Product Sheet CB13452
Description
BACKGROUND Elk1 is a member of ETS-domain transcription factor family. ETS proteins form one of the largest families of signal-dependent transcriptional regulators, mediating cellular proliferation, differentiation and tumorigenesis.1 Elk-1 is regulated by phosphorylation in response to activation of the MAPK (mitogen-activated protein kinase) pathways. This phosphorylation triggers a series of molecular events that convert Elk-1 from a transcriptionally silent state into a highly active state and then back to a basal level.2 At the same time, activation of the ERK (extracellular-signal-regulated kinase) MAPK pathway leads to loss of modification of Elk-1 by SUMO (small ubiquitin-related modifier). As SUMO imparts repressive properties on Elk-1, ERK-mediated SUMO loss leads to de-repression at the same time as the ERK pathway promotes activation of Elk-1.3 Thus a two-step mechanism is employed to convert Elk-1 into its fully activated state.
REFERENCES
1. Tsu, T. et al.: J. Cell. Biochem. 91:896, 2004.
2. Yang, S. H. & Sharrocks, A. D.: Biochem. Soc. Symp. 73:121, 2006.
3. Yang, S. H. et al.: Mol. Cell 12:63, 2003.
2. Yang, S. H. & Sharrocks, A. D.: Biochem. Soc. Symp. 73:121, 2006.
3. Yang, S. H. et al.: Mol. Cell 12:63, 2003.
Products are for research use only. They are not intended for human, animal, or diagnostic applications.
(Click to Enlarge) Western blot detection of Elk1 protein K562 cell lysate using Elk1 Antibody.
Details
Cat.No.: | CB13452 |
Antigen: | E. coli-expressed recombinant human Elk1 protein. |
Isotype: | Mouse Monoclonal IgG1 |
Species & predicted species cross- reactivity ( ): | Human, Mouse, Rat |
Applications & Suggested starting dilutions:* | WB 1:1000 IP n/d IHC n/d ICC n/d FACS n/d |
Predicted Molecular Weight of protein: | 62 kDa |
Specificity/Sensitivity: | Detects Elk1 protein and does not cross-react with related proteins. |
Storage: | Store at -20°C, 4°C for frequent use. Avoid repeated freeze-thaw cycles. |
*Optimal working dilutions must be determined by end user.
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Mouse Elk1 Antibody: Mouse Elk1 Antibody | Size: 200 ul | CAT.#: CB13452 | Price: $302.00 |