Instructions RCF
5 Important Cell Culture Rules
MSDS Cryopreserved Cells
Cell Apps Flyer Cardiovascular Cells
Cell Apps Poster Primary Cells
Cell Applications Inc Brochure
Description
Rat Cardiac Fibroblasts (RCF) are the most prevalent cell type in the rodent's heart, making up 60-70 % of all cells. They are known to play key roles in the development of cardiac disease and hypertrophy. Rat Cardiac Fibroblasts from Cell Applications, Inc. provide an excellent model system to study many aspects of heart function and pathophysiology, including the regulation of cell growth, survival, inflammation, hormone secretion and metabolism.
(Click to Enlarge) Rat Cardiac Fibroblasts: RCF. Isolated from rodent heart tissue.
Details
Tissue | Normal healthy rat heart |
QC | No bacteria, yeast, fungi, mycoplasma |
Bioassay | Attach, spread, proliferate in Growth Med |
Cryovial | 500,000 RCF (2nd passage) in frozen in Basal Medium w/ 10% FBS, 10% DMSO |
Kit | Cryovial frozen RCF (R306-05a/-05n), Gr Med (R116-500), Subcltr Rgnt Kit (090K) |
Proliferating | Shipped within 3 days after plating in Cltr Medium (flasks or plates) |
Doublings | At least 8 |
Applications | Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use. |
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Cryopreserved Rat Cardiac Fibroblast Total Kit, adult: 5x10^5 Cells (Adult), Medium & Subculture Reagents (See Details tab for specifics) | Size: 1 Kit | CAT.#: R306K-05a | Price: $807.00 | |
| Cryopreserved Rat Cardiac Fibroblasts (RCF), adult: Frozen RCF (5x10^5) | Size: 1 Cryovial | CAT.#: R306-05a | Price: $640.00 | |
| Proliferating Rat Cardiac Fibroblasts (RCF), adult: Actively growing, dividing cells in medium | Size: T-25 Flask | CAT.#: R307-25a | Price: $640.00 | |
| Proliferating Rat Cardiac Fibroblasts (RCF), adult: Actively growing, dividing cells in medium | Size: T-75 Flask | CAT.#: R307-75a | Price: $830.00 | |
| Proliferating Rat Cardiac Fibroblasts (RCF), adult: Actively growing, dividing cells in medium | Size: 24 Well | CAT.#: R307-24Wa | Price: $830.00 | |
| Proliferating Rat Cardiac Fibroblasts (RCF), adult: Actively growing, dividing cells in medium | Size: 96 Well | CAT.#: R307-96Wa | Price: $950.00 | |
| Cryopreserved Rat Cardiac Fibroblast Total Kit, neonatal: 5x10^5 Cells (Neonatal), Medium & Subculture Reagents (See Details tab for specifics) | Size: 1 Kit | CAT.#: R306K-05n | Price: $807.00 | |
| Cryopreserved Rat Cardiac Fibroblasts (RCF), neonatal: Frozen RCF (5x10^5) | Size: 1 Cryovial | CAT.#: R306-05n | Price: $640.00 | |
| Proliferating Rat Cardiac Fibroblasts (RCF), neonatal: Actively growing, dividing cells in medium | Size: T-25 Flask | CAT.#: R307-25n | Price: $640.00 | |
| Proliferating Rat Cardiac Fibroblasts (RCF), neonatal: Actively growing, dividing cells in medium | Size: T-75 Flask | CAT.#: R307-75n | Price: $830.00 | |
| Proliferating Rat Cardiac Fibroblasts (RCF), neonatal: Actively growing, dividing cells in medium | Size: 96 Well | CAT.#: R307-96Wn | Price: $950.00 | |
| Cryopreserved Rat Cardiac Fibroblasts (RCF), fetal: Frozen RCF (5x10^5) | Size: 1 Cryovial | CAT.#: R306-05f | Price: $662.00 | |
| Cryopreserved Rat Cardiac Fibroblast Total Kit, fetal: 5x10^5 Cells (Fetal), Medium & Subculture Reagents (See Details tab for specifics) | Size: 1 Kit | CAT.#: R306K-05f | Price: $829.00 | |
| Proliferating Rat Cardiac Fibroblasts (RCF), fetal: Actively growing, dividing cells in medium | Size: T-25 Flask | CAT.#: R307-25f | Price: $662.00 | |
| Proliferating Rat Cardiac Fibroblasts (RCF), fetal: Actively growing, dividing cells in medium | Size: 24 Well | CAT.#: R307-24Wf | Price: $852.00 | |
| Proliferating Rat Cardiac Fibroblasts (RCF), fetal: Actively growing, dividing cells in medium | Size: T-75 Flask | CAT.#: R307-75f | Price: $852.00 | |
| Proliferating Rat Cardiac Fibroblasts (RCF), fetal: Actively growing, dividing cells in medium | Size: 96 Well | CAT.#: R307-96Wf | Price: $972.00 |
Related Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Rat Fibroblast Basal Medium: Basal medium (contains no growth supplement). Add GS before use. | Size: 500 ml | CAT.#: R115-500 | Price: $74.00 | |
| Rat Fibroblast Growth Medium: All-in-one ready-to-use media | Size: 500 ml | CAT.#: R116-500 | Price: $114.00 | |
| Rat Fibroblast Growth Medium Kit: Basal medium & growth supplement sold together packaged separately | Size: Yields 500 ml | CAT.#: R116K-500 | Price: $124.00 | |
| Rat Fibroblast Growth Supplement: Added to Basal Medium to create Growth Medium | Size: 50 ml | CAT.#: R116-GS | Price: $66.00 | |
| Rat Fibroblast Basal Medium wo Phenol Red: Basal medium (contains no growth supplement). Add GS before use. Without Phenol Red. | Size: 500 ml | CAT.#: R115PR-500 | Price: $86.00 | |
| Rat Fibroblast Growth Medium wo FBS: Growth Medium without FBS | Size: 500 ml | CAT.#: R116F-500 | Price: $131.00 | |
| Rat Fibroblast Growth Supplement wo FBS: Growth Supplement without FBS | Size: 50 ml | CAT.#: R116F-GS | Price: $75.00 | |
| Rat Fibroblast Growth Medium wo Phenol Red: All-in-one ready-to-use media, without Phenol Red | Size: 500 ml | CAT.#: R116PR-500 | Price: $131.00 |
Extended Family Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Cyto-X Cell Counting Reagent Sample: 100 tests | Size: Sample | CAT.#: 028-S | Price: $41.00 | |
| Cyto-X Cell Counting Reagent: 500 tests | Size: 1 Bottle | CAT.#: 028-01 | Price: $160.00 | |
| Cytofect Fibroblast Transfection Kit (250 x 24-Wells): 250 x 24-Well Rxns | Size: 1 Kit | CAT.#: TF103K | Price: $465.00 | |
| Cytofect Fibroblast Transfection Sample Kit (25 x 24-Wells): 25 x 24-Well Rxns | Size: 1 Sample Kit | CAT.#: TF103KS | Price: $62.00 | |
| Rat Fibroblast Growth Factor-9 (FGF-9): Rat Fibroblast Growth Factor-9 | Size: 10 ug | CAT.#: RP3027-10 | Price: $194.00 | |
| Rat Fibroblast Growth Factor-9 (FGF-9): Rat Fibroblast Growth Factor-9 | Size: 100 ug | CAT.#: RP3027-100 | Price: $484.00 | |
| Rat Fibroblast Growth Factor-9 (FGF-9): Rat Fibroblast Growth Factor-9 | Size: 1000 ug | CAT.#: RP3027-1000 | Price: $4,090.00 | |
| Rat Cardiac Fibroblasts RNA (RCF RNA), Adult: Total RNA prepared from Rat Cardiac Fibroblasts | Size: 10 ug | CAT.#: R306-R10a | Price: $398.00 | |
| Rat Cardiac Fibroblasts RNA (RCF RNA), Adult: Total RNA prepared from Rat Cardiac Fibroblasts | Size: 25 ug | CAT.#: R306-R25a | Price: $796.00 | |
| Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution | Size: 1 Kit | CAT.#: 090K | Price: $63.00 |
Resources/Documents
Citations
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2015
Nakayama, A., H. Morita, T. Nakao, T. Yamaguchi, T. Sumida, Y. Ikeda, H. Kumagai, Y. Motozawa, T. Takahashi, A. Imaizumi, T. Hashimoto, R. Nagai, and I. Komuro. 2015. A Food-Derived Flavonoid Luteolin Protects against Angiotensin II-Induced Cardiac Remodeling. PLOS One, DOI: 10.1371/journal.pone.0137106.
2011
Hao, K., H. Hanawa, L. Ding, Y. Ota, K. Yoshida, K. Toba, M. Ogura, H. Ito, M. Kodama, and Y. Aizawa. 2011. Free heme is a danger signal inducing expression of proinflammatory proteins in cultured cells derived from normal rat hearts. Molec Immunol, 48:1191-1202.
Silberbach, M., C. Roberts, and N. Airhart. 2011. Methods of screening using a natriuretic peptide receptor. Patent US 7943296 B2.
Silberbach, M., C. Roberts, and N. Airhart. 2011. Natriuretic peptide related fragment in cardiovascular disease. Patent Application US 20110218230 A1.