Product Sheet CL0326
Description
BACKGROUND EGF is the founding member of the EGF-family of protein. Members of this protein family have highly similar structural and functional characteristics. Human EGF is a 6045-Da protein with 53 amino acid residues and three intramolecular disulfide bonds. Besides EGF itself other family members include: HB-EGF, TGF-alpha, Amphiregulin, Epiregulin, Epigen, Betacellulin, NRG-1, -2, -3, and -4. EGF has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide. EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture.1
EGF acts by binding with high affinity to epidermal growth factor receptor (EGFR) on the cell surface and stimulating the intrinsic protein-tyrosine kinase activity of the receptor. The tyrosine kinase activity, in turn, initiates a signal transduction cascade that results in a variety of biochemical changes within the cell, a rise in intracellular calcium levels, increased glycolysis and protein synthesis, and increases in the expression of certain genes including the gene for EGFR - that ultimately lead to DNA synthesis and cell proliferation.2
EGF acts by binding with high affinity to epidermal growth factor receptor (EGFR) on the cell surface and stimulating the intrinsic protein-tyrosine kinase activity of the receptor. The tyrosine kinase activity, in turn, initiates a signal transduction cascade that results in a variety of biochemical changes within the cell, a rise in intracellular calcium levels, increased glycolysis and protein synthesis, and increases in the expression of certain genes including the gene for EGFR - that ultimately lead to DNA synthesis and cell proliferation.2
REFERENCES
1. Carpenter, G. & Cohen, S.:J. Biol. Chem. 265:7709-12, 1990
2. Eblin, K.: Toxicol. Sci.109:169-71, 2009
2. Eblin, K.: Toxicol. Sci.109:169-71, 2009
Products are for research use only. They are not intended for human, animal, or diagnostic applications.
(Click to Enlarge) Epidermal Growth Factor (EGF) Standard Curve: Using the Mouse EGF ELISA Kit, O.D. data was graphed against EGF protein concentration. The TMB reaction was incubated at 37°C for 11 min.
Details
Cat.No.: | CL0326 |
Target Protein Species: | Mouse |
Range: | 15.6pg/ml – 1000pg/ml |
Specificity: | No detectable cross-reactivity with other cytokines |
Storage: | Store at 4°C. Use within 6 months. |
ELISA Kits are based on standard sandwich enzyme-linked immunosorbent assay technology. Freshly prepared standards, samples, and solutions are recommended for best results.
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Mouse EGF ELISA Kit: Mouse Epidermal Growth Factor ELISA Kit | Size: 96 Wells | CAT.#: CL0326 | Price: $511.00 |