Product Sheet CC1015
Description
BACKGROUND c-Met is a tyrosine kinase receptor for Hepatocyte Growth Factor, HGF. This molecule consists of a heterodimer of an extracellular alpha chain disulfide linked to a transmembrane beta chain. The cytoplasmic portion of the beta chain contains the catalytic domain and critical sites for the regulation of its kinase activity.1 c-Met was demonstrated to play an important role in inducing cell migration, invasion, proliferation and survival, in response to its ligand. Upon activation, c-Met initiates several diverse intracellular signaling pathways, including: growth factor receptor-bound protein 2 (Grb2), mitogen-activated protein kinase (MAPK), phosphoinositol 3-kinase (PI3K), and phospholipase C-gamma (PLC-gamma).2 In many human cancers, c-Met is activated via receptor overexpression, amplification, mutation and/or a ligand-dependent autocrine/paracrine loop, as well as the formation of heterodimers with other receptor tyrosine kinases.3 These biochemical and genetic abnormalities correlate with poor clinical outcomes and drug resistance in cancer patients. Targeting c-Met signaling pathway may have significant therapeutic potential.
REFERENCES
1. Christensen, J. G. et al: Cancer Lett., 225:1-26, 2005.
2. Liu, X. et al: Expert Opin Investig Drug 17:997-1011, 2008.
3. Abidoye, O. et al: Rev. Recet Clin. Trails, 2:143-7, 2007.
1. Christensen, J. G. et al: Cancer Lett., 225:1-26, 2005.
2. Liu, X. et al: Expert Opin Investig Drug 17:997-1011, 2008.
3. Abidoye, O. et al: Rev. Recet Clin. Trails, 2:143-7, 2007.
Products are for research use only. They are not intended for human, animal, or diagnostic applications.
A431 cells were stimulated with HGF and subjected to Western Blot analysis using Anti-Phospho-c-Met (Tyr1234/5) Rabbit Polyclonal Antibody (Top), or Anti-c-Met (bottom).
Details
Cat.No.: | CC1015 |
Antigen: | An epitope surrounding and including Tyr1234/5 of human c-Met sequence. |
Isotype: | Rabbit polyclonal IgG |
Species & predicted species cross- reactivity ( ): | Human, Mouse, Rat |
Applications & Suggested starting dilutions:* | WB 1:1000 IP n/d IHC (Paraffin) n/d ICC n/d FACS n/d |
Predicted Molecular Weight of protein: | 140 kDa |
Specificity/Sensitivity: | This antibody detects endogenous phospho-human, mouse and rat c-Met proteins. |
Storage: | Store at -20°C, 4°C for frequent use. Avoid repeated freeze-thaw cycles. |
*Optimal working dilutions must be determined by end user.
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Polyclonal c-Met, Phospho-Tyr1234/1235 Antibody: Polyclonal c-Met, Phospho-Tyr1234/1235 Antibody | Size: 100 ul | CAT.#: CC1015 | Price: $302.00 |