Description
BACKGROUND CDC2/CDK1 is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control.1 It is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II.2 Alternatively spliced transcript variants encoding different isoforms have been found for CDC2.
One important mechanism regulating the activity of CDC2 during the cell cycle is based on phosphorylation/ dephosphorylation. Avian CDC2 is phosphorylated Thr14, Tyr15, Thr161 and Ser277. Dephosphorylation of both Thr14 and Tyr15 is required for activation of CDC2 at the G2/M transition, indicating that phosphorylation of these residues negatively regulates CDC2 activity. Conversely, phosphorylation of Thr161 is required for kinase activity.3
One important mechanism regulating the activity of CDC2 during the cell cycle is based on phosphorylation/ dephosphorylation. Avian CDC2 is phosphorylated Thr14, Tyr15, Thr161 and Ser277. Dephosphorylation of both Thr14 and Tyr15 is required for activation of CDC2 at the G2/M transition, indicating that phosphorylation of these residues negatively regulates CDC2 activity. Conversely, phosphorylation of Thr161 is required for kinase activity.3
REFERENCES
1. Yasuda,H. et al: Yakugaku Zasshi 113:829-46, 1993
2. Cisek, S.J. & Gorden, J.L. : Nature 339:679-84, 1989
3. Nigg, E.A. et al:Ciba Found. Symp. 170:72-84, 1992
2. Cisek, S.J. & Gorden, J.L. : Nature 339:679-84, 1989
3. Nigg, E.A. et al:Ciba Found. Symp. 170:72-84, 1992
Products are for research use only. They are not intended for human, animal, or diagnostic applications.
Top: Western Blot detection of CDC2/CDK1 proteins in Jurkat cell lysate using CDC2/CDK1 Antibody. Middle: This antibody stains HeLa cells in confocal immunofluorescent analysis (CDC2/CDK1 Antibody: Green; Actin filaments: Red; DRAQ5 DNA Dye: Blue).
Bottom: It also specifically reacts with CDC2/CDK1 proteins in PC-2 cells by FACS testing (CDC2/CDK1 Antibody: Green; control; Purple).
Details
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Cat.No.:
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CP10295
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Antigen:
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Raised against recombinant human CDC2/CDK1 fragments expressed in E. coli.
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Isotype:
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Mouse IgG1
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Species & predicted
species cross-
reactivity ( ):
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Human, Mouse, Rat
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Applications &
Suggested starting
dilutions:*
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WB 1:1000
IP n/d
IHC n/d
ICC 1:50 - 1:200
FACS 1:50 - 1:200
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Predicted Molecular
Weight of protein:
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34 kDa
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Specificity/Sensitivity:
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Detects endogenous CDC2/CDK1 proteins without cross-reactivity with other family members.
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Storage:
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Store at -20°C, 4°C for frequent use. Avoid repeated freeze-thaw cycles.
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*Optimal working dilutions must be determined by end user.
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Mouse CDC2/CDK1 Antibody: Mouse CDC2/CDK1 Antibody | Size: 100 ul | CAT.#: CP10295 | Price: $333.00 |