Product Sheet CP10001
Description
BACKGROUND 4E-BP1 (also called PHAS-I, eIF4E-binding protein 1) is one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5\' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.1,2 It mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.3
REFERENCES
1. Gingras, A.C. et al: Gene Dev. 12:502-13, 1998
2. Yamaguchi, S. et al: Cell Metabol. 7:269-276, 2008
3. Gingras, A.C. et al: Gene Dev. 13:1422-1437, 1999
2. Yamaguchi, S. et al: Cell Metabol. 7:269-276, 2008
3. Gingras, A.C. et al: Gene Dev. 13:1422-1437, 1999
Products are for research use only. They are not intended for human, animal, or diagnostic applications.
(Click to Enlarge)
Top: Western Blot detection of 4E-BP1 proteins in A431 cell lysate using 4E-BP1 Antibody. Bottom: This antibody stains paraffin-embedded human ovary carcinoma tissue in immunohistochemical analysis.
Details
Cat.No.: | CP10001 |
Antigen: | Purified recombinant human 4E-BP1 expressed in E. coli. |
Isotype: | Mouse IgG1 |
Species & predicted species cross- reactivity ( ): | Human, Mouse, Rat |
Applications & Suggested starting dilutions:* | WB 1:1000 IP n/d IHC 1:200 ICC n/d FACS n/d |
Predicted Molecular Weight of protein: | 15-20 kDa |
Specificity/Sensitivity: | Detects endogenous levels of 4E-BP1 proteins without cross-reactivity with other family members. |
Storage: | Store at -20°C, 4°C for frequent use. Avoid repeated freeze-thaw cycles. |
*Optimal working dilutions must be determined by end user.
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Mouse Monoclonal 4E-BP1 Antibody: Mouse Monoclonal 4E-BP1 Antibody | Size: 100 ul | CAT.#: CP10001 | Price: $333.00 |