Product Sheet CP10120
Description
BACKGROUND GST is commonly used to create fusion proteins.1 The tag has the size of 220 amino acids (roughly 26 KDa), which, compared to other tags like the myc- or the FLAG-tag, is quite big. It is fused to the N-terminus of a protein. However, many commercially-available sources of GST-tagged plasmids include a thrombin domain for cleavage of the GST tag during protein purification.
A GST-tag is often used to separate and purify as well as confirm expression of proteins that contain the GST-fusion. GST-fusion proteins can be produced in Escherichia coli, as recombinant proteins. The GST part binds its substrate, glutathione. Agarose beads can be coated with glutathione, and such glutathione-Agarose beads bind GST-proteins. These beads are then washed, to remove contaminating bacterial proteins. Adding free glutathione to beads that bind purified GST-proteins will release the GST-protein in solution. GST-tag antibody is a useful tool for confirming protein expression, localization of expressed proteins in cells, as well as affinity-binding of GST-tagged proteins.2
REFERENCES
1. Scheich, C. et al: BMC Biotechnol. 3:12, 2003
2. Nojima, H. et al: J. Biol. Chem. 278:15461-4, 2003
2. Nojima, H. et al: J. Biol. Chem. 278:15461-4, 2003
Products are for research use only. They are not intended for human, animal, or diagnostic applications.
(Click to Enlarge) Western Blot detection of GST-tag in bacterial lysates containing various GST-tagged proteins (MW: 52, 41, 54, and 78 kDa respectively) using GST antibody.
Details
Cat.No.: | CP10120 |
Antigen: | Purified recombinant GST-tag expressed in E. coli. |
Isotype: | Mouse IgG |
Species & predicted species cross- reactivity ( ): | GST-Tag |
Applications & Suggested starting dilutions:* | WB 1:1000 IP n/d IHC n/d ICC n/d FACS n/d |
Predicted Molecular Weight of protein: | 26 kDa (GST-tag) |
Specificity/Sensitivity: | Detects endogenous proteins without cross-reactivity with other family members. |
Storage: | Store at -20°C, 4°C for frequent use. Avoid repeated freeze-thaw cycles. |
*Optimal working dilutions must be determined by end user.
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Mouse Glutathione S-Transferase Antibody: Mouse Glutathione S-Transferase Antibody | Size: 100 ul | CAT.#: CP10120 | Price: $302.00 |