Product Sheet CP10031
Description
BACKGROUND The S100 family comprises 16 closely related members. S100 proteins consist of two Ca2+-binding EF-hands and short individual N and C termini. The first EF-hand is conserved among the S100 family and the second EF-hand follows the canonical consensus. Almost all S100 proteins form stable homo- or heterodimers and several members have been shown to bind Zn2+ in addition to Ca2+ in vitro.1
Calcyclin, also called S100A6, is a member of the S100 family of calcium binding proteins and it also binds to Zn2+. Its gene was discovered on the basis of its cell cycle-dependent expression. This gene is expressed at its maximal level during the transition between G0 to S phase of the cell cycle. Later Calcyclin was found to be expressed at high levels in fibroblasts and epithelial cells, in cells with high proliferating activity, and those undergoing differentiation. Moreover, its expression is deregulated in in several tumors.2 Overexpression of S100A6 correlates with the metastatic behavior in nude mice and an up-regulated expression has also been observed in wound healing after corneal injury. To elucidate the function(s) of S100A6 recent efforts focused on the identification of cellular binding partners and the metal-binding characteristics. Several possible protein targets of Calcyclin have been identified. Calcyclin has been shown to interact in vitro in a Ca2+-dependent manner with glyceraldehyde-3-phosphate dehydrogenase, annexin II, annexin VI, annexin XI, caldesmon, and CacyBP (Calcyclin-binding protein).3
Calcyclin, also called S100A6, is a member of the S100 family of calcium binding proteins and it also binds to Zn2+. Its gene was discovered on the basis of its cell cycle-dependent expression. This gene is expressed at its maximal level during the transition between G0 to S phase of the cell cycle. Later Calcyclin was found to be expressed at high levels in fibroblasts and epithelial cells, in cells with high proliferating activity, and those undergoing differentiation. Moreover, its expression is deregulated in in several tumors.2 Overexpression of S100A6 correlates with the metastatic behavior in nude mice and an up-regulated expression has also been observed in wound healing after corneal injury. To elucidate the function(s) of S100A6 recent efforts focused on the identification of cellular binding partners and the metal-binding characteristics. Several possible protein targets of Calcyclin have been identified. Calcyclin has been shown to interact in vitro in a Ca2+-dependent manner with glyceraldehyde-3-phosphate dehydrogenase, annexin II, annexin VI, annexin XI, caldesmon, and CacyBP (Calcyclin-binding protein).3
REFERENCES
1. Zimmer, D.B. et al: Microsc. Res. Tech. 60:552-9, 2003
2. Rehman, I. et al: Br. J. Cancer91:739-44, 2004
3. Nowotny, M. et al: J. Biol. Chem. 275:31178-82, 2000
2. Rehman, I. et al: Br. J. Cancer91:739-44, 2004
3. Nowotny, M. et al: J. Biol. Chem. 275:31178-82, 2000
Products are for research use only. They are not intended for human, animal, or diagnostic applications.
(Click to Enlarge)
Top: Western Blot detection of Calcyclin proteins in bacterial lysate containing recombinant GST-Calcyclin fusion protein (39kDa) using Calcyclin Antibody. Bottom: This antibody stains paraffin-embedded human brain glioma tissue in immunohistochemical analysis.
Details
Cat.No.: | CP10031 |
Antigen: | Purified recombinant human Calcyclin protein fragments expressed in E. coli. |
Isotype: | Mouse IgG1 |
Species & predicted species cross- reactivity ( ): | Human, Mouse, Rat |
Applications & Suggested starting dilutions:* | WB 1:1000 IP 1:25 - 1:50 IHC 1:100 - 1:250 ICC n/d FACS n/d |
Predicted Molecular Weight of protein: | 12 kDa |
Specificity/Sensitivity: | Detects endogenous Calcyclin proteins in normal cell lysates without cross-reactivity with other family members. |
Storage: | Store at -20°C, 4°C for frequent use. Avoid repeated freeze-thaw cycles. |
*Optimal working dilutions must be determined by end user.
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Mouse Calcyclin Antibody: Mouse Calcyclin Antibody | Size: 100 ul | CAT.#: CP10031 | Price: $384.00 |