Product Sheet CA1338
Description
BACKGROUND GAPDH (Glyceraldehyde 3-phosphate dehydrogenase, G3PDH) is an enzyme that catalyzes the reversible Oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD), the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules.1 In addition to this long established metabolic function, GAPDH has recently been implicated in several non-metabolic processes, including transcription activation, initiation of apoptosis, and ER to Golgi vesicle shuttling.2 The enzyme exists as a tetramer of identical chains.
Because the GAPDH gene is often stably and constitutively expressed at high levels in most tissues and cells, it is considered a housekeeping gene. For this reason, GAPDH is commonly used by biological researchers as a loading control for western blot and as a control for RT-PCR. However, many researchers report different regulation of GAPDH under specific conditions.3 Therefore, the use of GAPDH as loading control has to be controlled carefully.
Because the GAPDH gene is often stably and constitutively expressed at high levels in most tissues and cells, it is considered a housekeeping gene. For this reason, GAPDH is commonly used by biological researchers as a loading control for western blot and as a control for RT-PCR. However, many researchers report different regulation of GAPDH under specific conditions.3 Therefore, the use of GAPDH as loading control has to be controlled carefully.
REFERENCES
1. Voet, D. & Voet, J. G.: Biochemistry, Third Edition. J. Wiley & Sons, Hoboken, NJ, 2004
2. Tarze, A. et al: Oncogene 26:2606-20, 2007
3. Graven, K.K. et al: J. Biol. Chem. 269:24446-53, 1994
2. Tarze, A. et al: Oncogene 26:2606-20, 2007
3. Graven, K.K. et al: J. Biol. Chem. 269:24446-53, 1994
Products are for research use only. They are not intended for human, animal, or diagnostic applications.
(Click to Enlarge) Western Blot detection of GAPDH proteins in rat heart tissue (A), rat brain tissue (B), rat live tissue (C), rat skeletal muscle tissue (D), MM453 whole cell (E), MM231 whole cell (F), smmc whole cell (G), HeLa whole cell (H), and HT1080 whole cell (I) lysates using GAPDH Antibody.
Details
Cat.No.: | CA1338 |
Antigen: | Short peptide from human GAPDH sequence. |
Isotype: | Rabbit IgG |
Species & predicted species cross- reactivity ( ): | Human, Mouse, Rat |
Applications & Suggested starting dilutions:* | WB 1:1000 IP n/d IHC 1:50 - 1:200 ICC n/d FACS n/d |
Predicted Molecular Weight of protein: | 35 kDa |
Specificity/Sensitivity: | Detects endogenous levels of GAPDH proteins without cross-reactivity with other related proteins. |
Storage: | Store at -20°C, 4°C for frequent use. Avoid repeated freeze-thaw cycles. |
*Optimal working dilutions must be determined by end user.
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Rabbit GAPDH Antibody: Rabbit GAPDH Antibody | Size: 100 ul | CAT.#: CA1338 | Price: $302.00 |