Product Sheet CG1396
Description
BACKGROUND The p70 S6 kinase was originally identified as a kinase that regulates serine phosphorylation of the 40 S ribosomal S6 protein, a major component of the machinery involved in protein synthesis in mammalian cells. This kinase is activated by numerous mitogens, growth factors, and hormones. Activation of p70S6K occurs through the phosphorylation at a number of sites.
p70S6K activation has been implicated in cell cycle control, cell movement, and cell differentiation regulation.1 It has been well demonstrated that the activation of p70 S6 kinase is regulated by the FKBP 12-rapamycin-associated protein (FRAP/ mTOR), whose activation is in turn regulated by the upstream activation of the phosphatidylinositol 3′-kinase pathway, typically activated upon stimulation of a cell with a growth factor (such as IGF-1).2 Branched chain amino acids, such as leucine, are also sufficient for the activation of mTOR, resulting in an increase in p70S6K phosphorylation.3 On the other hand, AMPK plays important role in negatively regulating mTOR/p70 S6 kinase pathway.4
REFERENCES
1. Pearson RB & Thomas G: Prog. Cell Cycle Res. 1:21-32, 1995.
2. Chung J et al.: Nature 370:71-75, 1994.
3. Ban H et al.: Int J Mol Med. 13:537-43, 2004.
4. Chan AYM & Dyck JRB: Can. J. Physiol. Pharmacol. 83:24–28, 2005.
2. Chung J et al.: Nature 370:71-75, 1994.
3. Ban H et al.: Int J Mol Med. 13:537-43, 2004.
4. Chan AYM & Dyck JRB: Can. J. Physiol. Pharmacol. 83:24–28, 2005.
Products are for research use only. They are not intended for human, animal, or diagnostic applications.
(Click to Enlarge)
Top: Immunoblotting analysis of extracts from Jurkat cells, using Anti-p70 S6 Kinase antibody. The lane on the left was treated with the Anti-p70 S6 Kinase antibody. The lane on the right (negative control) was treated with both Anti-p70 S6 Kinase antibody and the synthesized immunogen peptide. Middle: Immunohistochemistry analysis of paraffin-embedded human brain tissue using Anti-p70 S6 Kinase antibody. Cells on the left were treated with the Anti-p70 S6 Kinase antibody. Cells on the right (negative control) were treated with both Anti-p70 S6 Kinase antibody and the synthesized immunogen peptide. Bottom: Immunofluorescence of HeLa cells using Anti-p70 S6 Kinase antibody. Cells on the left were treated with the Anti-p70 S6 Kinase antibody. Cells on the right (negative control) were treated with both Anti-p70 S6 Kinase antibody and the synthesized immunogen peptide.
Details
Cat.No.: | CG1396 |
Antigen: | Synthesized peptide derived from human p70 S6 Kinase. |
Isotype: | Rabbit IgG |
Species & predicted species cross- reactivity ( ): | Human, Mouse, Rat |
Applications & Suggested starting dilutions:* | WB 1:500-1:1000 IP n/d IHC 1:50-1:100 ICC n/d FACS n/d IF 1:500-1:1000 |
Predicted Molecular Weight of protein: | 59 kDa |
Specificity/Sensitivity: | Detects endogenous p70 S6 Kinase proteins without cross-reactivity with other family members. |
Storage: | Store at -20°C, 4°C for frequent use. Avoid repeated freeze-thaw cycles. |
*Optimal working dilutions must be determined by end user.
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Rabbit p70 S6 Kinase Antibody: Rabbit p70 S6 Kinase Antibody | Size: 100 ul | CAT.#: CG1396 | Price: $384.00 |