Product Sheet RP1007

Description

BACKGROUND Endocrine-gland-derived vascular endothelial growth factor, or EG-VEGF, is angiogenic growth factor specifically expressed in the ovaries, testis, adrenal and placental tissues. The identification of tissue-selective angiogenic factors raises the possibility that other secreted molecules in this class exist. EG-VEGF expression correlates with vascularity in polycystic ovary syndrome, a leading cause of infertility.

Recombinant human EG-VEGF is a non-glycosylated protein containing 86 amino acids and having a molecular mass of 9.6 kDa.

Products are for research use only. They are not intended for human, animal, or diagnostic applications.

Details

Cat. No.:
RP1007
 
Alternative Name:
Prokineticin 1, PROK1
 
Source:
E. coli
 
Physical Appearance:
Sterile Filtered white lyophilized (freeze-dried) powder.
 
Formulation:
Recombinant human EG-VEGF is lyophilized with no additives
 
Stability:
Lyophilized product is very stable at -20°C. Reconstituted material should be aliquoted and frozen at -20°C. It is recommended that a carrier protein (0.1% HSA or BSA) is added for long term storage.
 
Reconstitution:
Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.
 
Protein Content and Purity determined by:
UV spectroscopy at 280 nm.
RP-HPLC calibrated against a known standard.
Quantitation against a known standard via reducing and non-reducing SDS-PAGE gels.
 
Endotoxin Level:
Endotoxin level, as measured by LAL analysis, is <0.01ng/ug or <0.1EU/ug.
 
Biological Activity:
The activity is determined by the dose-dependent proliferation of MIA PaCa-2 cells and is typically 1-4 ug/mL.
 
AA Sequence:
AVITGACERD VQCGAGTCCA ISLWLRGLRM CTPLGREGEE CHPGSHKVPF FRKRKHHTCP CLPNLLCSRF PDGRYRCSMD LKNINF
 

Products

Product Size CAT.# Price Quantity
Human Endocrine Gland Vacular Enothelial Growth Factor (EG-VEGF): Human Endocrine Gland Vascular Enothelial Growth Factor Size: 20 ug CAT.#: RP1007-20 Price: $194.00
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Human Endocrine Gland Vacular Enothelial Growth Factor (EG-VEGF): Human Endocrine Gland Vascular Enothelial Growth Factor Size: 100 ug CAT.#: RP1007-100 Price: $484.00
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Human Endocrine Gland Vacular Enothelial Growth Factor (EG-VEGF): Human Endocrine Gland Vascular Enothelial Growth Factor Size: 1000 ug CAT.#: RP1007-1000 Price: $3,175.00
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Resources/Documents