MSDS Cryopreserved Cells
Instructions HLMVEC Normal
5 Important Cell Culture Rules
Cell Apps Flyer Airway Cells
Cell Apps Flyer Cardiovascular Cells
Cell Apps Flyer Endothelial Cells
Cell Apps Poster Primary Cells
Description
Human Lung Microvascular Endothelial Cells (HLMVEC) provide a useful tool for studying various aspects of pathology and biology of the pulmonary microvasculature in vitro. The pulmonary vasculature is of great physiological/pathological significance. It is now recognized to be a tissue composed of metabolically active, functionally responsive cells, that interact with circulating substrates and regulate the composition of systemic arterial blood, affect target organ functions, and contribute to thrombosis, hemostasis and immune reactions, as well as tumor metastasis.
HLMVEC from Cell Applications, Inc. have been used to elucidate the therapeutic effects of Angiotensin I-converting enzyme (ACE) inhibitors, and the results revealed that they provide an additional benefit to patients by activating bradykinin B1 receptor leading to prolonged nitric oxide (NO) production in endothelial cells and inhibition of PKCϵ.
Pre-Screened Human Lung Microvascular Endothelial Cells (S-HLMVEC) are select lots of HLMVEC that have been undergone further characterization. Pre-Screened HLMVEC have been stimulated by VEGF, and assayed for activation of VEGFR2 signaling pathway. More information about pre-screened endothelial cells can be found on the Pre-Screened Endothelial Cell Product Page.
(Click to Enlarge) Human Lung Microvascular Endothelial Cells (HLMVEC) in culture (A). HLMVEC immunolabeled for CD31/PECAM (green); nuclei are visualized with PI (red) (B). HLMVEC transfected with GFP plasmid DNA using the Cytofect™ Endothelial Cell Transfection Kit (C,D)
Details
Tissue | Human lung (Nondiseased, Asthma (AS), or Type 2 Diabetes (T2D)) |
QC | No bacteria, yeast, fungi, mycoplasma, virus |
Character | Factor VIII-related Ab, CD31 (PECAM-1), DiI-Ac-LDL uptake. S-HLMVEC are select HLMVEC lots that have been tested positive for VEGFR2 pathway activation following stimulation by VEGF. |
Bioassay | Attach, spread on AFS-coated surface, proliferate in Growth Med |
Cryovial | 500,000 HLMVEC (3rd passage) frozen in Basal Medium w/ 10% FBS, 10% DMSO |
Kit | Cryovial HLMVEC (540-05, 540AS-05, or 540T2D-05), Gr Med (111-500), Attchmnt Fctr Soln (123-100), Sbcltr Rgnt Kit (090K) |
Proliferating | Shipped in Tsfr Med, 4th psg (flasks or plates) |
Doublings | At least 15 |
Applications | Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use. |
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Cryopreserved Human Lung Microvascular Endothelial Cells Total Kit, adult: 5x10^5 Cells (Adult), Medium & Subculture Reagents (See Details tab for specifics) | Size: 1 Kit | CAT.#: 540K-05a | Price: $1,023.00 | |
| Cryopreserved Lung Microvascular Endothelial Cells (HLMVEC), adult: Frozen HLMVEC (5x10^5) | Size: 1 Cryovial | CAT.#: 540-05a | Price: $765.00 | |
| Cryopreserved Human Lung Microvascular Endothelial Cells with Asthma Total Kit, adult: 5x10^5 Cells (from donor with Asthma, Adult), Medium & Subculture Reagents (See Details tab for specifics) | Size: 1 Kit | CAT.#: 540ASK-05a | Price: $1,183.00 | |
| Cryopreserved Lung Microvascular Endothelial Cells, Asthma (HLMVEC-AS), adult: Frozen HLMVEC-AS from donor with Asthma (5x10^5) | Size: 1 Cryovial | CAT.#: 540AS-05a | Price: $925.00 | |
| Cryopreserved Human Lung Microvascular Endothelial with Diabetes Cells Total Kit, adult: 5x10^5 Cells (from donor with Type 2 Diabetes, Adult), Medium & Subculture Reagents (See Details tab for specifics) | Size: 1 Kit | CAT.#: 540T2DK-05a | Price: $1,134.00 | |
| Cryopreserved Lung Microvascular Endothelial Cells, Type 2 Diabetes (HLMVEC-T2D), adult: Frozen HLMVEC-T2D from donor with Type 2 Diabetes (5x10^5) | Size: 1 Cryovial | CAT.#: 540T2D-05a | Price: $876.00 | |
| Cryopreserved Pre-Screened Human Lung Microvascular Endothelial Cells Total Kit: 5x10^5 Cells (Pre-Screened, Adult), Medium & Subculture Reagents (See Details tab for specifics) | Size: 1 Kit | CAT.#: S540K-05a | Price: $1,184.00 | |
| Cryopreserved Lung Microvascular Endothelial Cells (HLMVEC), Pre-Screened, adult: Frozen HLMVEC (5x10^5) | Size: 1 Cryovial | CAT.#: S540-05a | Price: $926.00 | |
| Proliferating Lung Microvascular Endothelial Cells (HLMVEC), adult: Actively growing, dividing cells in medium | Size: T-25 Flask | CAT.#: 541-25a | Price: $765.00 | |
| Proliferating Lung Microvascular Endothelial Cells (HLMVEC), adult: Actively growing, dividing cells in medium | Size: T-75 Flask | CAT.#: 541-75a | Price: $955.00 | |
| Proliferating Lung Microvascular Endothelial Cells (HLMVEC), adult: Actively growing, dividing cells in medium | Size: 96 Well | CAT.#: 541-96Wa | Price: $1,075.00 | |
| Proliferating Lung Microvascular Endothelial Cells (HLMVEC), adult: Actively growing, dividing cells in medium | Size: 24 Well | CAT.#: 541-24Wa | Price: $955.00 |
Related Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Microvascular Endothelial Cell Growth Medium: All-in-one ready-to-use | Size: 500 ml | CAT.#: 111-500 | Price: $148.00 | |
| Microvascular Endothelial Cell Growth Medium Kit: Basal medium & growth supplement sold together packaged separately | Size: Yields 500 ml | CAT.#: 111K-500 | Price: $158.00 | |
| Microvascular Endothelial Cell Basal Medium: Basal medium (contains no growth supplement). Add GS before use. | Size: 500 ml | CAT.#: 100-500 | Price: $74.00 | |
| Microvascular Endothelial Cell Growth Supplement: Added to Basal Medium to create Growth Medium | Size: 30 ml | CAT.#: 111-GS | Price: $105.00 | |
| Microvascular Endothelial Cell Growth Medium Kit, components separate: Basal medium & growth supplement sold, together packaged separately. Supplements in individual vials. | Size: Yields 500 ml | CAT.#: 111KS-500 | Price: $160.00 |
Extended Family Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Anti-ICAM-1: Rabbit Intercellular Adhesion Molecule-1 Antibody | Size: 100 ul | CAT.#: CG1238 | Price: $275.00 | |
| Anti-VEGF: Rabbit Vascular Endothelial Growth Factor Antibody | Size: 100 ul | CAT.#: CG1634 | Price: $275.00 | |
| Polyclonal Vascular Endothelial Growth Factor-C Antibody: Polyclonal Vascular Endothelial Growth Factor-C Antibody | Size: 100 ul | CAT.#: CB3778 | Price: $302.00 | |
| Polyclonal VEGF Receptor 1 Antibody: Polyclonal VEGF Receptor 1 Antibody | Size: 100 ul | CAT.#: CB3839 | Price: $333.00 | |
| Attachment Factor Solution (AFS): AFS, contains Bovine Gelatin | Size: 100 ml | CAT.#: 123-100 | Price: $22.00 | |
| Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO. | Size: 50 ml | CAT.#: 040-50 | Price: $54.00 | |
| Cyto-X Cell Counting Reagent: 500 tests | Size: 1 Bottle | CAT.#: 028-01 | Price: $160.00 | |
| Cyto-X Cell Counting Reagent Sample: 100 tests | Size: Sample | CAT.#: 028-S | Price: $41.00 | |
| Cytofect Endothelial Cell Transfection Kit (250 x 24-Wells): 250 x 24-Well Rxns | Size: 1 Kit | CAT.#: TF101K | Price: $496.00 | |
| Cytofect Endothelial Cell Transfection Sample Kit (25 x 24-Wells): 25 x 24-Well Rxns | Size: 1 Sample Kit | CAT.#: TF101KS | Price: $62.00 | |
| Human E-Selectin ELISA Kit: Human E-Selectin ELISA Kit | Size: 96 Wells | CAT.#: CL0501 | Price: $587.00 | |
| Lung Microvascular Endothelial Cell RNA (HLMVEC RNA), Adult: Total RNA prepared from Human Lung Microvascular Endothelial Cells, adult | Size: 10 ug | CAT.#: 540-R10a | Price: $465.00 | |
| Lung Microvascular Endothelial Cell RNA (HLMVEC RNA), Adult: Total RNA prepared from Human Lung Microvascular Endothelial Cells, adult | Size: 25 ug | CAT.#: 540-R25a | Price: $930.00 | |
| Human ICAM-1 ELISA Kit: Human Intercellular Adhesion Molecule-1 ELISA Kit | Size: 96 Wells | CAT.#: CL0370 | Price: $484.00 | |
| Human Gamma-Interferon Inducible Protein 10 (IP-10 / CXCL10): Human gamma-Interferon Inducible Protein 10 | Size: 25 ug | CAT.#: RP1127-25 | Price: $194.00 | |
| Human Gamma-Interferon Inducible Protein 10 (IP-10 / CXCL10): Human gamma-Interferon Inducible Protein 10 | Size: 100 ug | CAT.#: RP1127-100 | Price: $484.00 | |
| Human Lung RNA: Total RNA prepared from human lung tissue | Size: 50 ug | CAT.#: 1H40-50 | Price: $158.00 | |
| Human Gamma-Interferon Inducible Protein 10 (IP-10 / CXCL10): Human gamma-Interferon Inducible Protein 10 | Size: 1000 ug | CAT.#: RP1127-1000 | Price: $3,175.00 | |
| Human Lung RNA: Total RNA prepared from human lung tissue | Size: 250 ug | CAT.#: 1H40-250 | Price: $595.00 | |
| Human P-Selectin ELISA Kit: Human P-Selectin ELISA Kit | Size: 96 wells | CAT.#: CL0505 | Price: $517.00 | |
| Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution | Size: 1 Kit | CAT.#: 090K | Price: $63.00 | |
| Human Vascular Endothelial Growth Factor-121 (VEGF-121): Human Vascular Endothelial Growth Factor-121 | Size: 10 ug | CAT.#: RP1116-10 | Price: $194.00 | |
| Human Vascular Endothelial Growth Factor-121 (VEGF-121): Human Vascular Endothelial Growth Factor-121 | Size: 100 ug | CAT.#: RP1116-100 | Price: $484.00 | |
| Human Vascular Endothelial Growth Factor-121 (VEGF-121): Human Vascular Endothelial Growth Factor-121 | Size: 1000 ug | CAT.#: RP1116-1000 | Price: $4,090.00 | |
| Human VEGF-c ELISA Kit: Human Vascular Endothelial Growth Factor C ELISA Kit | Size: 96 Wells | CAT.#: CL0588 | Price: $581.00 | |
| Human VEGF-121, Animal-Free: Human Vascular Endothelial Growth Factor-121, Animal-Free | Size: 10 ug | CAT.#: RP1116AF-10 | Price: $213.00 | |
| Human VEGF-121, Animal-Free: Human Vascular Endothelial Growth Factor-121, Animal-Free | Size: 100 ug | CAT.#: RP1116AF-100 | Price: $533.00 | |
| Human VEGF-121, Animal-Free: Human Vascular Endothelial Growth Factor-121, Animal-Free | Size: 1000 ug | CAT.#: RP1116AF-1000 | Price: $4,499.00 |
Resources/Documents
Citations
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2015
Bai, L., H. Andersson, K. McConnell, M. Hernandez, J. Gonzalez, X. Liu, S. La Francesca, J. Sakamoto and R. Serda. 2015. Silencing of Tumor Necrosis Factor Receptor-1 in Human Lung Microvascular Endothelial Cells Using Particle Platforms for siRNA Delivery. Current Drug Targets, 16:1531-1539.
Li, C., C. Gonsalves, M. Eiymo Mwa Mpollo, P. Malik, S. Tahara, and V. Kalra. 2015. MicroRNA 648 Targets ET-1 mRNA and Is Cotranscriptionally Regulated with MICAL3 by PAX5. Molecular and Cellular Biology, 35:514-528.
2014
Li, C., M. Mpollo, C. Gonsalves, S. Tahara, P. Malik and V. Kalra. 2014. Peroxisome Proliferator-activated Receptor-α-mediated Transcription of miR-199a2 Attenuates Endothelin-1 Expression via Hypoxia-inducible Factor-1α. J Biol Chem, 289:36031-36047.
2006
Stanisavljevic, S., T. Ignjatovic, P.A. Deddish, V. Brovkovych, K. Zhang, E.G. Erdös, and R.A. Skidgel. 2006. Angiotensin I-Converting Enzyme Inhibitors Block Protein Kinase Cϵ by Activating Bradykinin B1 Receptors in Human Endothelial Cells. Journal of Pharmacology and Experimental Therapeutics. 316:1153-1158
2004
Ignjatovic, T., S. Stanisavljevic, V. Brovkovych, R.A. Skidgel, and E.G. Erdos. 2004. Kinin B1 Receptors Stimulate Nitric Oxide Production in Endothelial Cells: Signaling Pathways Activated by Angiotensin I-Converting Enzyme Inhibitors and Peptide Ligands. Molecular Pharmacology. 66:1310-1316.