Product Sheet CB12856
Description
BACKGROUND c-Kit is a receptor tyrosine kinase that binds stem cell factor (SCF). Structurally, c-Kit contains five immunoglobulin-like domains extracellularly and a catalytic domain divided into two regions by a 77 amino acid insert intracellularly. c-Kit is expressed in hematopoietic stem and progenitor cells and in several non-hematopoietic tissues. Moreover, human and mouse embryonic stem cells express Kit transcripts.1 Signaling by SCF and Kit, its receptor, plays important roles in gametogenesis, hematopoiesis, mast cell development and function, and melanogenesis. Downstream of c-Kit, multiple signal transduction components are activated, including phosphatidylinositol-3-kinase, Src family members, the JAK/STAT pathway and the Ras-Raf-MAP kinase cascade.2 c-Kit gene mutations and overexpression have been shown in a number of different neoplasms, particularly in mastocytosis and gastrointestinal stromal tumours (GIST). c-Kit is a drug target for these tumor treatments. Glevec has been successfully used for this purpose.3
REFERENCES
1. Edling, C.E. & Hallberg, B.: Int. J. Biochem, cell Biol. 39:1995-8, 2007
2. Loveland, K. L. & Schlatt, S.: J. Endocrinol. 153: 337-44, 1997.
3. Miettinen, M. & Lasota, J.: Arch. Path. Lab. Med. 130: 1466-78, 2006.
2. Loveland, K. L. & Schlatt, S.: J. Endocrinol. 153: 337-44, 1997.
3. Miettinen, M. & Lasota, J.: Arch. Path. Lab. Med. 130: 1466-78, 2006.
Products are for research use only. They are not intended for human, animal, or diagnostic applications.
(Click to Enlarge) Specific detection of c-Kit proteins in MO7e cell lysates by Western blot analysis using c-Kit Monoclonal Antibody (8H18).
Details
Cat.No.: | CB12856 |
Antigen: | NSO-expressed recombinant human c-Kit protein |
Isotype: | Mouse monoclonal IgG1 |
Species & predicted species cross- reactivity ( ): | Human, Mouse |
Applications & Suggested starting dilutions:* | WB 1:1000 IP n/d IHC (Paraffin) n/d ICC n/d FACS n/d |
Predicted Molecular Weight of protein: | 145 kDa |
Specificity/Sensitivity: | Does not cross-react with other PDGFR family members |
Storage: | -20° |
*Optimal working dilutions must be determined by end user.
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Monoclonal Anti-c-Kit Antibody: Monoclonal Anti-c-Kit Antibody | Size: 100 ul | CAT.#: CB12856 | Price: $302.00 |