Product Sheet CG1153
Description
BACKGROUND The estrogen-related receptor alpha (ERRalpha, NR3B1) is an orphan member of the nuclear receptor superfamily identified on the basis of its structural similarity to the estrogen receptor alpha (ERalpha, NR3A1). There are three members of the ERR family, ERRalpha, ERRbeta, and ERRgamma. ERRalpha is functionally similar to ERalpha in that it can bind the inverted repeat estrogen response element (ERE) sequence in target gene promoters; although ERRalpha preferentially binds a nine-nucleotide extended half site sequence, the ERRE. In contrast to the ligand-dependent activation of ERalpha, ERRalpha is constitutively active and does not respond to estradiol or other natural estrogens, although its activity can be inhibited by the synthetic estrogen diethylstilbestrol.1 The constitutive ERRalpha can interact with and be modulated by members of the SRC and PGC-1 families of coactivators.2 The function of ERRalpha as a transcriptional activator seems to be cell type and promoter specific. This specificity is proposed to be dependent on factors including relative coactivator levels, interactions with other nuclear receptors, and the presence of appropriate activating stimuli or the context of the response elements. ERRalpha activates the promoter of its own gene, ESRRA, thereby providing positive regulation of its own expression.3 Other known ERRalpha target genes are involved in regulation of mitochondrial biogenesis and energy metabolism. ERRalpha also activates the TFF1 (also known as pS2) and aromatase genes, which are implicated in breast cancer. ERRalpha is also a phospho-protein whose expression in human breast tumors correlates with that of the receptor tyrosine kinase ErbB2, suggesting that its transcriptional activity could be regulated by signaling cascades.4 Growth factor signaling can selectively activate ERRalpha target genes in breast cancer cells.
REFERENCES
1. Giguere, V. et al: Nature 331:91-4, 1988
3. Sladek, R. et al: Mol. Cell. Biol. 17:5400-9, 1997
2. Huss, J.M. et al: Mol. Cell. Biol. 24:9079-91, 2004
4. Barry, JB. & Giguere, V.: Cancer Res. 65:6120-6129, 2006
3. Sladek, R. et al: Mol. Cell. Biol. 17:5400-9, 1997
2. Huss, J.M. et al: Mol. Cell. Biol. 24:9079-91, 2004
4. Barry, JB. & Giguere, V.: Cancer Res. 65:6120-6129, 2006
Products are for research use only. They are not intended for human, animal, or diagnostic applications.
Details
Cat.No.: | CG1153 |
Antigen: | Synthetic peptide M(1)SSQVVGIEPLYIKAE(16) corresponding to amino acid residues 1-16 from human ERR1 protein. |
Isotype: | Rabbit IgG |
Species & predicted species cross- reactivity ( ): | Human |
Applications & Suggested starting dilutions:* | WB 2 ug/mL IP n/d IHC n/d ICC n/d FACS n/d |
Predicted Molecular Weight of protein: | 52 kDa |
Specificity/Sensitivity: | Detects endogenous ERR-α proteins without cross-reactivity with other family members. |
Storage: | Store at -20°C, 4°C for frequent use. Avoid repeated freeze-thaw cycles. |
*Optimal working dilutions must be determined by end user.
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Rabbit ERR-Alpha Antibody: Rabbit ERR-alpha Antibody | Size: 100 ul | CAT.#: CG1153 | Price: $461.00 |