Product Sheet CA1391

Description

BACKGROUND BAFF (B cell activating factor), a member of the TNF family (also termed TALL-1, THANK, BlyS and zTNF4) and BAFF receptor (BAFF-R) play a fundamental role during the transition from immature T1 to T2 B cells and therefore for the generation of mature B cells in the spleen. This was clearly demonstrated by an almost complete lack of follicular and marginal zone B cells and by a block at the T1 cell stage in BAFF as well as in BAFF-R deficient mice. In these mice, the B-1 compartment was not affected, indicating that the development of this subset was independent of BAFF-BAFF-R signaling. On the other hand, transgenic mice over-expressing BAFF display an overall increase in all B cell subsets, suggesting that all mature B cells express BAFF-R on their surface or are able to respond to BAFF.1

BAFF-R (also called BR3) is the most unique of the 3 tumor necrosis factor receptors (TNFRs) for BLyS (B-lymphocyte stimulator; also called BAFF). A/WySNJ mice (which have a mutant BAFF-R gene) have a low peripheral blood B-cell fraction that is similar to that seen in BLyS-deficient mice, suggesting that BAFF-R transmits critical B-cell survival signals associated with BLyS stimulation. Downstream mediators of BAFF-R activation include both the canonical (classic, NF-κB1) and alternative (noncanonical, NF-κB2) NF-κB pathways. Although BLyS/BAFF-R–derived intracellular signaling pathways are still incompletely defined, this ligand/receptor dyad provides key regulatory control of antiapoptotic cell survival and growth stimulation. In this regard, BLyS modulates several antiapoptotic Bcl-2 family members, including Bcl-xL, Mcl-1, A-1, Bcl-2, and Bim, via survival-promoting kinase systems such as Pim 1/2 or Erk as well as proteins involved in early cell-cycle progression, including c-myc, p27Kip1, cyclin D1, and cyclin D2. The binding of BAFF to the BAFF-R leads to the activation of the NF-κB pathway and ultimately to the transcription of the anti-apoptotic factor Bcl-2. The finding that Bcl-2 over-expression can, to a large extent, rescue the mature B cell compartment in BAFF signaling deficient mice, indicates that Bcl-2 expression induced by BAFF is crucial for the survival of B cells during the transition from immature to mature stages. Moreover, BAFF-BAFF-R signaling was also playing a central role in the in vivo maintenance of the peripheral mature B cell pool.2 Furthermore, BAFF-R was found to be presented in the cell nucleus as well as in the plasma membrane and cytoplasm, in both normal peripheral blood B lymphocytes and aggressive NHL-B cells. It demonstrated that in addition to activating the NF-κB pathways in the plasma membrane, BAFF-R can also promote normal and NHL-B-cell survival and proliferation by directly functioning as a transcriptional cofactor with other NF-κB transcription factor(s) and possibly regulating transcription of other NF-κB target genes.3

 
REFERENCES  
1. Mackay, F. & Schneider, P.: Nature Rev. Immunol. 9:491-502, 2009
2. Rauch, M. et al: PloS ONE 4:e5456, 2009
3. Fu, L. et al:Blood 113:4627-36, 2009
  
Products are for research use only. They are not intended for human, animal, or diagnostic applications.

(Click to Enlarge) Western Blot detection of recombinant human BAFF-R cytoplasmic domain fragments using BAFF-R Antibody.

Details

Cat.No.:
CA1391
Antigen:
A short peptide from human BAFF-R sequence.
Isotype:
Rabbit IgG
Species & predicted
species cross-
reactivity ( ):
Human
Applications &
Suggested starting
dilutions:*
WB                  1:500 - 1:1000
IP                    n/d
IHC                  1:50 - 1:100
ICC                  n/d
FACS               n/d
Predicted Molecular
Weight of protein:
25 kDa
Specificity/Sensitivity:
Detects endogenous BAFF-R proteins without cross-reactivity with other family members.
Storage:
Store at -20°C, 4°C for frequent use. Avoid repeated freeze-thaw cycles.

*Optimal working dilutions must be determined by end user.

Products

Product Size CAT.# Price Quantity
Rabbit B Cell Activating Factor Receptor Anitbody: Rabbit B Cell Activating Factor Receptor Anitbody Size: 100 ul CAT.#: CA1391 Price: $302.00
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