Product Sheet CL0573
Description
BACKGROUND CD40L (also called CD154) is a member of the tumor necrosis factor family of ligands, plays a major role in immune responses via its receptor, CD40.1 CD40L is mainly expressed on activated CD4+ T lymphocytes. Recently, CD40L has been detected on the surfaces of activated platelets and shown to activate endothelium. It was also shown that HUVEC cell express CD40L.2 Whereas CD40, a member of the tumor necrosis factor receptor (TNFR) family, has been found on the surface of B lymphocytes, monocytes, hematopoietic progenitors, dendritic cells (DCs), endothelial cells, epithelial cells and so on. CD40L-CD40 interaction plays important role in control of thymus-dependent humoral immunity and cell mediated immune responses. CD40L is the most crucial molecule for activating antigen-presenting cells (APCs) and thereby initiating the immune response.3 Deletion or mutations of the CD40L molecule leads to severe immunodeficiency, in both man and mouse, characterized by hypogamma-globulinemia as well as T cell defects in cell mediated immunity.4 Moreover, blockade of the CD40L/CD40 pathway by antibodies to CD40L has been used, in experimental animals,5 to effectively prevent and treat a variety of autoimmune diseases as well as allograft rejection without inducing generalized immune suppression.
REFERENCES
1. Daoussis D et al.: Clin. Diagn. Lab. Immunol. 11:635-641, 2004.
2. Mendez-Cruz AR et al.: Immunol. Lett. 111:116-123, 2007.
3. Boisvert J et al.: J. Immunol. 173: 3647-3652, 2004.
4. Weller S et al.: Proc. Natl. Acad. Sci. USA 98:1166-1170, 2001.
5. Lutgens E et al.: Proc. Natl. Acad. Sci. USA 97:7564-7469, 2000.
2. Mendez-Cruz AR et al.: Immunol. Lett. 111:116-123, 2007.
3. Boisvert J et al.: J. Immunol. 173: 3647-3652, 2004.
4. Weller S et al.: Proc. Natl. Acad. Sci. USA 98:1166-1170, 2001.
5. Lutgens E et al.: Proc. Natl. Acad. Sci. USA 97:7564-7469, 2000.
Products are for research use only. They are not intended for human, animal, or diagnostic applications.
(Click to Enlarge) Cluster of Differentiation 4 Ligand (CD40L) Standard Curve: Using the Human CD40L ELISA Kit, O.D. data was graphed against CD40L protein concentration. The TMB reaction was incubated at 37°C for 18 min.
Details
Cat.No.: | CL0573 |
Target Protein Species: | Human |
Range: | 62.5pg/ml – 4000 pg/ml |
Specificity: | No detectable cross-reactivity with other cytokines |
Storage: | Store at 4°C. Use within 6 months. |
ELISA Kits are based on standard sandwich enzyme-linked immunosorbent assay technology. Freshly prepared standards, samples, and solutions are recommended for best results.
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Human CD40L ELISA Kit: Human Cluster of Differentiation 40 Ligand ELISA Kit | Size: 96 Wells | CAT.#: CL0573 | Price: $533.00 |