Product Sheet CC10021
Description
BACKGROUND The Jumonji (Jmj) gene was identified by a mouse gene trap approach and has essential roles in the development of multiple tissues. The Jmj protein has a DNA binding domain, ARID, and two conserved Jmj domains (JmjN and JmjC).
Several proteins in the Jumonji family are involved in transcriptional repression and/or chromatin regulation.1 Recently, human members of the family have been shown to be a histone demethylases, and the JmjC domain is essential for demethylase activity.2
Jumonji domain containing 1B (JMJD1B, also known as KDM3B/JHDM2B) contains a highly-conserved C-terminus, which includes a zinc finger with the unique spacing Cys-X2-Cys-X7-His-X2-Cys-X2-Cys-X4-Cys-X2-Cys and a JmjC domain. JMJD1B has been shown to be a histone demethylase responsible for demethylating mono methyl histone H3 at K9.3 Methylation of H3-K4 and H3-K9 is linked to transcriptional activation and repression, respectively. JMJD1B plays an important role in chromatin regulation and gene expression, and controls development through various signaling pathways. It may also be a candidate for tumor suppressors.
REFERENCES
REFERENCES
1. Jung J et al.: Dev Dyn. 232:21-32, 2005.
2. Shin S & Janknecht R: Biochem Biophys Res Commun. 353:973-7, 2007.
3. Natoli G et al.: Curr. Opin. Drug Dis. Dev. 12:607-615, 2009.
2. Shin S & Janknecht R: Biochem Biophys Res Commun. 353:973-7, 2007.
3. Natoli G et al.: Curr. Opin. Drug Dis. Dev. 12:607-615, 2009.
Products are for research use only. They are not intended for human, animal, or diagnostic applications.
(Click to Enlarge) Western Blot detection of endogenous JMJD1B protein in normal primary cell lysates using JMJD1B Antibody. HCF: Human Cardiac Fibroblasts. ROb: Rat Osteoblasts.
Details
Cat.No.: | CC10021 |
Antigen: | Human JMJD1B C-terminal protein sequence. |
Isotype: | Mouse IgG2a |
Species & predicted species cross- reactivity ( ): | Human, Rat, Mouse |
Applications & Suggested starting dilutions: | WB 1:1000 IP 1:50 IHC 1:100 ICC n/d FACS n/d |
Predicted Molecular Weight of protein: | 195 kDa |
Specificity/Sensitivity: | Detects endogenous levels of JMJD1B protein in various cell lysates. |
Storage: | Store at 4° C for frequent use; at -20° C for at least one year. |
*Optimal working dilutions must be determined by end user.
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Monoclonal JMJD1B Antibody: Monoclonal JMJD1B Antibody | Size: 100 ul | CAT.#: CC10021 | Price: $302.00 |