Product Sheet CB14574

Description

BACKGROUND Transcription Factor II B (TF IIB) is an essential component of the RNA polymerase II (Pol II) machinery that acts as a bridge between the TATA-binding protein (TBP) and Pol II.1 The C-terminal domain of TFIIB interacts with the TBP-TATA complex, whereas the N-terminal domain is required for the assembly of TFIIF and Pol II into the preinitiation complex. It is involved in the selection of the transcription start site: mutations in TFIIB cause a shift in the transcription start site.2 In addition, TFIIB interacts with certain TBP-associated factors (TAFs) in vitro, and these interactions might contribute to recruitment or stable association of TFIIB in the context of an active transcription complex.3
 
REFERENCES 
1. Ha, I. et al: Nature 352:689, 1991.
2. Lee, T. I. & Young,  R. A. : Annu. Rev. Genet. 34:77, 2000.
3. Goodrich, J. A. et al: Cell 75:519, 1993.
 
Products are for research use only. They are not intended for human, animal, or diagnostic applications.

(Click to Enlarge) Specific detection of TFIIB proteins by Western Blot, using TFIIB (48C6) Mouse Monoclonal Antibody, in various cell lysates: HeLa, HEK293, and MCF7.

Details

Cat.No.:
CB14574
Antigen:
E. coli-expressed recombinant human TFIIB protein fragments.
Isotype:
Mouse monoclonal IgG2b
Species & predicted
species cross-
reactivity ( ):
Human
Applications &
Suggested starting
dilutions:
WB                  1:1000
IP                    n/d
IHC (Paraffin)  n/d
ICC                 n/d
FACS              n/d
Predicted Molecular
Weight of protein:
38 kDa
Specificity/Sensitivity:
Specifically detects endogenous levels of human TFIIB proteins. Does not cross-react with other related proteins.
Storage:
Store at -20°C, 4°C for frequent use. Avoid repeated freeze-thaw cycles.

 *Optimal working dilutions must be determined by end user.

Products

Product Size CAT.# Price Quantity
Monoclonal Transcription Factor II B Antibody (48C6) Antibody: Monoclonal Transcription Factor II B Antibody (48C6) Size: 100 ul CAT.#: CB14574 Price: $302.00
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