Product Sheet CP10092
Description
BACKGROUND The ETS family of transcription factors, characterized by an evolutionarily conserved DNA-binding domain, regulates expression of more than 300 target genes by binding to a purine-rich GGAA/T core sequence. Depending on the cellular context, they can function as transactivators or transrepressors. Ets proteins have been implicated in regulation of gene expression during a variety of biological processes, including growth control, transformation, T-cell activation, and developmental programs in many organisms. Signals regulating cell growth are transmitted from outside the cell to the nucleus by growth factors and their receptors, G-proteins, kinases and transcription factors. It was shown that ETS signal transduction is implicated in hematopoiesis and angiogenesis at the earliest stages of embryogenesis, and is later involved in tissue development. Deregulated expression and/or formation of chimeric fusion proteins of the ETS family due to proviral insertion or chromosome translocation is associated with leukemias and with specific types of solid tumors.1
Among the multiple Ets proteins, the PEA3 group consists of ETV1 (Ets variant gene 1; also called ER81), ETV4 (also called PEA3) and ETV5 (also called ERM). All three members are 95% identical in the ETS domain and more than 85% in the acidic transactivation domain. Several studies suggest that the PEA3 group proteins are involved in intestinal tumors, gastric cancer, and breast cancer metastasis. In nearly all Ewing’s sarcoma tumors, EWS, which encodes a RNA-binding protein, is fused by chromosomal translocation to an Ets gene, including FLI, ERG, ETV4, and ETV1. This results in the expression of chimeric proteins that may be important in tumor cell transformation.2 Recently, it was reported that TMPRSS2, an AR-regulated gene, is fused by translocation to the ETV1, ERG, or ETV4 gene in a subset of prostate cancers. These findings suggest an important role for PEA3 proteins in prostate cancer.3
REFERENCES
1. Wasylyk, B. et al: Eur. J. Biochem. 211:7-18, 2005
2. Maher, C.A. et al: Nature 458:97-101,2009
3. Cai, C. et al: Mol. Endocrinol.25:1835-46, 2007
2. Maher, C.A. et al: Nature 458:97-101,2009
3. Cai, C. et al: Mol. Endocrinol.25:1835-46, 2007
Products are for research use only. They are not intended for human, animal, or diagnostic applications.
(Click to Enlarge) Western Blot detection of ETV1 proteins in 293 cell lysate containing overexpressed hIgGFc-hETV1 fragment fusion proteins (90 kDa) using ETV1 Antibody.
Details
Cat.No.: | CP10092 |
Antigen: | Purified recombinant human ETV1 fragment expressed in 293 cells. |
Isotype: | Mouse IgG |
Species & predicted species cross- reactivity ( ): | Human, Mouse, Rat |
Applications & Suggested starting dilutions:* | WB 1:1000 IP n/d IHC n/d ICC n/d FACS n/d |
Predicted Molecular Weight of protein: | 55 kDa |
Specificity/Sensitivity: | Detects overexpressed ETV1 proteins without cross-reactivity with other family members. |
Storage: | Store at -20°C, 4°C for frequent use. Avoid repeated freeze-thaw cycles. |
*Optimal working dilutions must be determined by end user.
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Mouse ETV1 Antibody: Mouse ETV1 Antibody | Size: 100 ul | CAT.#: CP10092 | Price: $333.00 |