Description
BACKGROUND Foxp3 is a 48 kDa protein consisting of 431-amino acid residues and a member of the forkhead/winged-helix family of transcriptional regulators. It contains four potential functional domains including the repressor, ZF, LZ and fork-head (FKH) domains. The repressor domain is localized to the N-terminal region of FOXP3 and is required to repress NFAT-mediated transcriptional activity. The FKH domain is critical for both DNA binding and nuclear localization.1
Foxp3 locus is transcribed in the regulatory T cell. For outside of the lymphoid system, Foxp3 expression was found in breast epithelial cells, lung respiratory epithelial cells, and prostate epithelial cells, although not in liver, heart, and intestine.2 In human, Foxp3 is expressed in normal human breast epithelial cells but is silenced in most human breast cancers.
Foxp3 functions as the master regulator in the development and function of regulatory T (Treg) cells.3 Nevertheless, there is still a controversy as to whether defect Treg alone explains the lethal autoimmune diseases observed in the mutant mouse and human. Moreover, Foxp3 as the first X-linked tumor suppressor directly targets two critical oncogenes HER2/ErbB2 and SKP2 and represses their transcription activity to inhibit cell growth in breast cancer.4
REFERENCES
1. Merkel, M. Et al: J. Biol. Chem. 277:7405-11, 2001
2. Hamilton, M.T. et al: Am J Physiol Endocrinol Metab 275: E1016-E1022, 1998
3. Kamei, Y. et al: Biochem. Int. 26:923-34, 1992
2. Hamilton, M.T. et al: Am J Physiol Endocrinol Metab 275: E1016-E1022, 1998
3. Kamei, Y. et al: Biochem. Int. 26:923-34, 1992
Products are for research use only. They are not intended for human, animal, or diagnostic applications.
Top: Western Blot detection of Foxp3 proteins in 293 cell lysate containing overexpressed human Foxp3 proteins using Foxp3 Antibody. Middle: This antibody stains paraffin-embedded human lymph node tissue in immunohistochemical analysis.
Bottom: It also stains PANC-1 cells in confocal immunofluorescent testing (Foxp3 Antibody: Green; Actin filaments: Red; DRAQ5 DNA dye: Blue).
Details
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Cat.No.:
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CP10109
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Antigen:
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Purified recombinant human Foxp3 fragments expressed in E. coli.
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Isotype:
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Mouse IgG
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Species & predicted
species cross-
reactivity ( ):
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Human
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Applications &
Suggested starting
dilutions:*
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WB 1:1000
IP n/d
IHC 1:200
ICC 1:200
FACS n/d
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Predicted Molecular
Weight of protein:
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48 kDa
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Specificity/Sensitivity:
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Detects Foxp3 proteins without cross-reactivity with other family members.
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Storage:
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Store at -20°C, 4°C for frequent use. Avoid repeated freeze-thaw cycles.
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*Optimal working dilutions must be determined by end user.