Product Sheet CP10067
Description
BACKGROUND The death domain-associated protein (Daxx) is a multifunctional protein. It exerts many reported functions that include mediating the signaling from FasL to apoptosis via activating the c-Jun N-terminal kinase (JNK) induction and inhibition of apoptosis, and regulation of chromatin remodeling. Daxx resides in multiple locations in the nucleus and in the cytoplasm. It was originally cloned as a CD95 (FAS)-interacting protein and modulator of FAS-induced cell death.1 It interacts with a wide variety of proteins, such as apoptosis antigen Fas, centromere protein C, and as HDAC1, HDAC2, androgen receptor (AR), Smad4, p53, paired box gene 3 (Pax3), and Axin. In the nucleus, Daxx functions as a potent transcription repressor that binds to sumoylated transcription factors. Its repression can be relieved by the sequestration of this protein into promyelocytic leukemia nuclear bodies or nucleoli.2 This protein also associates with centromeres in G2 phase. In the cytoplasm, the encoded protein may function to regulate apoptosis. The subcellular localization and function of this protein are modulated by post-translational modifications, including sumoylation, phosphorylation and polyubiquitination.3 Alternative splicing results in multiple transcript variants.
REFERENCES
1. Salomoni, P. & Khelifi, A.F.: Trends Cell Biol. 16:97-104, 2006
2. Chen, S.F. et al: Ai Zheng 28:1333-6, 2009
3. Lin, S.C. & Li, Q.: Cell Res. 17:301-2, 2007
2. Chen, S.F. et al: Ai Zheng 28:1333-6, 2009
3. Lin, S.C. & Li, Q.: Cell Res. 17:301-2, 2007
Products are for research use only. They are not intended for human, animal, or diagnostic applications.
(Click to Enlarge)
Top: Western Blot detection of Daxx proteins in K562 using Daxx Antibody. Middle: This antibody stains Panc-1 cells in confocal immunofluorescent testing. Bottom: It also detects Daxx proteins (Green) vs. negative control mouse IgG (Purple) in HeLa cells in FACS analysis.
Details
Cat.No.: | CP10067 |
Antigen: | Purified recombinant human Daxx fragments expressed in E. coli. |
Isotype: | Mouse IgG1 |
Species & predicted species cross- reactivity ( ): | Human |
Applications & Suggested starting dilutions:* | WB 1:1000 IP n/d IHC n/d ICC 1:200 FACS 1:200 |
Predicted Molecular Weight of protein: | 110 kDa |
Specificity/Sensitivity: | Detects endogenous Daxx proteins without cross-reactivity with other family members. |
Storage: | Store at -20°C, 4°C for frequent use. Avoid repeated freeze-thaw cycles. |
*Optimal working dilutions must be determined by end user.
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Mouse Daxx Antibody: Mouse Daxx Antibody | Size: 100 ul | CAT.#: CP10067 | Price: $333.00 |