Tissue RNA

Description

Total RNA prepared from normal human tissues using the modified guanidium thiocyanate method.  RNA is DNAse treated to remove contaminating genomic DNA and undergoes stringent quality control tests.  Purified RNA is ready for use in convenient 50 & 250 µg sizes for various downstream applications, including microarray hybridization, PCR cDNA synthesis and RT-PCR analysis.
 
See also Cell RNA
(Click to Enlarge) Tissue RNA.  Human Fetal Brain (lines 2, 3) and Human Adult Lung (lines 4, 5) Total RNA.  RNA was electrophoresed in 1% agarose gel to show the distinct 28S and 18S ribosomal RNA bands (lines 2, 4).  RT-PCR was performed with HSP60 primers to produce 2.2 kb product (lines 3, 5).

Details

Stringent quality control tests confirm RNA integrity and stability:

  • Distinct 28S and 18S ribosomal RNA were shown on 1% denaturing gel before and after 37C  incubation
  • No visible Genomic DNA band of 23 kb detected
  • No genomic DNA contamination detected following PCR amplification using house-keeping gene G3PDH primers
  • A 2.2 kb product from mRNA was detected following RT-PCR amplification using human heat shock gene Hsp60 primers
  • A discrete band of 2 kb was detected upon hybridization with a human beta-actin cDNA probe in Northern Blot analysis
  • Primers were designed to detect mRNA for genes of interest to confirm cell phenotype

Products

Product Size CAT.# Price Quantity
Human Brain RNA, Fetal: Total RNA prepared from human brain tissue, fetal Size: 50 ug CAT.#: 1F01-50 Price: $198.00
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Human Brain RNA, Fetal: Total RNA prepared from human brain tissue, fetal Size: 250 ug CAT.#: 1F01-250 Price: $595.00
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Human Heart RNA: Total RNA prepared from human heart tissue Size: 50 ug CAT.#: 1H30-50 Price: $158.00
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Human Heart RNA: Total RNA prepared from human heart tissue Size: 250 ug CAT.#: 1H30-250 Price: $595.00
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Human Small Intestine RNA: Total RNA prepared from human small intestine tissue Size: 50 ug CAT.#: 1H24-50 Price: $158.00
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Human Small Intestine RNA: Total RNA prepared from human small intestine tissue Size: 250 ug CAT.#: 1H24-250 Price: $595.00
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Human Kidney RNA: Total RNA prepared from human kidney tissue Size: 50 ug CAT.#: 1H50-50 Price: $158.00
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Human Kidney RNA: Total RNA prepared from human kidney tissue Size: 250 ug CAT.#: 1H50-250 Price: $595.00
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Human Liver RNA, Fetal : Total RNA prepared from human liver tissue, fetal Size: 50 ug CAT.#: 1F21-50 Price: $198.00
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Human Liver RNA, Fetal : Total RNA prepared from human liver tissue, fetal Size: 250 ug CAT.#: 1F21-250 Price: $595.00
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Human Liver RNA: Total RNA prepared from human liver tissue Size: 50 ug CAT.#: 1H21-50 Price: $158.00
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Human Liver RNA: Total RNA prepared from human liver tissue Size: 250 ug CAT.#: 1H21-250 Price: $595.00
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Human Lung RNA: Total RNA prepared from human lung tissue Size: 50 ug CAT.#: 1H40-50 Price: $158.00
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Human Lung RNA: Total RNA prepared from human lung tissue Size: 250 ug CAT.#: 1H40-250 Price: $595.00
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Human Pancreas RNA: Total RNA prepared from human pancreas tissue Size: 50 ug CAT.#: 1H23-50 Price: $158.00
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Human Pancreas RNA: Total RNA prepared from human pancreas tissue Size: 250 ug CAT.#: 1H23-250 Price: $595.00
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Human Placenta RNA: Total RNA prepared from human placental tissue Size: 50 ug CAT.#: 1H54-50 Price: $158.00
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Human Placenta RNA: Total RNA prepared from human placental tissue Size: 250 ug CAT.#: 1H54-250 Price: $595.00
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Human Prostate RNA: Total RNA prepared from human prostate tissue Size: 50 ug CAT.#: 1H52-50 Price: $158.00
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Human Prostate RNA: Total RNA prepared from human prostate tissue Size: 250 ug CAT.#: 1H52-250 Price: $595.00
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Human Skeletal Muscle RNA: Total RNA prepared from human skeletal muscle tissue Size: 50 ug CAT.#: 1H60-50 Price: $158.00
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Human Skeletal Muscle RNA: Total RNA prepared from human skeletal muscle tissue Size: 250 ug CAT.#: 1H60-250 Price: $595.00
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Human Spleen RNA: Total RNA prepared from human spleen tissue Size: 50 ug CAT.#: 1H70-50 Price: $158.00
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Human Spleen RNA: Total RNA prepared from human spleen tissue Size: 250 ug CAT.#: 1H70-250 Price: $595.00
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Human Stomach RNA: Total RNA prepared from human stomach tissue Size: 50 ug CAT.#: 1H20-50 Price: $158.00
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Human Stomach RNA: Total RNA prepared from human stomach tissue Size: 250 ug CAT.#: 1H20-250 Price: $595.00
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Human Testes RNA: Total RNA prepared from human testes tissue Size: 50 ug CAT.#: 1H51-50 Price: $158.00
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Human Testes RNA: Total RNA prepared from human testes tissue Size: 250 ug CAT.#: 1H51-250 Price: $595.00
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Human Lung RNA, Fetal : Total RNA prepared from human lung tissue Size: 50 ug CAT.#: 1F40-50 Price: $158.00
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Human Lung RNA, Fetal : Total RNA prepared from human lung tissue Size: 250 ug CAT.#: 1F40-250 Price: $595.00
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Human Skeletal Muscle RNA, Fetal: Total RNA prepared from human skeletal muscle tissue, fetal Size: 50 ug CAT.#: 1F60-50 Price: $198.00
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Human Skeletal Muscle RNA, Fetal: Total RNA prepared from human skeletal muscle tissue, fetal Size: 250 ug CAT.#: 1F60-250 Price: $740.00
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Related Products

Product Size CAT.# Price Quantity
Custom RNA Services: Total RNA from Human Tissues Size: 1 Project CAT.#: CSTSVC-RHT Price: $0.00

Publications

2017
Mitsuyoshi, H., K. Yasui, T. Hara, H. Taketani, H. Ishiba, A. Okajima, Y. Seko, A. Umemura, T. Nishkawa, K. Yamaguchi m. Moriguchi, M. Minami and Y. itoh. 2017. Hepatic nucleotide binding oligomerization  domain-like receptors  pyrin domain-containing 3 inflammasomes are associated with the histologic severity of nonalcoholic fatty liver disease. Wiley, doi: 10.1111/hepr.12883.
2016
Aburatani, H., S. Tsutsumi, K. Nighimura, H. Sakumoto and S. Kawai. 2016. Anti-CLDN6 antibody. Patent US 9274119 B2.
Nair, T., P. Kommareddi, M. Galano, D. Miller, B. Kakaraparthi, S. Telian, H. Arts, H. El-Kashlan, A. Kilijanczyk, A. Lassig, M. Graham, S. Fisher, S. Stoll R. Nair, J. Elder and T. Cary. 2016. SLC44A2 single nucleotide polymorphisms, isoforms, and expression: Association with severity of Meniere's disease? Genomics, 108:201-208.
2015
Yoshida, K., H. Aburatani and S. Ishikawa. 2015. ANTI-DLL3 ANTIBODY. Patent Application US 20150368355 A1.
2014
Eikmans, M., J. Anholts, and F. Claas. 2014. Gene Expression Analysis by qPCR in Clinical Kidney Transplantation. Methods in Molec Biol, 1160:147-163.
Uranbileg, B., K. Enooku, Y. Soroida, R. Ohkawa, Y. Kudo, H. Nakagawa, R. Tateishi, H. Yoshida, S. Shinzawa, K. Moriya, N. Ohtomo, T. Nishikawa, Y. Inoue, T. Tomiya, S. Kojima, T. Matsuura, K. Koike, Y. Yatomi and H. Iked. 2014. High ubiquitous mitochondrial creatine kinase expression in hepatocellular carcinoma denotes a poor prognosis with highly malignant potential. Intl J Cancer, 134:2189-2198.
2012
Aburatani, H., S. Ishikawa, and S. Kawai. 2012. Diagnosis and treatment of cancer using anti-itm2a antibody. Patent Application US 20140193420 A1.
Al Menhali, A. 2012. Parathyroid Hormone-Like Hormone (PTHLH): A Novel Parietal Cell Growth Factor Regulated by Gastrin. PhD Dissertation, U Michigan.
Hosogi, S., H. Miyazaki, K. Nakajima, E. Ashihara, N. Niisato, K. Kusuzaki and Y. Marunaka. 2012. An Inhibitor of Na+/H+ Exchanger (NHE), Ethyl-Isopropyl Amiloride (EIPA), Diminishes Proliferation of MKN28 Human Gastric Cancer Cells by Decreasing the Cytosolic Cl- Concentration via DIDS-Sensitive Pathways. Cellular Physiol & Biochem, 30:1241-1253.
Mok, P., S. Cheong, C. Leong, K. Chua, and O. Ainoon. 2012. Extended and stable gene expression via nucleofection of MIDGE construct into adult human marrow mesenchymal stromal cells. Cytotechnology, 64:203-216.
Soroida, Y., R. Ohkawa, H. Nakagawa, Y. Satoh, H. Yoshida, H. Kinoshita, R. Tateishi, R. Masuzaki, K. Enooku, S. Shiina, T. Sato, S. Obi, T. Hoshino, R. Nagatomo, S. Okubo, H. Yokota, K. Koike, Y. Yatomi, and H. Ikeda. 2012. Increased activity of serum mitochondrial isoenzyme of creatine kinase in hepatocellular carcinoma patients predominantly with recurrence. J Hepatology, 57:330-336.
Tanabe, Y. Y. Fujiware, A. Matsuzaki, E. Fujita, T. Kasahara, S. Yuasa, and T. Momoi. 2012. Temporal expression and mitochondrial localization of a Foxp2 isoform lacking the forkhead domain in developing Purkinje cells. J Neurochm, 122:72-80.  
2011
Yoshida, K., H. Aburatani, and S. Ishikawa. 2011. Anti-dll3 antibody. Patent Application US 20120328624 A1.
2010
Ohsawa, R., H. Miyazaki, N. Niisato, A. Shiozaki, Y. Iwasaki, E. Otsuji and Y. Marunaka. 2010. Intracellular chloride regulates cell proliferation through the activation of stress-activated protein kinases in MKN28 human gastric cancer cells. J Cell Physiol, 223:764-770.
Sogawa, C., C. Mitsuhata, K. Kumagai-Morioka, N. Sogawa, K. Ohyama, K. Morita, K. Kozai, T. Dohi, and S. Kitayama. 2010. Expression and Function of Variants of Human Catecholamine Transporters Lacking the Fifth Transmembrane Region Encoded by Exon 6. PLOS One, DOI: 10.1371/journal.pone.0011945.
2009
Aburatani, H., S. Ishikawa, and S. Kawai. 2009. Diagnosis and treatment of cancer using anti-tm4sf20 antibody. Patent Application US 20120004117 A1.
Aburatani, H., S. Tsutsumi, K. Nishimura, H. Sakumoto, and S. Kawai. 2009. Anti-cldn6 antibody. Patent US 20110059469 A1.