Description
Alveolar type 2 cells (AEC2) function in ion transport and epithelium repair, as well as in the defense and immune response against microorganisms. AEC2 secrete surfactant proteins, release a host of factors that include cytokines and chemokines, and present antigen to specific T cells.
Cell Applications' AEC2 / i-HAEpC2 are Type 2 Alveolar Epithelial Cells derived from induced pluripotent stem cells with our unique DISCUSTM 3D system. iPSC undergo multistage differentiation to AEC2, and are characterized by staining for TTF-1,1 a marker specific for bronchial and alveolar cells, and Surfactant protein C (SFTPC), a marker for Alveolar Type 2 cells.
Endodermal tissues such as intestine, liver and lung reach full function only in a 3-D environment. Cell Applications' i-HAEpC2 are typically >90% positive for ACE2 receptor. The marker is only present when cells are in the DISCUSTM 3D system and is rapidly lost if subjected to 2D plating.
The i-Alveolar Epithelial Cell Total Kit includes cryopreserved AEC2 / i-HAEpC2, optimized growth medium, DISCUSTM 3D gel kit, dissociation solution, and other supporting reagents.
*DISCUS™ is a cell-specific system of 3D discs that address the challenges of anoxia and diffusion limits in 3D tissue engineering. Measuring 3mm across and only 600 microns in depth at the thickest point, these versatile units need only common, inexpensive laboratory equipment to generate your 3D engineered tissue of choice.
Reference:
1. D Fabbro et al. Eur J Cancer. 1996 Mar;32A(3):512-7.
Top Row: 3D culture of AEC2 / i-HAEpC2 organoids embedded in the DISCUSTM system.
Midde Row: Immunostaining of AEC2 / i-HAEpC2 organoids for ACE2/DAPI (green/blue), SFTPC/DAPI (red/blue), and ACE2/SFTPC (green/red).
Bottom Row: Immunostaining of AEC2 / i-HAEpC2 organoids for ACE2/DAPI (green/blue), TTF-1/DAPI (red/blue), and ACE2/TTF-1 (green/red).
Details
| Source | Derived from Human iPSC of a single donor |
| QC | No bacteria, yeast, fungi, mycoplasma, virus |
| Bioassay | Positive for ACE2 staining. |
| Cryovial | 500,000 AEC2 / i-HAEpC2 (1st passage) in freezing medium |
| Kit | Cryovial frozen AEC2 / i-HAEpC2 (i538-05), Gr Med Kit (i51103K-100), i-HAEpC2 DISCUSTM Gel Kit (DISC1054K), Wash Buffer (077-25), DISCUSTM Dissociation Solution (DISC1050), Non-Stick Solution (1025-05). |
| Cultured | Contact Cell Applications tech support for custom cultures |
| Doublings | The cells cannot be passaged |
| Applications | Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use. |
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Cryopreserved Human iPSC-derived Alveolar Epithelial Cells (AEC2/i-HAEpC2) Total Kit: 5x10^5 Cells, Growth Media Kit, i-HAEpC2 DISCUS Gel Kit, Wash Buffer, DISCUS Dissociation Solution, Non-Stock Solution (See Details tab for specifics). | Size: 1 Kit | CAT.#: i538K-05 | Price: $1,693.00 | |
| Cryopreserved iPSC-derived Alveolar Epithelial Cells (AEC2/i-HAEpC2): Frozen i-HAEpC2 (5x10^5) | Size: 1 Cryovial | CAT.#: i538-05 | Price: $1,475.00 |
Related Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| AEC2/iHAEpC2 Growth Medium Kit: Basal medium & growth supplement sold together packaged separately. Keep GS frozen for up to 6 months. Once thawed, use within 4 weeks. | Size: Yields 100ml | CAT.#: i51103K-100 | Price: $182.00 | |
| AEC2/iHAEpC2 Basal Medium: Without growth supplement (GS). Add GS before use. | Size: 100 ml | CAT.#: i51102-100 | Price: $46.00 | |
| AEC2/iHAEpC2 Growth Supplement: Added to Basal Medium to create Growth Medium. | Size: 3 ml | CAT.#: i51103-GS | Price: $160.00 | |
| i-HAEpC2 DISCUS 3D Gel Kit: Cell Applications 3D system for iPSC-derived human alveolar epithelial cells, type 2 | Size: 1 Kit | CAT.#: DISC1054K | Price: $68.00 | |
| DISCUS Dissolution Reagent: Reagent for DISCUS dissolution. | Size: 10 ml | CAT.#: DISC1050 | Price: $32.00 | |
| DISCUS Non-Stick Solution: Reagent for cell culture. | Size: 5 ml | CAT.#: 1025-05 | Price: $32.00 |
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