Description
For the first time, Cell Applications, Inc. makes Human Brown Fat Preadipocytes (i-HBrPAd) available for research. Until now, studying human brown fat was nearly impossible due to its extreme scarcity in adults. But with our exclusive i-HBrPAd, derived from pluripotent stem cells and cryopreserved before maturation, researchers finally have direct access to this critical brown adipose tissue.
Using our Preadipocyte Differentiation and Maturation Medium, i-HBrPAd cells fully mature in just 14 days and remain healthy and responsive for up to two weeks post-differentiation. These fat cells are over 97% positive for PRDM16, the essential thermogenic factor in brown fat development, and show the multilocular adipose vesicles unique to brown fat upon Oil Red O staining.
With Cell Applications’ Brown Fat, researchers can now explore new frontiers in obesity, weight management and diabetes treatments and gain insights into brown fat development and its potential for in vivo generation. This world-first innovation changes what’s possible in metabolic research.
i-HBrPAd after two weeks of maturation.
Upper left: Oil Red O stain.
Upper right: unstained cells with lipid droplets.
Lower left: PRDM16 (green), nuclei (DAPI).
Lower right: flow cytometry of mature HBrAd stained with PRDM16.
Details
| Source | Derived from Human iPSC of a single donor. |
| QC | No bacteria, yeast, fungi, mycoplasma, virus. |
| Bioassay | Positive for PRDM16 staining. |
| Cryovial | Set of three cryovials (each yielding approximately 100,000 attached, viable cells), for ~300,000 total equivalent of i-HBrPAd (first passage), in freezing medium. Three million total cells are included in Cat# i804-30 and i804K-30, and approximately 10% (300,000) viable cells attach. |
| Kit | Cryovial frozen i-HBrPAd cells (i804-30), i-HBrPAd Thawing Medium (i8117T-30), i-HBrPAd Differentiation Medium Kit (i8117DK-15), i-HBrPAd Expansion Medium Kit (i8117EK-10), i-HBrPAd Maturation Medium Kit (i8117MK-50), HiPSC Xeno-Free Coating Solution Stock (126XF-005). |
| Cultured | Contact us for availability. |
| Doublings | The cells are non-proliferative. |
| Applications | Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use. |
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Cryopreserved Human Brown Fat Preadipocytes ( i-HBrPAd) Total Kit, iPSC-derived: The Total Cell Kit includes cryopreserved cells, and the medium and reagents required to establish the cell culture. (See details tab for a summary of included items) | Size: 1 Kit | CAT.#: i804K-30 | Price: $1,138.00 |