Product Sheet CP10388
Description
BACKGROUND The MSX family genes are one of the most highly conserved homeobox-containing genes that are identified in several animal species. The three mammalian members, MSX1, MSX2 and MSX3, share 98% homology in their protein domain. They play important roles in inductive tissue interactions during vertebrate organogenesis, and their differential expression mediates patterning, morphogenesis and tissue formation. They encode closely related homeoproteins that function as transcriptional repressors through interacting with the components of the core transcription complex as well as other homeoproteins, and promote apoptosis in developing limbs and cephalic neural crest. Although it is expressed in diverse tissues, MSX1 gene expression is mainly associated with multi-potent progenitor cell. It is strongly expressed in a region of highly proliferative, multi-potent cells that give rise to chondrogenic and osteogenic derivatives of the limb but absent in regions where cells stopped to proliferate and begun to undergo differentiation. MSX1 is also expressed at high levels in adult mouse uterine epithelium and decreases during pregnancy following embryonic implantation to regulate various aspects of uterine epithelial morphology and maintain the adult uterus in a morphogenetically and developmentally responsive state. It is detected at lower level in endometrium and placentomes particularly after 60 days of gestation in cows. Msx1 gene expression can be regulated by a bidirectional transcription generating long noncoding antisense (AS) RNA the expression which is linked to the Msx1 sense (S) RNA level.1 Moreover, it was shown that MSX1 plays important role in the development of preimplantation bovine embryos especially in regulating the expression of developmentally important genes by suppressing its transcript during bovine oocyte maturation and cleavage stage embryo development. In addition, it was shown that MSX1 is a major upstream regulator of the dopaminergic neuronal subtype specification. The MSX1 was found to be a downstream target of PHOX2B and activates the Delta–Notch pathway in neuroblastoma.2 MSX1 can coordinate with other transcriptional factors to regulate different pathways. It was shown that MSX1 and Pax9 interact synergistically throughout lower incisor development and affect multiple signaling pathways that influence incisor size and symmetry.3 Furthermore, Osr2was shown to actdownstream of Pax9 and patterns the mesenchymal odontogenic field through protein–protein interactions with Msx1 and Pax9 during early tooth development.4
REFERENCES
1. Babajko, S. et al: Cells Tissues Organs (DOI: 10.1159/000324466), 2011
2. Revet, I. et al: Exp. Cell Res. 314:707-19, 2008
3. Nakatomi, M. et al: Develop. Biol. 340:438-49, 2010
4. Zhou, J. et al: Develop. Biol. 353:344-53, 2011
2. Revet, I. et al: Exp. Cell Res. 314:707-19, 2008
3. Nakatomi, M. et al: Develop. Biol. 340:438-49, 2010
4. Zhou, J. et al: Develop. Biol. 353:344-53, 2011
Products are for research use only. They are not intended for human, animal, or diagnostic applications.
(Click to Enlarge) Western blot detection of MSX1 proteins in NTERA-2 cell lysate using MSX1 Antibody.
Details
Cat.No.: | CP10388 |
Antigen: | Raised against recombinant human MSX1 fragments expressed in E. coli. |
Isotype: | Mouse IgG1 |
Species & predicted species cross- reactivity ( ): | Human |
Applications & Suggested starting dilutions:* | WB 1:1000 IP n/d IHC n/d ICC n/d FACS n/d |
Predicted Molecular Weight of protein: | 31 kDa |
Specificity/Sensitivity: | Detects MSX1 proteins in various cell lysate. |
Storage: | Store at -20°C, 4°C for frequent use. Avoid repeated freeze-thaw cycles. |
*Optimal working dilutions must be determined by end user.
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Mouse MSX1 Antibody: Mouse MSX1 Antibody | Size: 100 ul | CAT.#: CP10388 | Price: $302.00 |