Product Sheet CG1483
Description
BACKGROUND The family of Protein Arginine N-Methyltransferases (PRMTs) catalyze the sequential transfer of a methyl group from AdoMet to the side chain nitrogens of arginine residues within proteins to form methylated arginine derivatives and S-adenosyl-L-homocysteine.1 There are eleven different PRMT genes (PRMT1-11) whose biological function remains under explored. With regard to the dimethylation product, PRMTs are distinguished into type I enzymes, which catalyze the asymmetric NG,NG-dimethyl-arginine, and the type II subfamily, which consists of PRMT5, PRMT7, and PRMT9 and generates symmetric NG,NG′-dimethylation. PRMT2 was isolated based on its sequence similarity with PRMT1. So far no methyltransferase activity has been revealed for PRMT2.2 PRMTs regulate various cellular processes such as DNA repair and transcription, RNA processing, signal transduction, and nucleo-cytoplasmic localization. Like histone lysine methylation, methylation of histone arginine residues can either induce or inhibit transcription depending on the residue being modified and the type of methylation being introduced.3
REFERENCES
1. Litt M et al.: Biosci Rep. 29:131-41, 2009.
2. Meyer R et al.: J Steroid Biochem Mol Biol. 107:1-14, 2007.
3. Lee YH. & Stallcup MR: Mol Endocrinol. 23:425-33, 2009.
2. Meyer R et al.: J Steroid Biochem Mol Biol. 107:1-14, 2007.
3. Lee YH. & Stallcup MR: Mol Endocrinol. 23:425-33, 2009.
Products are for research use only. They are not intended for human, animal, or diagnostic applications.
(Click to Enlarge) Cell extracts were separated on SDS-PAGE and blotted with Anti-PRMT1 at 1 μg/mL and developed using Goat Anti-Rabbit IgG-Peroxidase and a chemiluminescent substrate. (Left Lane) MCF7 cells; (Middle Lane) Chinese ovary cells; (Right Lane) HEK 293-T cells.
Details
Cat.No.: | CG1483 |
Antigen: | Synthetic peptide corresponding to amino acids 309-324 of rat PRMT1 conjugated to KLH via an N-terminal added cysteine residue. This sequence is conserved in human and rat. |
Isotype: | Rabbit IgG |
Species & predicted species cross- reactivity ( ): | Hamster, Human, Rat, Mouse, Monkey |
Applications & Suggested starting dilutions:* | WB 1-2 ug/mL IP 2.5-5 ug IHC n/d ICC n/d FACS n/d |
Predicted Molecular Weight of protein: | 42 kDa |
Specificity/Sensitivity: | Detects endogenous PRMT1 proteins without cross-reactivity with other family members. |
Storage: | Store at -20°C, 4°C for frequent use. Avoid repeated freeze-thaw cycles. |
*Optimal working dilutions must be determined by end user.
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Rabbit Protein Arginine N-Methyltransferase Antibody: Rabbit Protein Arginine N-Methyltransferase Antibody | Size: 100 ul | CAT.#: CG1483 | Price: $384.00 |