Product Sheet CP10101
Description
BACKGROUND Fibrinogen is a plasma glycoprotein mainly synthesized by hepatocytes that circulates as a 340-kDa hexamer consisting of pairs of Aalpha, Bbeta, and gammachains. Its 3-dimensional structure is characterized by 2 lateral nodules (each composed of 2 globular subdomains) joined to a central nodule by coiled-coil triple-helix structures. The Aalpha, Bbeta, and gamma chains are encoded by 3 different genes (FGA, FGB, and FGG, respectively) clustered on chromosome 4q28. Fibrinogen takes part in hemostasis by forming the fibrin clot and by mediating platelet aggregation.1 Moreover; it is a class II acute-phase reacting protein involved in the acute phase response to injury and stress.2
FGB (fibrinogen beta chain) is the beta component of fibrinogen. Following vascular injury, fibrinogen is cleaved by thrombin to form fibrin which is the most abundant component of blood clots. In addition, various cleavage products of fibrinogen and fibrin regulate cell adhesion and spreading, display vasoconstrictor and chemotactic activities, and are mitogens for several cell types. Mutations in FGB gene lead to several disorders, including afibrinogenemia, dysfibrinogenemia, hypodysfibrinogenemia and thrombotic tendency.3
REFERENCES
1. Doolittle, R.F. et al: Ann. Rev. Biochem. 53:195-229, 1984
2. Kushner, I.: Method Enzymol. 163:373-83, 1988
3. Mammen, E.F.:Semin Thromb Hemost. 9:1-72, 1983
2. Kushner, I.: Method Enzymol. 163:373-83, 1988
3. Mammen, E.F.:Semin Thromb Hemost. 9:1-72, 1983
Products are for research use only. They are not intended for human, animal, or diagnostic applications.
(Click to Enlarge) Western Blot detection of FGB proteins in human plasma using FGB Antibody.
Details
Cat.No.: | CP10101 |
Antigen: | Purified recombinant human FGB fragments expressed in E. coli. |
Isotype: | Mouse IgG |
Species & predicted species cross- reactivity ( ): | Human, Mouse, Rat |
Applications & Suggested starting dilutions:* | WB 1:1000 IB 1:50 IHC n/d ICC n/d FACS n/d |
Predicted Molecular Weight of protein: | 52 kDa |
Specificity/Sensitivity: | Detects endogenous FGB proteins without cross-reactivity with other family members. |
Storage: | Store at -20°C, 4°C for frequent use. Avoid repeated freeze-thaw cycles. |
*Optimal working dilutions must be determined by end user.
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Mouse Fibrogen Beta Chain Antibody: Mouse Fibrogen Beta Chain Antibody | Size: 100 ul | CAT.#: CP10101 | Price: $413.00 |