Product Sheet CA1020
Description
BACKGROUND CD40L (also called CD154) is a member of the tumor necrosis factor family of ligands, plays a major role in immune responses via its receptor, CD40.1 CD40L is mainly expressed on activated CD4+ T lymphocytes. Recently, CD40L has been detected on the surfaces of activated platelets and shown to activate endothelium. It was also shown that HUVEC cell express CD40L.2 Whereas CD40, a member of the tumor necrosis factor receptor (TNFR) family, has been found on the surface of B lymphocytes, monocytes, hematopoietic progenitors, dendritic cells (DCs), endothelial cells, epithelial cells and so on. CD40L-CD40 interaction plays important role in control of thymus-dependent humoral immunity and cell mediated immune responses. CD40L is the most crucial molecule for activating antigen-presenting cells (APCs) and thereby initiating the immune response.3 Deletion or mutations of the CD40L molecule leads to severe immunodeficiency, in both man and mouse, characterized by hypogamma-globulinemia as well as T cell defects in cell mediated immunity.4 Moreover, blockade of the CD40L/CD40 pathway by antibodies to CD40L has been used, in experimental animals,5 to effectively prevent and treat a variety of autoimmune diseases as well as allograft rejection without inducing generalized immune suppression.
REFERENCES
1. Daoussis D et al.: Clin. Diagn. Lab. Immunol. 11:635-641, 2004.
2. Mendez-Cruz AR et al.: Immunol. Lett. 111:116-123, 2007.
3. Boisvert J et al.: J. Immunol. 173: 3647-3652, 2004.
4. Weller S et al.: Proc. Natl. Acad. Sci. USA 98:1166-1170, 2001.
5. Lutgens E et al.: Proc. Natl. Acad. Sci. USA 97:7564-7469, 2000.
2. Mendez-Cruz AR et al.: Immunol. Lett. 111:116-123, 2007.
3. Boisvert J et al.: J. Immunol. 173: 3647-3652, 2004.
4. Weller S et al.: Proc. Natl. Acad. Sci. USA 98:1166-1170, 2001.
5. Lutgens E et al.: Proc. Natl. Acad. Sci. USA 97:7564-7469, 2000.
Products are for research use only. They are not intended for human, animal, or diagnostic applications.
(Click to Enlarge) Western Blot detection of endogenous CD40L protein from Human Umbilical Vein Endothelial Cell, HUVEC, lysate using CD40L Antibody.
Details
Cat.No.: | CA1020 |
Antigen: | Human CD40L carboxyl-terminal sequence |
Isotype: | Rabbit Polyclonal |
Species & predicted species cross- reactivity ( ): | Human, Rat, Mouse |
Applications & Suggested starting dilutions: | WB 1:1000 IP 1:50 IHC (Paraffin) 1:100 ICC n/d FACS n/d |
Predicted Molecular Weight of protein: | 30 kDa |
Specificity/Sensitivity: | Detects endogenous levels of CD40L protein in normal primary cell lysates. |
Storage: | Store at 4° C for frequent use; at -20° C for at least one year. |
*Optimal working dilutions must be determined by end user.
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Polyclonal CD40 Ligand Antibody: Polyclonal CD40 Ligand Antibody | Size: 100 ul | CAT.#: CA1020 | Price: $302.00 |