P.S. et al: Mol. Endocrinol. 24:1037-51, 2010
4. Shah, S.P. et al: New England J. Med. 360:2719-29, 2009
2. Ottolenghi, C. et al: Hum. Mol. Genet. 14:2053-62, 2005
3. Corpuz, P.S. et al: Mol. Endocrinol. 24:1037-51, 2010
4. Shah, S.P. et al: New England J. Med. 360:2719-29, 2009
Products are for research use only. They are not intended for human, animal, or diagnostic applications.
(Click to Enlarge) Western Blot detection of FOXL2 proteins in various cell lysates using FOXL2 Antibody.
Details
Cat.No.: | CA1104 |
Antigen: | Short peptide from human FOXL2 sequence. |
Isotype: | Rabbit IgG |
Species & predicted species cross- reactivity ( ): | Human, Mouse, Rat |
Applications & Suggested starting dilutions:* | WB 1:1000 IP n/d IHC 1:50 - 1:200 ICC n/d FACS n/d |
Predicted Molecular Weight of protein: | 39 kDa |
Specificity/Sensitivity: | Detects endogenous levels of FOXL2 proteins without cross-reactivity with other related proteins. |
Storage: | Store at -20°C, 4°C for frequent use. Avoid repeated freeze-thaw cycles. |
*Optimal working dilutions must be determined by end user.