Instructions TF886K
Instructions TF886KS
Description
Transfection, the introduction of nucleic acid into mammalian cells, is a powerful technique used to study in vivo gene function and regulation. The Cytofect™ Neuron Transfection kit is a plasmid DNA delivery system specifically optimized to deliver DNA into Neurons. This kit contains Cytofect-1, Peptide Enhancer and Media for transfection and culturing cells. Peptide Enhancer complexes with DNA and Cytofect-1 and escorts the transfection complex to the nucleus. This results in high transfection efficiency and viability for these traditionally hard-to-transfect primary cells.
#Wells / Rxns
96 / 340
24 / 100
12 / 50
The Kit contains all necessary reagents, media and optimized protocols to carry out successful transfection of Neurons with good efficiency (10-20%) and viability (80%-95%).
Products are for research use only. They are not intended for human, animal, or diagnostic applications. TR1 and PE are manufactured by Targeting Systems.
(Click to Enlarge) Transfection of RCON with a GFP-expression plasmid using CF1 with PE, 1 day (Top) or 3 days (Bottom) post-transfection.
Details
| Kit Contents: | Cytofect-1 Peptide Enhancer (PE) Antibiotics-Free Growth Medium Transfection Medium |
Designed to improve transfection efficiency and cell viability, Cytofect is a plasmid DNA delivery system containing Cytofect-2, Enhancer and Media for transfecting and culturing cells. The Enhancer is an endosomolytic peptide that complexes with DNA and Cytofect-2, then escorts the transfection complex to the nucleus. The results are high transfection efficiency and viability for traditionally hard-to-transfect cells.
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Cytofect Neuron Transfection Kit (100 x 24-Wells): 100 x 24-Well Rxns | Size: 1 Kit | CAT.#: TF886K | Price: $465.00 | |
| Cytofect Neuron Transfection Sample Kit (25 x 24-Wells): 25 x 24-Well Rxns | Size: 1 Sample Kit | CAT.#: TF886KS | Price: $62.00 |
Related Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Cytofect-1 (CF1): Store at -20°C | Size: 250 ul | CAT.#: 051-250 | Price: $175.00 | |
| Cytofect-1 (CF1): Store at -20°C | Size: 500 ul | CAT.#: 051-500 | Price: $283.00 | |
| Cytofect-2 (CF2) : Store at 4°C | Size: 250 ul | CAT.#: 052-250 | Price: $175.00 | |
| Cytofect-2 (CF2) : Store at 4°C | Size: 500 ul | CAT.#: 052-500 | Price: $283.00 | |
| Peptide Enhancer (PE): Store at 4°C | Size: 250 ul | CAT.#: 053-250 | Price: $141.00 | |
| Peptide Enhancer (PE): Store at 4°C | Size: 500 ul | CAT.#: 053-500 | Price: $236.00 | |
| Viral Enhancer (VE): Store at -20°C | Size: 500 ul | CAT.#: 054-500 | Price: $236.00 | |
| Viral Enhancer (VE): Store at -20°C | Size: 250 ul | CAT.#: 054-250 | Price: $141.00 | |
| Cyyofect Transfection Medium: Cyyofect Transfection Medium | Size: 500 ml | CAT.#: TF56-500 | Price: $54.00 |
Resources/Documents
Citations
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2016
Sahoo, S., D. Meijles, I. Ghouleh, M. Tandon, E. Cifuentes-Pagano, J. Sembrat, M. Rojas, E. Goncharova and P. Pagano. 2016. MEF2C-MYOCD and Leiomodin1 Suppression by miRNA-214 Promotes Smooth Muscle Cell Phenotype Switching in Pulmonary Arterial Hypertension. PLoS ONE, dx.doi.org/10.1371/journal.pone.0153780.
2015
Dokun, A., L. Chen, M. Okutsu, C. Farber, S. Hazarika, W. Jones, D. Craig, D. Marchuk, J. Lye, S. Shan, and B. Annex. 2015. ADAM12: A Genetic Modifier of Pre-clinical Peripheral Arterial Disease. Am J Physiol, DOI: 10.1152/ajpheart.00803.2014.
Zelko, I., and R. Folz. 2015. Regulation of Oxidative Stress in Pulmonary Artery Endothelium: Modulation of EC-SOD and NOX4 Expression Using HDAC Class I Inhibitors. Am J Respir Cell Mol Biol, DOI: 10.1165/rcmb.2014-0260OC.
2013
Chen, Z., T.C. Lai, Y.H. Jan, F.M. Lin, W.C. Wang, H. Xiao, Y.T. Wang, W. Sun, X. Cui, Y.S. Li, T. Fang, H. Zhao, C. Padmanabhan, R. Sun, D.L. Wang, H. Jin, G.Y. Chau, H.D. Huang, M. Hsiao, and J.Y.J. Shyy. 2013. Hypoxia-responsive miRNAs target argonaute 1 to promote angiogenesis. The Journal of Clinical Investigation. 123:1057-1067.
He, M., X. Liang, L. He, W. Wen, S. Zhao, L. Wen, Y. Liu, J.Y.J. Shyy, and Z. Yuan. 2013. Endothelial dysfunction in rheumatoid arthritis: the role of monocyte chemotactic protein-1-induced protein. Arteriosclerosis, Thrombosis, and Vascular Biology. 33:1384-1391.
Yan, Y., X. Li, K. Kover, M. Clements, and P. Ye. 2013. CREB participates in the IGF‐I‐stimulation cyclin D1 transcription. Developmental Neurobiology. 73:559-570.
2012
Jeon, Y.J., M.G. Jo, H.M. Yoo, S.H. Hong, J.M. Park, S.H. Ka, K.H. Oh, J.H. Seol, Y.K. Jung, and C.H. Chung. 2012. Chemosensitivity is controlled by p63 modification with ubiquitin-like protein ISG15. The Journal of Clinical Investigation. 122:2622-2636.