Product Sheet CB18672
Description
BACKGROUND Lysine-specific demethylase 1 (LSD1; also known as BHC110) is a flavin-dependent monoamine oxidase, which can demethylate mono- and di-methylated lysines, specifically histone 3, lysines 4 and 9 (H3K4 and H3K9).1 Through a FAD-dependent oxidative reaction, LSD1 speficially removes histone H3K4me2 to H3K4me1 or H3K4me0. When forming a complex with androgen receptor (and possibly other nuclear hormone receptors), LSD1 changes its substrates to H3K9me2. LSD1 relieves repressive histone marks by demethylation of histone H3 at lysine 9 (H3-K9), thereby leading to de-repression of androgen receptor target genes.2 Functions of other components in LSD1 complex, such as CoREST and BHC80, as well as p53, have been further studied.3, 4 It\'s now known LSD1 complex mediates a coordinated histone modification switch through enzymatic activities as well as histone modification readers in the complex.
REFERENCES
1. Forneris, F. et al: Trends Biochem Sci.33:181, 2008.
2. Culhane, J.C. & Cole, P. A.: Curr Opin Chem Biol. 11:561, 2007.
3. Urbanucci, A. et al: BMC Cancer. 8:219, 2008.
4. Tsai, W.W. et al: Mol. Cell. Biol. 28:5139, 2008.
2. Culhane, J.C. & Cole, P. A.: Curr Opin Chem Biol. 11:561, 2007.
3. Urbanucci, A. et al: BMC Cancer. 8:219, 2008.
4. Tsai, W.W. et al: Mol. Cell. Biol. 28:5139, 2008.
Products are for research use only. They are not intended for human, animal, or diagnostic applications.
(Click to Enlarge) Specific detection of LSD1 proteins by Western Blot, using LSD1 (2H5) Mouse Monoclonal Antibody, in various cell lysates: HeLa, NIH-3T3, and C6.
Details
Cat.No.: | CB18672 |
Antigen: | E. coli-expressed recombinant human LSD1 protein fragments. |
Isotype: | Mouse monoclonal IgG1 |
Species & predicted species cross- reactivity ( ): | Human, Mouse, Rat |
Applications & Suggested starting dilutions: | WB 1:1000 IP n/d IHC (Paraffin) n/d ICC n/d FACS n/d |
Predicted Molecular Weight of protein: | 110 kDa |
Specificity/Sensitivity: | Specifically detects endogenous levels of LSD1 proteins. Does not cross-react with other related proteins. |
Storage: | Store at -20°C, 4°C for frequent use. Avoid repeated freeze-thaw cycles. |
*Optimal working dilutions must be determined by end user.
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Monoclonal Lysine-Specific Demethylase 1 Antibody (2H5) Antibody: Monoclonal Lysine-Specific Demethylase 1 Antibody (2H5) | Size: 100 ul | CAT.#: CB18672 | Price: $302.00 |