Description

The 3D Airway Model is a highly physiological, three-dimensional cellular system of Human Bronchial Epithelial Cells (HBEpC).  The model facilitates in vitro examination of epithelial function and disease, including airway infections, tissue repair mechanisms, signaling changes and potential treatments relevant to lung injuries.  Other investigations utilizing the Airway Model include mechanical and oxidative stress, inflammation, pulmonary diseases and smoking. The cells are grown and differentiated into a pseudostriated epithelium on PCF sitting inserts with a liquid/air interface. Contact us for more information.
(Click to Enlarge) A. Depiction of Human Airway.  B. Human Bronchial Epithelial Cell (HBEpC).  C. Crystal Violet Staining.  D. Hematoxylin & Eosin Staining of HBEpC differentiated into pseudostriated epithelium after 28 days.

Details

The Airway Model (Domestic US) consists of HBEpC differentiated into a pseudostriated epithelium, whereas the Total Kit (international customers) contains Cryopreserved HBEpC, media, twelve PCF inserts, reagents, subculture kit, and detailed instructions to form 3D Airway Model.

Products

Product Size CAT.# Price Quantity
3-D Airway Model : Cells, Media, Reagents & Inserts (See Details tab for specifics) Size: 1 Kit CAT.#: 502K-3D Price: $769.00
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3-D Airway Model (Not available for international orders): HBEpC differentiated into pseudostriated epithelium on PCF sitting inserts. Available only in the US. Approximate turnaround time 4-6 weeks. Size: 12 Sitting Inserts CAT.#: 502-3D-12 Price: $769.00
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3-D Airway Model (Not available for international orders): HBEpC differentiated into pseudostriated epithelium on PCF sitting inserts. Available only in the US. Approximate turnaround time 4-6 weeks. Size: 24 Sitting Inserts CAT.#: 502-3D-24 Price: $1,355.00
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Related Products

Product Size CAT.# Price Quantity
Cryopreserved Human Bronchial Epithelial Cell Total Kit, adult: 5x10^5 Cells (Adult), Medium & Subculture Reagents (See Details tab for specifics) Size: 1 Kit CAT.#: 502K-05a Price: $938.00
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Cryopreserved Bronchial Epithelial Cells (HBEpC), adult: Frozen HBEpC (5x10^5) Size: 1 Cryovial CAT.#: 502-05a Price: $740.00
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Bronchial Epithelial Cell RNA (HBEpC RNA), Adult: Total RNA prepared from Human Bronchial Epithelial Cells, adult Size: 10 ug CAT.#: 502-R10a Price: $398.00
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Bronchial Epithelial Cell RNA (HBEpC RNA), Adult: Total RNA prepared from Human Bronchial Epithelial Cells, adult Size: 25 ug CAT.#: 502-R25a Price: $796.00
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HBrEpC/HTrEpC Basal Medium: Without growth supplement (GS).  Add GS before use. Size: 500 ml CAT.#: 510-500 Price: $81.00
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HBEpC/HTEpC Differentiation Medium: Promotes cells to change from one type to another, more specialized Size: 250 ml CAT.#: 511D-250 Price: $148.00
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HBEpC/HTEpC Growth Medium: All-in-one ready-to-use. Cat# 511-500 fully supports cell growth on its own. Retinoic acid (RA) is a optional component; one vial of RA goes into 500ml media. Some researchers may elect to include RA if studies include cell differentiation or pseudo-stratification. Size: 500 ml CAT.#: 511-500 Price: $145.00
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HBEpC/HTEpC Growth Medium wo BPE: Growth medium without BPE Size: 500 ml CAT.#: 511B-500 Price: $144.00
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HBEpC/HTEpC Growth Medium wo BPE, Hydrocortisone, Insulin: Growth medium without BPE, hydrocortisone and insulin Size: 500 ml CAT.#: 511BHI-500 Price: $144.00
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HBEpC/HTEpC Growth Medium wo T3: Growth medium without Tri-iodothyronine (T3) Size: 500 ml CAT.#: 511T3-500 Price: $144.00
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HBEpC/HTEpC Growth Medium wo Antibiotics: Growth medium without antibiotics Size: 500 ml CAT.#: 511A-500 Price: $144.00
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HBEpC/HTEpC Growth Medium Kit: Basal medium & growth supplement sold together packaged separately Size: 1 Kit CAT.#: 511K-500 Price: $154.00
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HBEpC/HTEpC Growth Supplement: Added to Basal Medium to create Growth Medium Size: 5 ml CAT.#: 511-GS Price: $81.00
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Extended Family Products

Product Size CAT.# Price Quantity
HBSS: 1X HBSS for washing of cells before Trypsin/EDTA treatment Size: 100 ml CAT.#: 062-100 Price: $23.00
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Trypsin/EDTA Solution: For subculturing of adherent cells. Size: 100 ml CAT.#: 070-100 Price: $23.00
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Trypsin Neutralization Solution: Stops the trypsinization reaction. Size: 100 ml CAT.#: 080-100 Price: $23.00
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Cyto-X Cell Counting Reagent Sample: 100 tests Size: Sample CAT.#: 028-S Price: $41.00
Cyto-X Cell Counting Reagent: 500 tests Size: 1 Bottle CAT.#: 028-01 Price: $160.00

Publications

2009
Song, H., H. Wan, Y. Araya and D. Perez. 2009. Partial direct contact transmission in ferrets of a mallard H7N3 influenza virus with typical avian-like receptor specificity. 2009. Virology Journal, 6:126.
2007
Wan, H. and D. Perez.  2007.  Amino Acid 226 in the Hemagglutinin of H9N2 Influenza Viruses Determines Cell Tropism and Replication in Human Airway Epithelial Cells. J Virol, 81:5181-5191.