Product Sheet CC1028
Description
BACKGROUND VEGFR1 (Flt-1) and VEGFR2 (KDR/Flk-1) play an important role in regulating physiological as well as pathological angiogenesis. VEGFR2 has strong tyrosine kinase activity, and transduces the major signals for angiogenesis. However, unlike other representative tyrosine kinase receptors which use the Ras pathway, VEGFR2 mostly uses the Phospholipase-C-gamma-Protein kinase-C pathway to activate MAP-kinase and DNA synthesis. VEGFR2 is a direct signal transducer for pathological angiogenesis including cancer and diabetic retinopathy, thus, VEGFR2 itself and the signaling pathway appear to be critical targets for the suppression of these diseases.1,2 Upon binding to its ligand most commonly including VEGF-A and VEGF-E, VEGFR2 undergoes dimerization and becomes activated. Autophosphorylation of tyrosine 1175 is required for binding of PLC-gamma3 and Shb4 (Src homology 2 domain containing adaptor protein B).
REFERENCES
1. Olsson, A. K. et al: Nat Rev Mol Cell Biol 7:359-71, 2006.
2. Shibuya, M.: J Biochem Mol Biol. 39:469-78, 2006.
3. Takahashi, T. et al.: EMBO J. 20:2768-78, 2001.
4. Holmqvist, K et al.: J. Biol. Chem. 279:22267-75, 2004.
2. Shibuya, M.: J Biochem Mol Biol. 39:469-78, 2006.
3. Takahashi, T. et al.: EMBO J. 20:2768-78, 2001.
4. Holmqvist, K et al.: J. Biol. Chem. 279:22267-75, 2004.
Products are for research use only. They are not intended for human, animal, or diagnostic applications.
(Click to Enlarge) HUVEC (Cat# 200-05n) were starved overnight and then stimulated with VEGF-A (100ng/ml for 2 min). The cell lysates were subjected to Western Blot analysis using Phospho-VEGFR2 (Tyr1175) specific antibody (Cat# CC1028) (Top), and VEGFR2 Antibody (Cat# CB1020) (Bottom).
Details
Cat.No.: | CC1028 |
Antigen: | 15 amino acid residues surrounding and including human VEGFR2 phospho-Tyr1175. |
Isotype: | Affinity Purified Rabbit IgG |
Species & predicted species cross- reactivity ( ): | Human, Mouse |
Applications & Suggested starting dilutions: | WB 1:1000 IP n/d IHC (Paraffin) n/d ICC n/d FACS n/d |
Predicted Molecular Weight of protein: | 230 kDa |
Specificity/Sensitivity: | This antibody detects endogenous levels of phosphorylated VEGFR2. |
Storage: | Store at 4° C for frequent use; at -20° C for at least one year. |
*Optimal working dilutions must be determined by end user.
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Polyclonal VEGF Receptor 2, Phospho-T1175 Antibody: Polyclonal VEGF Receptor 2, Phospho-T1175 Antibody | Size: 100 ul | CAT.#: CC1028 | Price: $302.00 |
Resources/Documents
Publications
2017
Bailey, E., Y Cui, A. Casey, J. Stoler, X. AI, D. Ma, R. Handin, P. Sliz, S. Vargas and S. El-Chemaly. Pulmonary Vasculopathy Associated with FIGF Gene Mutation. Am J Pathol, 187:25-32.
2016
Bailey, E., Y. Cui, A. Casey, J. Stoler, X. Ai, D. Ma, R. Handin, P. Sliz, S. Vargas and S. El-Chemaly. 2016. Pulmonary Vasculopathy Associated with FIGF Gene Mutation. Am J Pathol, 187:25-32.
2011
Tammali, R., A.M. Reddy, S. Srivastava, and K. Ramana. 2011. Inhibition of aldose reductase prevents angiogenesis in vitro and in vivo. Angiogenesis. 14:209-221.