Product Sheet CC1035
Description
BACKGROUND Nuclear hormone receptors are ligand-dependent transcription factors that require coactivators to regulate target gene expression. The steroid receptor coactivator-3 (SRC-3), also known as p/CIP, RAC3, AIB1, ACTR and TRAM-1, is a cancer-amplified coactivator in the SRC gene family which also includes SRC-1 and SRC-2. SRC-3 interacts with nuclear receptors and a subset of transcription factors, recruits histone acetyltransferases and methyltransferases for chromatin remodeling, and facilitates target gene transcription.1 SRC-3 phosphorylation and methylation have been shown to regulate coactivator complex assembly.2,3 Accumulated results from both ex vivo and animal model studies indicate that SRC-3 plays important roles in many biological processes involving cell proliferation, cell migration, cell differentiation, somatic growth, sexual maturation, female reproductive function, and vasoprotection.1 Moreover, SRC-3 has been associated with multiple cancers, including breast, gastric and prostate cancers.4 The phosphorylation of Thr24 in SRC-3 can be mediated by activation of MAP kinase pathway, which may regulate function and localization of SRC-3 proteins in cells.5
REFERENCES
1. Liao, L. et al.: J Steriod Biochem Mol Biol 83:3, 2002.
2. Feng, Q. et al.: Mol Cell Biol 26:7846, 2006.
3. Wu, H. et al.: J Biol Chem 281:21848, 2006.
4. Yan, J. et al.: Acta Pharmacol Sin 27:387, 2006.
5. Wu, R.C. et al.: Mol. Cell 5:937-49, 2004.
2. Feng, Q. et al.: Mol Cell Biol 26:7846, 2006.
3. Wu, H. et al.: J Biol Chem 281:21848, 2006.
4. Yan, J. et al.: Acta Pharmacol Sin 27:387, 2006.
5. Wu, R.C. et al.: Mol. Cell 5:937-49, 2004.
Products are for research use only. They are not intended for human, animal, or diagnostic applications.
(Click to Enlarge) Neuregulin-stimulated MCF-7 cell lysates were treated with lambda phosphatase and subjected to Western Blot analysis using Anti-Phospho-SRC-3 (Thr24) Rabbit Polyclonal Antibody (Cat.# CC1035) (Top), or Anti-SRC-3 Monoclonal Antibody (Cat# CB10247) (Bottom).
Details
Cat.No.: | CC1035 |
Antigen: | Sequence surrounding and including Thr24 of human SRC-3/AIB-1. |
Isotype: | Affinity Purified Rabbit IgG |
Species & predicted species cross- reactivity ( ): | Human, Mouse |
Applications & Suggested starting dilutions: | WB 1:1000 IP n/d IHC (Paraffin) n/d ICC n/d FACS n/d |
Predicted Molecular Weight of protein: | 160 kDa |
Specificity/Sensitivity: | Detects endogenous phospho-human and mouse SRC-3/AIB-1. |
Storage: | Store at 4° C for frequent use; at -20° C for at least one year. |
*Optimal working dilutions must be determined by end user.
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Polyclonal SRC-3/AIB-1, Phospho-Thr24 Antibody: Polyclonal SRC-3/AIB-1, Phospho-Thr24 Antibody | Size: 100 ul | CAT.#: CC1035 | Price: $333.00 |