Product Sheet CB13678
Description
BACKGROUND CD31, a member of the immunoglobulin superfamily, is a 130 kDa transmembrane glycoprotein also designated as PECAM-1 (platelet endothelial cell adhesion molecule1). CD-31 is normally found on endothelial cells (EC), platelets, macrophages and Kupffer cells, granulocytes, T / NK cells, lymphocytes, megakaryocytes, fibroblasts, osteoclasts, neutrophils.1 It plays a major role in the adhesion cascade between EC and the inflammatory cells during inflammation in facilitating leucocyte migration and between EC during angiogenesis.2,3 It has recently been recognized for its angiogenic role. It is one of the best markers for benign and malignant vascular tumors, but also stains plasma cells, monocytes, and megakaryocytes.4
REFERENCES
1. Newman, P. J.. et al: Science 247: 1219, 1990.2. Kalinowska, A. & Losy, J. : Eur. J. Neurol. 13:1284, 2007.
3. Stockinger, H. et al. (1991). J. Immunol. 145:3889, 1991.
4. Tang, D. G. et al: J. Biol. Chem. 268:22883, 1993.
Products are for research use only. They are not intended for human, animal, or diagnostic applications.
(Click to Enlarge) Top: Specific detection of CD31/PECAM-1 proteins from HUVEC by Western Blot analysis using CD31/PECAM-1 mouse monclonal Antibody. Middle, upper: Immunohistochemical staining of human spleen capillary hemangioma tissue using CD31 mouse monoclonal antibody (left) or Anti-CD31 mouse monoclonal antibody pre-incubated with HUVEC lysates (right). Middle, lower: Human Coronary Artery Endothelial Cells (Cat#300-05a) stained using Anti-CD31 (1:100). Bottom: FACS analysis of HUVEC cells using CD31/PECAM-1 mouse monoclonal antibody versus control mouse IgG.
Details
Cat.No.: | CB13678 |
Antigen: | E. coli-expressed recombinant human CD31 cytoplasmic protein fragments. |
Isotype: | Mouse Monoclonal IgG1 |
Species & predicted species cross- reactivity ( ): | Detects endogenous levels of human CD31/PECAM-1 proteins. |
Applications & Suggested starting dilutions: | WB 1:1000 IP n/d IHC (Paraffin) n/d IF n/d FACS n/d |
Predicted Molecular Weight of protein: | 130 kDa |
Specificity/Sensitivity: | Does not cross-react with other endothelial cell proteins |
Storage: | Store at -20°C, 4°C for frequent use. Avoid repeated freeze-thaw cycles. |
*Optimal working dilutions must be determined by end user.
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Monoclonal CD31 Antibody: Monoclonal CD31 Antibody | Size: 100 ul | CAT.#: CB13678 | Price: $302.00 |
Resources/Documents
Publications
2012
Ramírez-Sáncheza, I., P. Mendoza-Lorenzob, A. Zentella-Dehesac, E. Méndez-Bolainad, E. Lara-Padillaa, G. Ceballos-Reyesa, P. Cantoe, C. Palma-Florese and R. Coral-Vázqueza. 2012. Caveolae and non-caveolae lipid raft microdomains of human umbilical vein endothelial cells contain utrophin-associated protein complexes. Biochimie, 94:1884-1890.
Ramirez-Sanchez, I., L. Nogueira, A. Moreno, A. Murphy, P. Taub, G. Perkins, G. Ceballos, M. Hogan, M. Malek, and F. Villarreal. 2012. Stimulatory effects of the flavanol (-)-epicatechin on cardiac angiogenesis: Additive effects with exercise. J Cardiovasc Pharmacol, 60:429-438.