Product Sheet CP10296
Description
BACKGROUND CDC25 is a dual specificity phosphatase with three isoforms in mammalian cells - CDC25A, B and C. CDC25 activates Cdk complexes that control progression through the cell cycle. CDC25 is also involved in the DNA damage checkpoints. Regulation of CDC25 occurs through a variety of mechanisms including phosphorylation, interaction with 14.3.3 proteins, subcellular localization and protein degradation.1 CDC25B is phosphorylated and activated by aurora kinase A at the start of mitosis, and as mitosis progresses is then further phosphorylated in an auto-amplification loop, along with CDC25C, by the CDK1/cyclin B complex. Cdc25C is also phosphorylated during mitosis by PLK1, leading to greater activity of the CDK1/cyclin B complex. Similarly, CDC25A acts during the G1/S phase of the cell cycle in concert with the CDK2/cyclin E complex. Both CDC25A and CDC25B can be activated by the DNA damage checkpoint kinase, Chk1 and thereby inhibit mitosis.2
CDC25C is highly conserved during evolution and it plays a key role in the regulation of cell division. It is a tyrosine phosphatase and directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis.3 It is also thought to suppress p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described.
CDC25C is highly conserved during evolution and it plays a key role in the regulation of cell division. It is a tyrosine phosphatase and directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis.3 It is also thought to suppress p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described.
REFERENCES
1. Turowski,P. et al: Mol. Biol. Cell 14: 2984-98, 2003
2. Zwicker, J. et al: EMBO J. 14:4514-22, 1995
3. Mitra, J. & Schultz, R.M.: J. Cell Sci. 109:2407-2415, 1996
2. Zwicker, J. et al: EMBO J. 14:4514-22, 1995
3. Mitra, J. & Schultz, R.M.: J. Cell Sci. 109:2407-2415, 1996
Products are for research use only. They are not intended for human, animal, or diagnostic applications.
Western Blot detection of CDC25C proteins in various cell lysates using CDC25C Antibody.
Details
Cat.No.: | CP10296 |
Antigen: | Raised against recombinant human CDC25C fragments expressed in E. coli. |
Isotype: | Mouse IgG1 |
Species & predicted species cross- reactivity ( ): | Human, Mouse, Rat |
Applications & Suggested starting dilutions:* | WB 1:1000 IP n/d IHC n/d ICC n/d FACS n/d |
Predicted Molecular Weight of protein: | 60 kDa |
Specificity/Sensitivity: | Detects endogenous CDC25C proteins without cross-reactivity with other family members. |
Storage: | Store at -20°C, 4°C for frequent use. Avoid repeated freeze-thaw cycles. |
*Optimal working dilutions must be determined by end user.
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Mouse CDC25C Antibody: Mouse CDC25C Antibody | Size: 100 ul | CAT.#: CP10296 | Price: $333.00 |