Instructions HRPTEpC
MSDS Cryopreserved Cells
Cell Apps Flyer Epithelial Cells
5 Important Cell Culture Rules
Cell Apps Poster Primary Cells
Cell Applications Inc Brochure
Description
Human Renal Proximal Tubular Epithelial Cells (HRPTEpC) provide a useful tool for studying various aspects of pathology and biology of human kidney cells in vitro. HRPTEpC from Cell Applications, Inc. have recently been used in a study of metabolic enzymes responding to energy depletion in renal cells, and they a pivotal role in kidney function including reabsorbtion of glucose and protein, and also play a part in the glucotoxicity associated with diabetes.
Characterization: Morphology consistent with epithelial origin, and positive for epithelial cell marker cytokeratin 18
Details
Tissue | Normal healthy human Kidney |
QC | No bacteria, yeast, fungi, mycoplasma, virus |
Bioassay | Attach, spread, proliferate in Growth Med |
Cryovial | 500,000 HRPTEpC (1st or 2nd psg) frozen in Basal Med w/ 10% FBS, 10% DMSO |
Kit | Cryovial frozen HRPTEpC (930-05a), Growth Medium (911-500), Subcltr Rgnt Kit (090K) |
Proliferating | Shipped in Tsfr Med, psg 3, flasks or plates |
Doublings | At least 6 |
Applications | Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use. |
Products
Product | Size | CAT.# | Price | Quantity |
---|---|---|---|---|
Cryopreserved Human Renal Proximal Tubular Epithelial Cells Total Kit, adult: 5x10^5 Cells (Adult), Medium & Subculture Reagents (See Details tab for specifics) | Size: 1 Kit | CAT.#: 930K-05a | Price: $911.00 | |
Cryopreserved Renal Proximal Tubular Epithelial Cells (HRPTEpC), adult: Frozen HRPTEpC (5x10^5) | Size: 1 Cryovial | CAT.#: 930-05a | Price: $715.00 | |
Proliferating Renal Proximal Tubular Epithelial Cells (HRPTEpC), adult: Actively growing, dividing cells in medium | Size: T-25 Flask | CAT.#: 931-25a | Price: $715.00 | |
Proliferating Renal Proximal Tubular Epithelial Cells (HRPTEpC), adult: Actively growing, dividing cells in medium | Size: T-75 Flask | CAT.#: 931-75a | Price: $905.00 | |
Proliferating Renal Proximal Tubular Epithelial Cells (HRPTEpC), adult: Actively growing, dividing cells in medium | Size: 96 Well | CAT.#: 931-96Wa | Price: $1,025.00 | |
Cryopreserved Human Renal Proximal Tubular Epithelial Cells Total Kit, fetal: 5x10^5 Cells (Fetal), Medium & Subculture Reagents (See Details tab for specifics) | Size: 1 Kit | CAT.#: 930K-05f | Price: $911.00 | |
Cryopreserved Renal Proximal Tubular Epithelial Cells (HRPTEpC), fetal: Frozen HRPTEpC (5x10^5) | Size: 1 Cryovial | CAT.#: 930-05f | Price: $715.00 | |
Proliferating Renal Proximal Tubular Epithelial Cells (HRPTEpC), fetal: Actively growing, dividing cells in medium | Size: T-25 Flask | CAT.#: 931-25f | Price: $715.00 | |
Proliferating Renal Proximal Tubular Epithelial Cells (HRPTEpC), fetal: Actively growing, dividing cells in medium | Size: T-75 Flask | CAT.#: 931-75f | Price: $905.00 | |
Proliferating Renal Proximal Tubular Epithelial Cells (HRPTEpC), fetal: Actively growing, dividing cells in medium | Size: 96 Well | CAT.#: 931-96Wf | Price: $1,025.00 | |
Cryopreserved Human Renal Proximal Tubular Epithelial Cells Total Kit, neonatal: 5x10^5 Cells (Neonatal), Medium & Subculture Reagents (See Details tab for specifics) | Size: 1 Kit | CAT.#: 930K-05n | Price: $911.00 | |
Cryopreserved Renal Proximal Tubular Epithelial Cells (HRPTEpC), neonatal: Frozen HRPTEpC (5x10^5) | Size: 1 Cryovial | CAT.#: 930-05n | Price: $715.00 | |
Proliferating Renal Proximal Tubular Epithelial Cells (HRPTEpC), neonatal: Actively growing, dividing cells in medium | Size: T-25 Flask | CAT.#: 931-25n | Price: $715.00 | |
Proliferating Renal Proximal Tubular Epithelial Cells (HRPTEpC), neonatal: Actively growing, dividing cells in medium | Size: T-75 Flask | CAT.#: 931-75n | Price: $905.00 | |
Proliferating Renal Proximal Tubular Epithelial Cells (HRPTEpC), neonatal: Actively growing, dividing cells in medium | Size: 96 Well | CAT.#: 931-96Wn | Price: $1,025.00 |
Related Products
Product | Size | CAT.# | Price | Quantity |
---|---|---|---|---|
Human RenaEpi Basal Medium: Basal medium (contains no growth supplement). Add GS before use. | Size: 500 ml | CAT.#: 910-500 | Price: $85.00 | |
Human RenaEpi Growth Medium: All-in-one ready-to-use | Size: 500 ml | CAT.#: 911-500 | Price: $143.00 | |
Human RenaEpi Growth Medium Kit: Basal medium & growth supplement sold together packaged separately | Size: Yields 500 ml | CAT.#: 911K-500 | Price: $155.00 | |
Human RenaEpi Growth Supplement: Added to Basal Medium to create Growth Medium | Size: 10 ml | CAT.#: 911-GS | Price: $91.00 |
Extended Family Products
Product | Size | CAT.# | Price | Quantity |
---|---|---|---|---|
Monoclonal IL-1 Receptor Antagonist Antibody: Monoclonal IL-1 Receptor Antagonist Antibody | Size: 100 ul | CAT.#: CB19846 | Price: $302.00 | |
Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO. | Size: 50 ml | CAT.#: 040-50 | Price: $54.00 | |
Cytofect Epithelial Cell Transfection Kit (124 x 24-Wells): 124 x 24-Well Rxns | Size: 1 Kit | CAT.#: TF102K | Price: $465.00 | |
Cytofect Epithelial Cell Transfection Sample Kit (25 x 24-Wells): 25 x 24-Well Rxns | Size: 1 Sample Kit | CAT.#: TF102KS | Price: $62.00 | |
Renal Proximal Tubule Epithelial Cell RNA (HRPTEpC RNA), Adult: Total RNA prepared from Human Renal Proximal Tubular Epithelial Cells, adult | Size: 10 ug | CAT.#: 930-R10a | Price: $514.00 | |
Renal Proximal Tubule Epithelial Cell RNA (HRPTEpC RNA), Adult: Total RNA prepared from Human Renal Proximal Tubular Epithelial Cells, adult | Size: 25 ug | CAT.#: 930-R25a | Price: $1,028.00 | |
Renal Proximal Tubule Epithelial Cell RNA (HRPTEpC RNA), Fetal: Human Renal Proximal Tubular Epithelial Cells RNA, fetal | Size: 10 ug | CAT.#: 930-R10f | Price: $465.00 | |
Renal Proximal Tubule Epithelial Cell RNA (HRPTEpC RNA), Fetal: Total RNA prepared from Human Renal Proximal Tubular Epithelial Cells, fetal | Size: 25 ug | CAT.#: 930-R25f | Price: $930.00 | |
Human IL-1 alpha ELISA Kit: Human Interleukin-1 alpha ELISA Kit | Size: 96 wells | CAT.#: CL0389 | Price: $484.00 | |
Human Interleukin-1-Alpha (IL-1 alpha): Human Interleukin-1-alpha | Size: 10 ug | CAT.#: RP1173-10 | Price: $194.00 | |
Human Interleukin-1-Alpha (IL-1 alpha): Human Interleukin-1-alpha | Size: 100 ug | CAT.#: RP1173-100 | Price: $624.00 | |
Human Interleukin-1-Alpha (IL-1 alpha): Human Interleukin-1-alpha | Size: 1000 ug | CAT.#: RP1173-1000 | Price: $5,327.00 | |
Human IL-1 beta ELISA Kit: Human Interleukin-1 beta ELISA Kit | Size: 96 wells | CAT.#: CL0392 | Price: $587.00 | |
Human Kidney RNA: Total RNA prepared from human kidney tissue | Size: 50 ug | CAT.#: 1H50-50 | Price: $158.00 | |
Human Kidney RNA: Total RNA prepared from human kidney tissue | Size: 250 ug | CAT.#: 1H50-250 | Price: $595.00 | |
Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution | Size: 1 Kit | CAT.#: 090K | Price: $63.00 |
Resources/Documents
Citations
Publications
2017
Bidwell, G., F. Mahdi, Q. Shao, O. Logue, J. Waller, C. Reese and A. Chade. 2017. A kidney-selective biopolymer for targeted drug delivery. Am J Physiol Renal Physiol, 312: F54-F64.
2016
Ito, H., O. Ichiyanagi, S. Naito, V. Bilim, Y. Tomita, Y. Kato, A. Nagaoka and N. Tsuchiya. 2016. GSK-3 directly regulates phospho-4EBP1 in renal cell carcinoma cell-line: an intrinsic subcellular mechanism for resistance to mTORC1 inhibition. BMC Cancer 16:393.
Zhou, X., R. Liu, S. Duan, G. Huang, Y. Ye and Y. Kong. 2016. High Glucose Enhances oxLDL-Induced Apoptosis in Human Renal Proximal Tubular Epithelial Cells Largely via Inducing Lectin-Like ox-LDL Receptor-1. Pharmacology, 98:20-28.
2014
Jackson, E., D. Gillespie, Z. Mi, D. Cheng, R. Bansal, K. Janesko-Feldman and P. Kochanek. 2014.Role of 2′,3′-cyclic nucleotide 3′-phosphodiesterase in the renal 2′,3′-cAMP-adenosine pathway. Am J Physiol – Renal Physiol, 307:F14-F24.
2012
Jackson, E. and D. Gillespie. 2012. Extracellular 2′,3′-cAMP and 3′,5′-cAMP stimulate proliferation of preglomerular vascular endothelial cells and renal epithelial cells. Am J Physiol – Renal Physiol, 303:F954-F962.