5 Important Cell Culture Rules
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Instructions HNSC

Description

Human Neural Stem Cells (HNSC) are self-renewing, generated throughout an adult's life via neurogenesis.  These multipotent adult stem cells generate the main phenotype of the nervous system, differentiating into neurons, astrocytes, and oligodendrocytes.
HNSC play important roles for development, learning and hippocampal plasticity.  They are also used to study age-related declines in proliferation, as well as neurological diseases like stroke, multiple sclerosis, and Parkinson's disease.  The cells respond to injury, and can be differentiated to replace lost or injured neurons.  They migrate in a directed fashion to brain tumors and help replace dying neurons in injured adult brain tissue.
Cell Applications HNSC are primary cells derived from the cortex region of human brain (single donor).  They are cryopreserved at first passage.  Our HNSC stain positive for β-tubulin III, GFAP and oligodendrocyte marker O4 when cultured in Human Neural Differentiation Medium for 10 days.  Each lot is tested for its ability to form neurospheres in Human Neural Stem Cell Growth Medium.
(Click to Enlarge) Human Neural Stem Cells (HNSC) and Derived Lineages.  HNSC differentiate to 3 lineages with the aid of specialized media. HNSC stained for Nestin (green) and Sox2 (red) (A). HNSC cultured in Medium 813D-100N differentiate to neurons (β III-Tubulin, green) (B), those in 813D-100A differentiate to astrocytes (GFAP, red) (C), and cells in Medium 813D-100O differentiate to oligodendrocytes (O4, green) (D). All cells are counterstained with nuclear-specific DAPI (blue).

Details

Tissue
Normal healthy human brain cortex
QC
No bacteria, yeast, fungi, mycoplasma, virus
Cryovial
2,000,000 HNSC (1st psg) cryopreserved in Freezing Med (040-50)
Kit
Cryovial frozen HNSC (HS820-f), Growth Medium (813-250), Neural Stem Cell Dissociation Solution (076-20), 10cm non-TC dish x2
Proliferating
N/A
Doublings
N/A
Applications
Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
Instructions HNSC

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Products

Product Size CAT.# Price Quantity
Cryopreserved HNSC Total Kit: 2x10^6 Cells (Fetal), Medium & Culture Dishes (See Details tab for specifics) Size: 1 Kit CAT.#: HS820K-20f Price: $890.89
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Cryopreserved HNSC, fetal: Frozen HNSC (2x10^6) Size: 1 Cryovial CAT.#: HS820-20f Price: $785.66
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Proliferating HNSC, fetal: Actively growing, dividing cells, in medium Size: T-25 Flask CAT.#: HS821-25f Price: $659.00
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Proliferating HNSC, fetal: Actively growing, dividing cells, in medium Size: T-75 Flask CAT.#: HS821-75f Price: $849.00
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Proliferating HNSC, fetal: Actively growing, dividing cells, in medium Size: 6 Well CAT.#: HS821-6Wf Price: $849.00
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Proliferating HNSC, fetal: Actively growing, dividing cells, in medium Size: 96 Well CAT.#: HS821-96Wf Price: $969.00
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Proliferating HNSC, fetal: Actively growing, dividing cells, in medium Size: 24 Well CAT.#: HS821-24Wf Price: $899.00
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Related Products

Product Size CAT.# Price Quantity
Human Neural Stem Cell Basal Medium: Basal medium (contains no growth supplement).  Add GS before use. Size: 250 ml CAT.#: 812-250 Price: $39.82
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Human Neural Stem Cell Differentiation Medium: General HNSC Differentiation Medium Size: 250 ml CAT.#: 813D-250 Price: $128.13
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Human Neural Stem Cell Differentiation Medium: Differentiates HNSC to Astrocytes Size: 100 ml CAT.#: 813D-100A Price: $61.50
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Human Neural Stem Cell Differentiation Medium: Differentiates HNSC to Neurons Size: 100 ml CAT.#: 813D-100N Price: $73.80
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Human Neural Stem Cell Differentiation Medium: Differentiates HNSC to Oligodendrocytes Size: 100 ml CAT.#: 813D-100O Price: $61.50
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Human Neural Stem Cell Growth Medium: All-in-one ready-to-use Size: 250 ml CAT.#: 813-250 Price: $116.24
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Human Neural Stem Cell Growth Medium Kit: Basal medium & growth supplement sold together packaged separately Size: yields 250 ml CAT.#: 813K-250 Price: $128.07
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Human Neural Stem Cell Growth Supplement: Added to Basal Medium to create Growth Medium Size: 7 ml (2 parts) CAT.#: 813-GS Price: $88.25
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Extended Family Products

Product Size CAT.# Price Quantity
Anti-GDNF: Rabbit Glial Derived Neurotrophic Factor Antibody Size: 100 ul CAT.#: CA1465 Price: $302.38
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Anti-NCAM1: Rabbit Neural Cell Adhesion Molecule Antibody Size: 100 ul CAT.#: CA1055 Price: $302.38
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Anti-Nestin: Rabbit Nestin Antibody Size: 100 ul CAT.#: CY1123 Price: $384.38
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Anti-NSE: Rabbit Neuron Specific Enolase Antibody Size: 100 ul CAT.#: CA1061 Price: $302.38
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CNTF: Human Ciliary Neurotrophic Factor Size: 20 ug CAT.#: RP1059-20 Price: $193.73
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CNTF: Human Ciliary Neurotrophic Factor Size: 100 ug CAT.#: RP1059-100 Price: $484.31
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CNTF: Human Ciliary Neurotrophic Factor Size: 1000 ug CAT.#: RP1059-1000 Price: $3,174.94
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CNTF, Animal-Free: Human Ciliary Neurotrophic Factor, Animal-Free Size: 20 ug CAT.#: RP1059AF-20 Price: $213.10
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CNTF, Animal-Free: Human Ciliary Neurotrophic Factor, Animal-Free Size: 100 ug CAT.#: RP1059AF-100 Price: $532.74
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CNTF, Animal-Free: Human Ciliary Neurotrophic Factor, Animal-Free Size: 1000 ug CAT.#: RP1059AF-1000 Price: $3,492.43
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Cyto-X Colorimetric Cell Counting Reagent: 500 tests Size: 1 Bottle CAT.#: 028-01 Price: $138.84
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Cyto-X Colorimetric Cell Counting Reagent: 100 tests Size: Sample CAT.#: 028-S Price: $35.88
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Cytofect™ Neuron Transfection Kit: 100 x 24-Well Rxns Size: 1 Kit CAT.#: TF886K Price: $403.59
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GDNF: Human Glial Cell-Derived Neurotrophic Factor Size: 10 ug CAT.#: RP1002-10 Price: $193.73
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GDNF: Human Glial Cell-Derived Neurotrophic Factor Size: 100 ug CAT.#: RP1002-100 Price: $624.23
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GDNF: Human Glial Cell-Derived Neurotrophic Factor Size: 1000 ug CAT.#: RP1002-1000 Price: $5,327.44
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GDNF ELISA Kit, human: Human Glial Derived Neurotrphic Factor ELISA Kit Size: 96 Wells CAT.#: CL0362 Price: $581.18
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GDNF, Animal-Free: Human Glial Cell-Derived Neurotrophic Factor, Animal-Free Size: 10 ug CAT.#: RP1002AF-10 Price: $213.10
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GDNF, Animal-Free: Human Glial Cell-Derived Neurotrophic Factor, Animal-Free Size: 1000 ug CAT.#: RP1002AF-1000 Price: $5,860.18
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GDNF, Animal-Free: Human Glial Cell-Derived Neurotrophic Factor, Animal-Free Size: 100 ug CAT.#: RP1002AF-100 Price: $686.65
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Resources/Documents

5 Important Cell Culture Rules

Format: PDF

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Cell Apps Flyer Nervous System

Format: PDF

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Cell Apps Flyer Brain Cells

Format: PDF

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Cell Apps Poster Primary Cells

Format: PDF

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Cell Applications Inc Brochure

Format: PDF

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