Product Sheet CP10072

Description

BACKGROUND Dickkopf (DKK) family proteins, including DKK1–4, are a group of secreted glycoproteins and antagonists of Wnt signaling. DKK1, DKK2 and DKK4 interact with Wnt coreceptors low-density Lipoprotein receptor-related protein 5/6, which disrupt their bindings to the Wnt ligands.1 DKK family members, in particular DKK3, were shown to be downregulated in a variety of tumor cells, such as those from hepatocellular carcinoma, lymphoblastic leukemia, prostate cancer, renal cell carcinoma and melanoma. Hypermethylation of DKK1 and DKK3 promoters was detected in several types of tumors.2 However, the physiological relevance of altered expression of DKK family proteins in tumor formation remains unclear. Furthermore, the functional role of DKK3 in Wnt/b-catenin signaling has been obscure. While earlier studies suggested that DKK3 does not affect Wnt signaling, recent studies demonstrated that DKK3 can reduce cytoplasmic accumulation of beta-catenin in Saos-2 osteosarcoma cells and inhibit TCF-4 activity in PC12 rat pheochromocytoma cells. It was demonstrated that DKK3 can suppress lung cancer cell growth, probably through inhibiting b-catenin/TCF-4 signaling by preventing beta-catenin nuclear translocation.3
 
REFERENCES  
1. Glinka, A. et al: Nature 391;357-62, 1998
2. Mao, B. et al: Nature 417:664-7, 2002
3. Yue, W. et al: Carcinogen. 29:84-92, 2008
  
Products are for research use only. They are not intended for human, animal, or diagnostic applications.
(Click to Enlarge)
Top: Western Blot detection of DKK3 proteins in various cell lysates using DKK3 Antibody. Bottom: This antibody also specifically reacts with DKK3 protein in MCF-7 cells (Green) vs. normal mouse IgG control (Purple) in FACS analysis.

Details

Cat.No.:
CP10072
Antigen:
Purified recombinant human DKK3 fragments expressed in E. coli.
Isotype:
Mouse IgG1
Species & predicted
species cross-
reactivity ( ):
Human, Monkey
Applications &
Suggested starting
dilutions:*
WB                  1:1000
IP                    n/d
IHC                  n/d
ICC                  n/d 
FACS               1:200
Predicted Molecular
Weight of protein:
38 kDa
Specificity/Sensitivity:
Detects endogenous DKK3 proteins without cross-reactivity with other family members.
Storage:
Store at -20°C, 4°C for frequent use. Avoid repeated freeze-thaw cycles.

*Optimal working dilutions must be determined by end user.

Products

Product Size CAT.# Price Quantity
Mouse Dickkopf 3 Antibody: Mouse Dickkopf 3 Antibody Size: 100 ul CAT.#: CP10072 Price: $384.00
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Resources/Documents