5 Important Cell Culture Rules
MSDS Cryopreserved Cells
Cell Apps Flyer Nervous System
Cell Apps Flyer Brain Cells
Cell Apps Poster Primary Cells
Cell Applications Inc Brochure
Instructions HNSC
Description
Human Neural Stem Cells (HNSC) are self-renewing, generated throughout an adult's life via neurogenesis. These multipotent adult stem cells generate the main phenotype of the nervous system, differentiating into neurons, astrocytes, and oligodendrocytes.
HNSC play important roles for development, learning and hippocampal plasticity. They are also used to study age-related declines in proliferation, as well as neurological diseases like stroke, multiple sclerosis, and Parkinson's disease. The cells respond to injury, and can be differentiated to replace lost or injured neurons. They migrate in a directed fashion to brain tumors and help replace dying neurons in injured adult brain tissue.
Cell Applications HNSC-3D are primary cells derived from the cortex region of human brain (single donor). They are cryopreserved at first passage as neurospheres with limited propagation in this format.
Primary HNSC are also available as adherent cultures (HNSC-2D), which are proliferative and multipotent.
(Click to Enlarge) Human Neural Stem Cells (HNSC-3D) and Derived Lineages. HNSC differentiate to 3 lineages with the aid of specialized media. HNSC stained for Nestin (green) and Sox2 (red) (A). HNSC cultured in Medium 813D-100N differentiate to neurons (β III-Tubulin, green) (B), those in 813D-100A differentiate to astrocytes (GFAP, red) (C), and cells in Medium 813D-100O differentiate to oligodendrocytes (O4, green) (D). All cells are counterstained with nuclear-specific DAPI (blue).
Details
Tissue | Normal healthy human brain cortex |
QC | No bacteria, yeast, fungi, mycoplasma, virus |
Cryovial | 2,000,000 HNSC-3D (1st psg) cryopreserved in Freezing Med (040-50) |
Kit | Cryovial frozen HNSC (HS820-20f), Growth Medium (813-250), Neural Stem Cell Dissociation Solution (076-20), 10cm non-TC dish x2 |
Proliferating | N/A |
Doublings | N/A |
Applications | Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use. |
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Cryopreserved Human Neural Stem Cell (HNSC-3D, suspension) Total Kit, fetal: 2x10^6 Cells (Fetal), Medium & Culture Dishes (See Details tab for specifics) | Size: 1 Kit | CAT.#: HS820K-20f | Price: $997.00 | |
| Cryopreserved Human Neural Stem Cell (HNSC-3D, suspension), fetal: Frozen HNSC (2x10^6) | Size: 1 Cryovial | CAT.#: HS820-20f | Price: $828.00 | |
| Proliferating Neural Stem Cells (HNSC-3D, suspension), fetal: Cell culture in medium | Size: 96 Well | CAT.#: HS821-96Wf | Price: $1,138.00 | |
| Proliferating Neural Stem Cells (HNSC-3D, suspension), fetal: Cell culture in medium | Size: T-25 Flask | CAT.#: HS821-25f | Price: $828.00 | |
| Proliferating Neural Stem Cells (HNSC-3D, suspension), fetal: Cell culture in medium | Size: T-75 Flask | CAT.#: HS821-75f | Price: $1,018.00 | |
| Proliferating Neural Stem Cells (HNSC-3D, suspension), fetal: Cell culture in medium | Size: 24 Well | CAT.#: HS821-24Wf | Price: $1,018.00 |
Related Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| HNSC Growth Medium: All-in-one ready-to-use | Size: 250 ml | CAT.#: 813-250 | Price: $140.00 | |
| HNSC Growth Medium Kit: Basal medium & growth supplement sold together packaged separately. Combine to make complete growth medium. | Size: yields 250 ml | CAT.#: 813K-250 | Price: $152.00 | |
| HNSC Basal Medium: Basal medium (contains no growth supplement). Add GS before use. | Size: 250 ml | CAT.#: 812-250 | Price: $58.00 | |
| HNSC Growth Supplement: Added to Basal Medium to create Growth Medium | Size: 7 ml (2 parts) | CAT.#: 813-GS | Price: $118.00 | |
| HNSC Differentiation Medium, Astrocyte Lineage: Differentiates HNSC to Astrocytes. Differentiation also requires NSC Differentiation Coating Solutions A & B. | Size: 100 ml | CAT.#: 813D-100A | Price: $73.00 | |
| HNSC Differentiation Medium, Neuron Lineage: Differentiates HNSC to Neurons. Differentiation also requires NSC Differentiation Coating Solutions A & B. | Size: 100 ml | CAT.#: 813D-100N | Price: $88.00 | |
| HNSC Differentiation Medium, Oligodendrocyte Lineage: Differentiates HNSC to Oligodendrocytes. Differentiation also requires NSC Differentiation Coating Solutions A & B. | Size: 100 ml | CAT.#: 813D-100O | Price: $73.00 | |
| Neural Stem Cell Differentiation Coating Solution A: Coating Solution A used specifically for the differentiation of neural stem cells, such as HNSC and i-HNSC. | Size: 10 ml | CAT.#: 034-10 | Price: $58.00 | |
| Neural Stem Cell Differentiation Coating Solution B: Coating Solution B used specifically for the differentiation of neural stem cells, such as HNSC and i-HNSC. | Size: 10 ml | CAT.#: 035-10 | Price: $58.00 | |
| HNSC Differentiation Medium: General HNSC Differentiation Medium. Differentiation also requires NSC Differentiation Coating Solutions A & B. | Size: 250 ml | CAT.#: 813D-250 | Price: $152.00 |
Extended Family Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Rabbit GDNF Antibody: Rabbit Glial Derived Neurotrophic Factor Antibody | Size: 100 ul | CAT.#: CA1465 | Price: $302.00 | |
| Rabbit Neuron Specific Enolase Antibody: Rabbit Neuron Specific Enolase Antibody | Size: 100 ul | CAT.#: CA1061 | Price: $375.00 | |
| Human Ciliary Neurotrophic Factor (CNTF): Human Ciliary Neurotrophic Factor | Size: 20 ug | CAT.#: RP1059-20 | Price: $194.00 | |
| Human Ciliary Neurotrophic Factor (CNTF): Human Ciliary Neurotrophic Factor | Size: 100 ug | CAT.#: RP1059-100 | Price: $484.00 | |
| Human Ciliary Neurotrophic Factor (CNTF): Human Ciliary Neurotrophic Factor | Size: 1000 ug | CAT.#: RP1059-1000 | Price: $3,175.00 | |
| Cytofect Neuron Transfection Kit (100 x 24-Wells): 100 x 24-Well Rxns | Size: 1 Kit | CAT.#: TF886K | Price: $512.00 | |
| Human Glial Derived Neurotrophic Factor (GDNF): Human Glial Cell-Derived Neurotrophic Factor | Size: 10 ug | CAT.#: RP1002-10 | Price: $194.00 | |
| Human Glial Derived Neurotrophic Factor (GDNF): Human Glial Cell-Derived Neurotrophic Factor | Size: 100 ug | CAT.#: RP1002-100 | Price: $624.00 | |
| Human Glial Derived Neurotrophic Factor (GDNF): Human Glial Cell-Derived Neurotrophic Factor | Size: 1000 ug | CAT.#: RP1002-1000 | Price: $5,327.00 | |
| Human GDNF ELISA Kit: Human Glial Derived Neurotrphic Factor ELISA Kit | Size: 96 Wells | CAT.#: CL0362 | Price: $581.00 | |
| Human GDNF, Animal-Free: Human Glial Cell-Derived Neurotrophic Factor, Animal-Free | Size: 100 ug | CAT.#: RP1002AF-100 | Price: $687.00 | |
| Human GDNF, Animal-Free: Human Glial Cell-Derived Neurotrophic Factor, Animal-Free | Size: 1000 ug | CAT.#: RP1002AF-1000 | Price: $5,860.00 | |
| Human GDNF, Animal-Free: Human Glial Cell-Derived Neurotrophic Factor, Animal-Free | Size: 10 ug | CAT.#: RP1002AF-10 | Price: $213.00 | |
| Human CNTF, Animal-Free: Human Ciliary Neurotrophic Factor, Animal-Free | Size: 20 ug | CAT.#: RP1059AF-20 | Price: $213.00 | |
| Human CNTF, Animal-Free: Human Ciliary Neurotrophic Factor, Animal-Free | Size: 100 ug | CAT.#: RP1059AF-100 | Price: $533.00 | |
| Human CNTF, Animal-Free: Human Ciliary Neurotrophic Factor, Animal-Free | Size: 1000 ug | CAT.#: RP1059AF-1000 | Price: $3,492.00 |
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