Description
The 3D Skin Model is a highly physiological, three-dimensional cellular system of Human Epidermal Keratinocytes (HEK) for in vitro studies, offering an excellent tool to examine aspects of epithelial function and disease, particularly those related to skin biology and toxicology.
Basic science and pharmaceutical researchers use the 3D Skin Model to replace animal tests for the assessment of skin irritation due to chemicals, skin-cream-based drugs and cosmetics. CAI's Skin Model can also help avoid misclassification of chemicals and skin corrosion observed in animal systems (1, 2). Other applications include phototoxicity, percutaneous absorption & penetration, wound healing and metabolism. The cells are grown and differentiated into a stratified squamous epithelium on PCF sitting inserts with a liquid/air interface. Contact us for more information.
- Eun & Nam, Exog Dermatol, 2:1–5 (2003)
- York et al., Contact Dermatitis, 34:204 (1996)
(Click to Enlarge) A. Depiction of Human Skin. B. Human Epidermal Keratinocytes (HEK) at ~80% confluency. C. Crystal Violet Staining. D. Hematoxylin & Eosin Staining of HEK differentiated into stratified squamous epithelium after 14 days.
Details
The Skin Model (domestic US) consists of HEK differentiated into stratified squamous epithelium, whereas the Kit (for international customers) contains Cryopreserved HEK, media, PCF inserts, reagents, subculture kit, and detailed instructions to form 3D Skin Model.
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| 3-D Skin Model: Cells, Media, Reagents & Inserts (See Details tab for specifics) | Size: 1 Kit | CAT.#: 102K-3D | Price: $640.00 | |
| 3-D Skin Model: HEK differentiated into stratified squamous epithelium on PCF sitting inserts. Available only in the US. Approximate turnaround time 3-5 weeks. | Size: 12 Sitting Inserts | CAT.#: 102-3D-12 | Price: $640.00 | |
| 3-D Skin Model (Not available for international orders): HEK differentiated into stratified squamous epithelium on PCF sitting inserts. Available only in the US. Approximate turnaround time 3-5 weeks. | Size: 24 Sitting Inserts | CAT.#: 102-3D-24 | Price: $1,216.00 |
Related Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Cryopreserved Human Epidermal Keratinocytes Total Kit, fetal: 5x10^5 Cells (Fetal), Medium & Subculture Reagents (See Details tab for specifics) | Size: 1 Kit | CAT.#: 102K-05f | Price: $646.00 | |
| Cryopreserved Epidermal Keratinocytes (HEK), fetal: Frozen HEK (5x10^5) | Size: 1 Cryovial | CAT.#: 102-05f | Price: $475.00 | |
| Cryopreserved Human Epidermal Keratinocytes Total Kit, neonatal: 5x10^5 Cells (Neonatal), Medium & Subculture Reagents (See Details tab for specifics) | Size: 1 Kit | CAT.#: 102K-05n | Price: $646.00 | |
| Cryopreserved Epidermal Keratinocytes (HEK), neonatal: Frozen HEK (5x10^5) | Size: 1 Cryovial | CAT.#: 102-05n | Price: $475.00 | |
| Epidermal Keratinocyte RNA (HEK RNA), Neonatal: Total RNA prepared from Human Epidermal Keratinocytes, neonatal | Size: 25 ug | CAT.#: 102-R25n | Price: $796.00 | |
| Epidermal Keratinocyte RNA (HEK RNA), Neonatal: Total RNA prepared from Human Epidermal Keratinocytes, neonatal | Size: 10 ug | CAT.#: 102-R10n | Price: $398.00 | |
| Human EpiVita Basal Medium: Basal medium (contains no growth supplement). Add GS before use. | Size: 500 ml | CAT.#: 140-500 | Price: $96.00 | |
| Human EpiVita Defined Growth Medium: All-in-one ready-to-use | Size: 500 | CAT.#: 141DGM-500 | Price: $121.00 | |
| Human EpiVita Defined Growth Medium Kit: Basal medium & growth supplement sold together packaged separately | Size: Yields 500 ml | CAT.#: 141DGMK-500 | Price: $133.00 | |
| Human EpiVita Defined Growth Supplement: Added to Basal Medium to create Growth Medium | Size: 5 ml | CAT.#: 141DGM-GS | Price: $61.00 | |
| Human EpiVita Serum-Free Growth Medium for Neonatal Cells: All-in-one ready-to-use, standard Epithelial Cell Medium, for neonatal and fetal cells. Does not contain FBS. | Size: 500 | CAT.#: 141-500 | Price: $118.00 | |
| Human EpiVita Serum-Free Growth Medium for Adult Cells: All-in-one ready-to-use, standard Epithelial Cell Medium, for adult cells. Does not contain FBS. | Size: 500 ml | CAT.#: 141-500a | Price: $121.00 | |
| Human EpiVita Serum-Free Growth Medium Kit for Neonatal Cells: Basal medium & growth supplement sold together packaged separately, for neonatal and fetal cells. Does not contain FBS. | Size: Yields 500 ml | CAT.#: 141K-500 | Price: $131.00 | |
| Human EpiVita Serum-Free Growth Medium Kit for Adult Cells: Basal medium & growth supplement sold together packaged separately, for adult cells. Does not contain FBS. | Size: Yields 500 ml | CAT.#: 141K-500a | Price: $133.00 | |
| Human EpiVita Serum-Free Growth Supplement for Neonatal Cells: Added to Basal Medium to create Growth Medium, for neonatal and fetal cells. Does not contain FBS. | Size: 5 ml | CAT.#: 141-GS | Price: $59.00 | |
| Human EpiVita Serum-Free Growth Supplement for Adult Cells: Added to Basal Medium to create Growth Medium, for adult cells. Does not contain FBS. | Size: 5 ml | CAT.#: 141-GSa | Price: $61.00 |
Extended Family Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Cyto-X Cell Counting Reagent Sample: 100 tests | Size: Sample | CAT.#: 028-S | Price: $41.00 | |
| Cyto-X Cell Counting Reagent: 500 tests | Size: 1 Bottle | CAT.#: 028-01 | Price: $160.00 | |
| HBSS: 1X HBSS for washing of cells before Trypsin/EDTA treatment | Size: 100 ml | CAT.#: 062-100 | Price: $23.00 | |
| Trypsin/EDTA Solution: For subculturing of adherent cells. | Size: 100 ml | CAT.#: 070-100 | Price: $23.00 | |
| Trypsin Neutralization Solution: Stops the trypsinization reaction. | Size: 100 ml | CAT.#: 080-100 | Price: $23.00 |
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