Activin A: Rat Activin A

Cat. No.:

RP3006 / RP3006AF

 

Alternate Names:

Inhibin beta-1, FRP, FSH-releasing protein

 

 

Source:

E. coli

 

Physical Appearance:

Sterile Filtered white lyophilized (freeze-dried) powder.

 

Formulation:

0.1% Trifluoroacetic Acid (TFA)

 

Stability:

Lyophilized product is very stable at -20°C. Reconstituted material should be aliquoted and frozen at -20°C. It is recommended that a carrier protein (0.1% HSA or BSA) is added for long term storage.

 

Reconstitution:

Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/mL, which can be further diluted into other aqueous solutions.

 

Protein Content and Purity determined by:

• UV spectroscopy at 280 nm.

• RP-HPLC calibrated against a known standard.

• Quantitation against a known standard via reducing and non-reducing SDS-PAGE gels.

 

Endotoxin Level:

Endotoxin level, as measured by LAL analysis, is <0.01ng/ug or <0.1EU/ug.

 

Biological Activity:

The activity is measured by dose-dependent cytotoxicity of MPC-11 cells, with Bioactivity Acceptance Criteria ED50 at ng/mL.

 

 

Animal Component-Free

This product is produced with no animal derived raw products.  All processing and handling employs animal free equipment and animal free protocols.