MSDS Cryopreserved Cells
5 Important Cell Culture Rules
Cell Apps Poster Primary Cells
Cell Applications Inc Brochure
Instructions MHSC
Description
Mouse Hepatic Stellate Cells (MHSC) are one of the important cell type in the liver. Hepatic Stellate Cells play an important role in liver homeostasis, maintenance and regeneration including retinol metabolism. Activation of stellate cells in disease state leads to liver fibrosis, hepatitis B & C, fatty liver disease, diabetes and other chronic diseases.
Hepatic Stellate Cells are primary cells derived from mouse liver and cultured in optimized Hepatic Stellate Cells Growth Medium. MHSCs from Cell Applications, Inc. provide an excellent model system to study many aspects of liver function, metabolism and pathophysiology. Primary culture of Hepatic Stellate Cells is suitable for many cell-based assays including toxicity, drug screening and metabolism and is a valuable tool to study liver function, physiology and liver diseases.
Characterization: Positive for α-smooth muscle actin by ICC staining.
Mouse Hepatic Stellate Cells: MHSC Left: phase contrast, Right: HHF immunolabeled for α-smooth muscle actin (green), nuclei are stained with DAPI.
Details
| Tissue | Normal healthy mouse liver tissue |
| QC | No bacteria, yeast, fungi, mycoplasma, virus |
| Bioassay | Attach, spread, proliferate in Growth Med |
| Cryovial | 500,000 MHSC (3rd passage) frozen in Basal Medium w/ 10% FBS, 10% DMSO |
| Kit | Cryovial frozen MHSC (M782-05a), Growth Medium (M7117-500), Subcltr Rgnt Kit (090K) |
| Proliferating | Shipped in Gr Med, 3rd psg (flasks or plates) |
| Doublings | At least 10 |
| Applications | Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use. |
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Cryopreserved Mouse Hepatic Stellate Cells Total Kit: Cryopreserved MHSC Total Kit: 5x10^5 Cells (Adult), Medium & Subculture Reagents (See Details tab for specifics) | Size: 1 Kit | CAT.#: M782K-05a | Price: $673.00 | |
| Cryopreserved Mouse Hepatic Stellate Cells (MHSC), adult: Frozen MHSC (5x10^5) | Size: 1 Cryovial | CAT.#: M782-05a | Price: $495.00 | |
| Proliferating Mouse Hepatic Stellate Cells (MHSC), adult: Actively growing, dividing cells in medium | Size: T-25 Flask | CAT.#: M783-25a | Price: $495.00 | |
| Proliferating Mouse Hepatic Stellate Cells (MHSC), adult: Actively growing, dividing cells in medium | Size: T-75 Flask | CAT.#: M783-75a | Price: $685.00 | |
| Proliferating Mouse Hepatic Stellate Cells (MHSC), adult: Actively growing, dividing cells in medium | Size: 96 Well | CAT.#: M783-96Wa | Price: $805.00 |
Related Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Mouse Hepatic Stellate Cells Growth Medium: All-in-one ready-to-use | Size: 500 ml | CAT.#: M7117-500 | Price: $125.00 | |
| Mouse Hepatic Stellate Cells Growth Medium Kit: Basal medium & growth supplement sold together packaged separately | Size: Yields 500 ml | CAT.#: M7117K-500 | Price: $135.00 | |
| Mouse Hepatic Stellate Cells Basal Medium: Basal medium (contains no growth supplement). Add GS before use. | Size: 500 ml | CAT.#: M7116-500 | Price: $74.00 | |
| Mouse Hepatic Stellate Cells Growth Supplement: Added to Basal Medium to create Growth Medium | Size: 50 ml | CAT.#: M7117-GS | Price: $68.00 |
Extended Family Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution | Size: 1 Kit | CAT.#: 090K | Price: $63.00 | |
| Cyto-X Cell Counting Reagent: 500 tests | Size: 1 Bottle | CAT.#: 028-01 | Price: $160.00 | |
| Cyto-X Cell Counting Reagent Sample: 100 tests | Size: Sample | CAT.#: 028-S | Price: $41.00 |