Cytofect™ Transfection Reagents are exceptionally gentle, and have minimal effects on cellular morphology as seen in these post-transfection images of various primary cells and cell lines. Post-transfection cell viability is a key parameter of transfection system performance. The Cytofect™ Transfection Reagents provide exceptionally high post-transfection viabilities compared to both electroporation and other chemical based transfection reagents. Even after passaging, cells transfected with Cytofect™ continue to express transgenic protein as seen below.
Image: Cytofect™ Transfection Kits maintain cell morphology & transgene expression even after subculturing.
Here, Human Umbilical Vein Endothelial Cells (HUVEC) are transfected with a GFP expression plasmid.
Examples of High Post-Transfection Cell Health, Viability and Morphology
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Examples of High Cell Viability (Low Toxicity) Post-Transfection using Cytofect™ Transfection Kits:
| Cytofect™ | Cell Type |
Efficiency | Viability |
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| Cell Line | Human Breast Adenocarcinoma (MCF-7) | 40-90% | 80-95% |
| Human Cervical Epithelial Adenocarcinoma (HeLa) | |||
| Human Embryonic Kidney (293) | |||
| Chinese Hamster Ovary (CHO) | |||
| Chondrocyte | Human Chondrocytes (HC) | 35% | 80-95% |
| Endothelial | Human Aortic Endothelial Cells (HAOEC) | 40-85% | 80-95% |
| Human Coronary Artery Endothelial Cells (HCAEC) | |||
| Human Dermal Lymphatic Microvascular Endothelial Cells (HDLMVEC) | |||
| Human Dermal Microvascular Endothelial Cells (HDMVEC) | |||
| Human Lung Microvascular Endothelial Cells (HLMVEC) | |||
| Human Pulmonary Artery Endothelial Cells (HPAEC) | |||
| Human Umbilical Vein Endothelial Cells (HUVEC) | |||
| Bovine Aortic Endothelial Cells (BAOEC) | |||
| Bovine Coronary Artery Endothelial Cells (BCAEC) | |||
| Bovine Pulmonary Artery Endothelial Cells (BPAEC) | |||
| Porcine Pulmonary Artery Endothelial Cells (PPAEC) | |||
| Rat Aortic Endothelial Cells (RAOEC) | |||
| Rat Brain Microvascular Endothelial Cells (RBMVEC) | |||
| Epithelial | Human Bronchial Epithelial Cells (HBEpC) | 40-70% | 70-95% |
| Human Epidermal Keratinocytes (HEK) | |||
| Human Mammary Epithelial Cells (HMEpC) | |||
| Human Prostate Epithelial Cells (HPrEpC) | |||
| Human Tracheal Epithelial Cells (HTEpC) | |||
| Human Umbilical Epithelial Cells | |||
| Fibroblast | Human Cardiac Fibroblasts (HCF) | 30-70% | 90-95% |
| Human Dermal Fibroblasts (HDF) | |||
| Human Lung Fibroblasts (HLF) | |||
| Hepatocyte | Rat Hepatocytes (RH) | 40-60% | 70-95% |
| HUVEC | Human Umbilical Vein Endothelial Cells (HUVEC) | 40-85% | 80-95% |
| Neuron | Rat Cortical Neurons (RCoN) | 10-20% | 80-95% |
| Preadipocyte | Human Preadipocytes (HPAd) | 25% | 80-95% |
| Skeletal Muscle | Human Skeletal Muscle Cells (HSkMC) | 15-30% | 80-95% |
| Smooth Muscle | Human Aortic Smooth Muscle Cells (HAOSMC) | 35-65% | 80-95% |
| Human Brachiocephalic Artery Smooth Muscle Cells (HBcASMC) | |||
| Human Carotid Artery Smooth Muscle Cells (HCtASMC) | |||
| Human Coronary Artery Smooth Muscle Cells (HCASMC) | |||
| Human Internal Thoracic Artery Smooth Muscle Cells (HITASMC) | |||
| Human Pulmonary Artery Smooth Muscle Cells (HPASMC) | |||
| Human Subclavian Artery Smooth Muscle Cells (HScASMC) | |||
| Human Umbilical Vein Smooth Muscle Cells (HUVSMC) |