Product Sheet CG1409
Description
BACKGROUND Poly (ADP-ribose) polymerase (PARP) catalyzes the post-translational modification of proteins by the addition of multiple ADP-ribose moieties. PARP transfers ADP-ribose from nicotinamide dinucleotide (NAD) to glu/asp residues on the substrate protein, and also polymerizes ADP-ribose to form long/branched chain polymers. PARP-1, one of 5 confirmed PARPs, is the most abundant and highly expressed enzyme.1 PARP-1 detects and relocates to single strand breaks or nicks in chromosomal DNA. PARP-1 is thought to play an important role in the initiation of the DNA repair pathway, although high levels of activation are also associated with increased apoptosis in response to genotoxic stress. In addition, PARP-1 may also operate downstream of the Raf-MEK-ERK pathway through direct interaction with ERK2 in the nucleus in a mechanism DNA damage.2 PARP inhibitors are being developed for use in a number of pathologies including cancer, diabetes, stroke and cardiovascular disease.
During cell death by apoptosis cleavage of PARP1 by caspases produces two fragments. The 25 kDa N-terminal peptide retains the DNA binding domains of PARP1. A C-terminal 85 kDa fragment has reduced catalytic activity and its detection is usually taken as a sensitive assay for apoptosis.3
REFERENCES
1. Tong, W.M. et al: Biochem. Biophy. Acta 1552:27-37, 2001
2. Kim, M.Y. et al: Gene Dev. 19:1951-1967, 2005
3. Garnier, P. et al: J. Neurosci. 23:7967-73, 2003
2. Kim, M.Y. et al: Gene Dev. 19:1951-1967, 2005
3. Garnier, P. et al: J. Neurosci. 23:7967-73, 2003
Products are for research use only. They are not intended for human, animal, or diagnostic applications.
(Click to Enlarge) Top: Left lane: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Right lane: Human cell line RT-4. Middle: Immunohistochemical staining of human cerebellum shows strong nuclear positivity in Purkinje cells. Bottom: Immunofluorescent staining of human cell line HEK 293 shows positivity in nucleus.
Details
Cat.No.: | CG1409 |
Antigen: | poly (ADP-ribose) polymerase 1 recombinant protein epitope signature tag (PrEST) |
Isotype: | Rabbit IgG |
Species & predicted species cross- reactivity ( ): | Human |
Applications & Suggested starting dilutions:* | WB 1:100-1:250 IP n/d IHC 1:1000-1:2500 (citrate retrieval) ICC n/d FACS n/d IF 1-4 µg/ml |
Predicted Molecular Weight of protein: | 113 kDa |
Specificity/Sensitivity: | Detects endogenous PARP-1 proteins without cross-reactivity with other family members. |
Storage: | Store at -20°C, 4°C for frequent use. Avoid repeated freeze-thaw cycles. |
*Optimal working dilutions must be determined by end user.
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Rabbit PARP-1 p116 Antibody: Rabbit PARP-1 p116 Antibody | Size: 100 ul | CAT.#: CG1409 | Price: $461.00 |