Product Sheet CC1039
Description
BACKGROUND Histone H3 is one of the four histones, along with H2A, H2B and H4, which form the eukaryotic nucleosome octamer core; the nucleosome octamer winds ~146 DNA base-pairs. It is a highly conserved protein of 135 amino acid residues. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl groups to arginine, methyl or acetyl groups to lysine, and phosphorylation of serine or threonine residues. Methylation of lysine 9 has been associated with gene silencing.1 Acetylation of histone H3 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs). Acetylation of lysine14 is commonly seen in genes that are being actively transcribed into RNA.2 Phosphorylation of histone H3 at serine 10 occurs during mitosis and meiosis in a wide range of eukaryotes and has been shown to be required for proper chromosome transmission.3 In addition, it was found that the coactivator CARM1 methylates histone H3 at Arg17 and Arg26 and cooperates synergistically with p160-type coactivators (e.g., GRIP1, SRC-1, ACTR) and coactivators with histone acetyltransferase activity (e.g., p300, CBP) to enhance gene activation by steroid and nuclear hormone receptors (NR).4
REFERENCES
1. Schneider, R et al: Nature Cell Biol. 6:73, 2004.
2. Liang, G. et al.: Proc. Natl. Acad. Sci. USA 101:7357, 2004.
3. Hsu, J. Y. et al.: Cell 102:279, 2000.
4. Ma, H. et al: Curr. Biol. 11: 1981-1985, 2001.
2. Liang, G. et al.: Proc. Natl. Acad. Sci. USA 101:7357, 2004.
3. Hsu, J. Y. et al.: Cell 102:279, 2000.
4. Ma, H. et al: Curr. Biol. 11: 1981-1985, 2001.
Products are for research use only. They are not intended for human, animal, or diagnostic applications.
(Click to Enlarge) Histone H3 proteins (A) treated or untreated with CARM1 methyltransferase, HeLa (B), NIH/3T3 (C), and C6 (D) lysates were subjected to Western Blot analysis using Methyl-Histone H3 (Arg17) Antibody.
Details
Cat.No.: | CC1039 |
Antigen: | Arg17 and surrounding sequence of human Histone H3. |
Isotype: | Affinity Purified Rabbit IgG |
Species & predicted species cross- reactivity ( ): | Human, Mouse, Rat |
Applications & Suggested starting dilutions: | WB 1:1000 IP n/d IHC (Paraffin) n/d ICC n/d FACS n/d |
Predicted Molecular Weight of protein: | 19 kDa |
Specificity/Sensitivity: | Detects endogenous Methyl-human Histone H3 proteins. |
Storage: | Store at 4° C for frequent use; at -20° C for at least one year. |
*Optimal working dilutions must be determined by end user.
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Polyclonal Histone H3, Methyl-Arg17 Antibody: Polyclonal Histone H3, Methyl-Arg17 Antibody | Size: 100 ul | CAT.#: CC1039 | Price: $302.00 |