MSDS Cryopreserved Cells
Instructions BAOEC
5 Important Cell Culture Rules
Cell Apps Flyer Cardiovascular Cells
Cell Apps Flyer Endothelial Cells
Cell Apps Poster Primary Cells
Cell Applications Inc Brochure

Description

Bovine aortic endothelial cells (BAOEC) provide an excellent model system to study all aspects of cardiovascular function and disease, such as critical signaling pathways and mechanisms relevant to proper function of endothelia, including angiogenesis, permeability, flow adaptation, NO production, and diabetes-related complications.  BAOEC can also be used to search for beneficial modulators and delivery systems for therapeutic use, and to design scaffolds, surfaces and materials for tissue engineering and 3D modeling.
BAOEC from Cell Applications, Inc. have been used in dozens of research publications, for example to:
  • Determine that simvastatin modulates beta-adrenergic signaling in vascular wall by inhibiting cAMP accumulation in response to epinephrine in a mechanism involving downregulation of Galpha(s) translation via Akt/mTOR/eIF4/4EBP pathway
  • Elucidate the PKA-a signaling in endothelial cells by showing that it directly phosphorylates FoxO1 to regulate expression of VCAM-1
  • Identify CaMKKβ and LKB1 as critical determinants of simvastatin-dependent activation of AMPK- and Rac1-modulated signaling and reveal that Rac1 in turn regulates LKB1 phosphorylation
  • Demonstrate that Chondromodulin-I (ChM-I), a cartilage-derived angiogenesis inhibitor, impairs the VEGF-A-induced  Rac1/Cdc42 activity leading to destabilized lamellipodial extensions and decreased motility of endothelial cells
  • Demonstrate that cofilin-mediated actin alignment in the direction of shear stress is required for endothelial barrier integrity and that p190RhoGAP links integrins and caveolin-1/caveolae to RhoA in a mechanotransduction cascade that participates in endothelial adaptation to flow
  • Show that activation of RhoA/ROCK/p38 MAPK pathway causes increased endothelial arginase activity/expression and is the key mediator of endothelial dysfunction and decreased NO production precipitated by either oxidative stress-activated PKC, or by angiotensin II-activated Gα12/13 G proteins coupled to AT1 receptors
  • Demonstrate that activation of mTOR/p70S6K by angiotensin II may contribute to impairment of insulin-stimulated vasodilation through phosphorylation of IRS-1 at Ser636/639
  • Discover that testosterone induces a non-genomic membrane-initiated Ca2+ dependent signaling pathway that leads to activation of NF-kB, providing an explanation for why men are predisposed to earlier onset of atherosclerosis
  • Demonstrate that increase in salt concentration, observed in hypertension patients or in individuals with high salt intake, suppresses NO synthase activity, contributing to the development of hypertension
  • Show that valsartan, a selective angiotensin II type 1 receptor (AT1R) blocker, increases NO production via Src/PI3K/Akt signaling that leads to phosphorylation of NO synthase
  • Determine that long-chain polyunsaturated fatty acids exert their beneficial effects on cardiovascular health via activation of B2 receptor leading to elevated expression of NO synthase
  • Show that transient receptor potential vanilloid type 1 (TRPV1) activation by evodiamine or capsaicin initiates two Ca2+-dependent signaling pathways, both resulting in activation of NO synthase: one is PI3K/Akt/CaMKII activation, which leads to NO synthase phosphorylation at Ser1179 and Ser635, and the other involves PP2B-dependent dephosphorylation of PKC leading to decreased phosphorylation of NO synthase at Thr497
  • Discover that LDL-induced endothelial disfunction is mediated by epigenetic upregulation of p66shc promoter increasing expression of p66shc which then stimulates expression of ICAM-1 and inhibits expression of thrombomodulin, leading to stimulated adhesion of monocytes and to plasma coagulation on the surface of endothelial cells
  • Elucidate the involvement of AMPK cascade and autophagy in mediating beneficial cardiovascular effects of green tea;
  • Show, along with Bovine Brain Microvascular Endothelial Cells, also from Cell Applications, Inc., that brain microvasculature is more sensitive to pathogenesis, compared to large vessel endothelia, by demonstrating that C-reactive protein (CRP), a cardiovascular risk factor, induces higher oxidative stress in the brain microvasculature due to higher local expression of the CRP-receptors CD16, CD32 and of the NAD(P)H-oxidase subunit p22phox, and that brain microvascular endothelial cells show higher sensitivity to oxidative stress generated by advanced glycation end products due to stronger VEGF expression leading to increased permeability
  • Improve the efficiency of small molecule cancer therapeutics by prolonging their cytoplasm stay
  • Construct an expression cassette to maximize targeted transgene expression in large vessel endothelia, develop new viral-based vectors an evaluate apoA-I, IL-10 and NO synthase potential for for atheroprotective human gene therapy, and design scaffolds, surfaces and materials for tissue engineering and 3D modeling, as well as for therapies aimed to prevent stent thrombosis
  • Show that erythropoietin (EPO)-mediated activation of NO synthase involves AMPK-dependent signaling, which leads to enhanced phosphorylation of βCR and NO synthase
(Click to Enlarge) Bovine Aortic Endothelial Cell (BAOEC) monolayer (L) & BAOEC labeled w/ DiI-Ac-LDL, the acetylated apoprotein specifically recognized & endocytosed by endothelial cells (R).  

Details

Tissue
Healthy, normal aorta of USDA-inspected cattle
QC
No bacteria, yeast, fungi, mycoplasma
Character
DiI-Ac-LDL uptake: Positive
Bioassay
Attach, spread, proliferate in Growth Med
Cryopreserved
500,000 BAOEC (2nd passage) frozen in Basal Medium w/ 10% FBS, 10% DMSO
Kit
Cryovial frozen BAOEC (B304-05), Growth Medium (B211-500), Subculture Rgnt Kit (090K)
Proliferating
Shipped in Gr Med, 3rd psg (flasks or plates)
Doublings
At least 16
Applications
Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
Instructions BAOEC

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MSDS Cryopreserved Cells

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Products

Product Size CAT.# Price Quantity
Cryopreserved BAOEC: Frozen BAOEC Size: 1 Cryovial CAT.#: B304-05 Price: $586.56
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Cryopreserved BAOEC Total Kit: 5x10^5 Cells, Medium & Subculture Reagents (See Details tab for specifics) Size: 1 Kit CAT.#: B304K-05 Price: $735.59
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Proliferating BAOEC: Actively growing, dividing cells in medium, pooled Size: T-25 Flask CAT.#: B305-25 Price: $586.56
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Proliferating BAOEC: Actively growing, dividing cells in medium, pooled Size: T-75 Flask CAT.#: B305-75 Price: $791.04
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Proliferating BAOEC: Actively growing, dividing cells in medium, pooled Size: 6 Well CAT.#: B305-6W Price: $737.23
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Proliferating BAOEC: Actively growing, dividing cells in medium, pooled Size: 96 Well CAT.#: B305-96W Price: $866.38
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Related Products

Product Size CAT.# Price Quantity
Bovine Endothelial Cell Basal Medium: Basal medium (contains no growth supplement).  Add GS before use. Size: 500 ml CAT.#: B210-500 Price: $57.04
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Bovine Endothelial Cell Growth Medium: All-in-one ready-to-use Size: 500 ml CAT.#: B211-500 Price: $105.47
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Bovine Endothelial Cell Growth Medium Kit: Basal medium & growth supplement sold together packaged separately Size: Yields 500 ml CAT.#: B211K-500 Price: $113.01
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Bovine Endothelial Cell Growth Supplement: Added to Basal Medium to create Growth Medium Size: 30 ml CAT.#: B211-GS Price: $50.58
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Extended Family Products

Product Size CAT.# Price Quantity
BAOEC RNA: Total RNA prepared from Bovine Aortic Endothelial Cells Size: 10 ug CAT.#: B304-R10 Price: $349.78
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BAOEC RNA: Total RNA prepared from Bovine Aortic Endothelial Cells Size: 25 ug CAT.#: B304-R25 Price: $699.56
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Cell Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO. Size: 50 ml CAT.#: 040-50 Price: $53.81
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Cyto-X Colorimetric Cell Counting Reagent: 500 tests Size: 1 Bottle CAT.#: 028-01 Price: $138.84
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Cyto-X Colorimetric Cell Counting Reagent: 100 tests Size: Sample CAT.#: 028-S Price: $35.88
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Cytofect™ Endothelial Cell Transfection Kit: 25 x 24-Well Rxns Size: 1 Sample Kit CAT.#: TF101KS Price: $53.76
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Cytofect™ Endothelial Cell Transfection Kit: 250 x 24-Well Rxns Size: 1 Kit CAT.#: TF101K Price: $430.50
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Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution Size: 1 Kit CAT.#: 090K Price: $54.89
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Resources/Documents

5 Important Cell Culture Rules

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Cell Apps Flyer Cardiovascular Cells

Format: PDF

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Cell Apps Flyer Endothelial Cells

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Cell Apps Poster Primary Cells

Format: PDF

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Cell Applications Inc Brochure

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Publications

2017

Wang, S., Liang, S., Zhao, X., He, Y. and Qi, Y., 2017. Propofol inhibits cell proliferation and invasion in rheumatoid arthritis fibroblast-like synoviocytes via the nuclear factor-κB pathway. American journal of translational research, 9(5), p.2429.

Berger, A.J., Linsmeier, K., Kreeger, P.K. and Masters, K.S., 2017. Decoupling the effects of stiffness and fiber density on cellular behaviors via an interpenetrating network of gelatin-methacrylate and collagen. Biomaterials. https://doi.org/10.1016/j.biomaterials.

Dang, L.T., Aburatani, T., Marsh, G.A., Johnson, B.G., Alimperti, S., Yoon, C.J., Huang, A., Szak, S., Nakagawa, N., Gomez, I. and Ren, S., 2017. Hyperactive FOXO1 results in lack of tip stalk identity and deficient microvascular regeneration during kidney injury. Biomaterials. https://doi.org/10.1016/j.biomaterials.2017.07.010

Hara, T., E. Yoshida, Y. Shinkai, C. Yamamoto, Y. Fujiwara, Y. Kumagai and T. Kaji. 2017. Biglycan Intensifies ALK5–Smad2/3 Signaling by TGF-β1 and Downregulates Syndecan-4 in Cultured Vascular Endothelial Cells. J Cell Biochem, DOI: 10.1002/jcb.25721
He, D., Y. Ariska, K. Masuda, M. Yamamoto and N. Takeda. 2017. A Photoresponsive Soft Interface Reversibly Controls Wettability and Cell Adhesion by Conformational Changes in a Spiropyran-conjugated Amphiphilic Block Copolymer. Acta Biomateriala, dx.doi.org/10.1016/j.actbio.2017.01.049.
2016
Chen, X., Z. Cheng, D. Werling, G. Pollott, M. Salavati, K. Johnson, F. Khan, D. Wathes and S. Zhang. 2016. Bovine P-selectin mediates leukocyte adhesion and is highly polymorphic in dairy breeds. Research in Veterinary Science, 108:85-92.
DelloStritto, D., P. Connell, G. Dick, I. Fancher, B. Klarich, J. Fahmy, P. Kang, Y. Chen, D. Damron, C. Thodeti and I. Bratz. 2016. Differential regulation of TRPV1 channels by H2O2: implications for diabetic microvascular dysfunction. Basic Research in Cardiology, 111:21.
Dronadula, N., B. Wacker, R. Van Der Kwast, J. Zhang and D. Dichek. 2016. Stable in Vivo Transgene Expression in Endothelial Cells with Helper-Dependent Adenovirus: Roles of Promoter and Interleukin-10. Human Gene Therapy, doi:10.1089/hum.2016.134.
Farwell, S., D. Kanyi, M. Hamel, J. Slee, E. Miller, M. Cipolle, and L. Lowe-Krentz. 2016. Heparin decreases in TNFα;-induced endothelial stress responses require transmembrane protein 184A and induction of dual specificity phosphatase-1. J Biol Chem, 291:5342-5354.
Fujie, T., M. Murakami, E. Yoshida, T. Tachinami, Y. Shinkai, Y. Fujiwara, C. Yamamoto, Y. Kumagai, H. Naka and T. Kaji. 2016. Copper diethyldithiocarbamate as an activator of Nrf2 in cultured vascular endothelial cells. J Biolog Inorganic Chem, 21:263-273.
Fujie, T., Y. Segawa, A. Uehara, T. Nakamura, T. Kimura, E. Yoshida, C. Yamamoto, M. Uchiyama, H. Naka and T. Kaji. 2016. Zinc diethyldithiocarbamate as an inducer of metallothionein in cultured vascular endothelial cells. J Toxicolog Sci, 41:217-224.
Fujie, T., Y. Segawa, E. Yoshida, T. Kimura, Y. Fujiwara, C. Yamamoto, M. Satoh, H. Naka and T. Kaji. 2016. Induction of metallothionein isoforms by copper diethyldithiocarbamate in cultured vascular endothelial cells. J Toxicolog Sci, 41:225-232.
Hara, T., H. Matsuzaki, T. Nakamura, E. Yoshida, T. Ohkubo, H. Maruyama, C. Yamamoto, S. Saito and T. Kajo. 2016. Cytotoxicity of zinc, copper and Rhodium complexes with 1,10-phenanthroline or 2,9-dimethyl-1,10-phenanthroline in cultured vascular endothelial cells. Fundam Toxicol Sci, 3:109-113.
Hara, T., E. Yoshida, Y. Fujiwara, C. Yamamoto and T. Kaji. 2016. Transforming growth factor-β1 modulates the expression of syndecan-4 in cultured vascular endothelial cells in a biphasic manner. J Cell Biochem, DOI 10.1002/jcb.25861.
Hara, T., E. Yoshida, Y. Shinkai, C. Yamamoto, Y. Fujiwara, Y. Kumagai, and T. Kaji. 2016. Biglycan Intensifies ALK5-Smad2/3 Signaling by TGF-β1 and Downregulates Syndecan-4 in Cultured Vascular Endothelial Cells. J Cellular Biochem, DOI: 10.1002/jcb.25721.
Pugh, R., J. Slee, S. Farwell, Y. Li, T. Barthol, W. Patton and L. Lowe-Krentz. 2016. Transmembrane protein 184A is a receptor required for vascular smooth muscle cell responses to heparin. JBC, 291:5326-5341.
Yu, Y., K. Su, Y. Kou, B. Guo, K. Lee, J. Wei and T. Lee. 2016. Role of transient receptor potential vanilloid 1 in regulating erythropoietin-induced activation of endothelial nitric oxide synthase. Acta Physiologica, DOI: 10.1111/apha.12723.
2014
Bloom, A., and N. Winograd. 2014. Dye‐enhanced imaging of mammalian cells with SIMS. Surface and Interface Analysis. 46:177-180.
Browning, M., V. Guiza, B. Russel, J. Rivera, S. Cereceres, M. Höök, M. Hahn, and E. Cosgriff-Hernandez. 2014. Endothelial cell response to chemical, biological, and physical cues in bioactive hydrogels. Tissue Engineering Part A, 20:3130-3141.
Hu, J., C. Hardy, C. Chen, S. Yang, A. Voloshin and Y. Liu. 2014. Enhanced Cell Adhesion and Alignment on Micro-Wavy Patterned Surfaces. PLoS One, dx.doi.org/10.1371/journal.pone.0104502. BAOEC
Gardner, C, J. Trisnadi, T. Kim, K. Brammer, L. Reiss, L. Chen and S. Jin. 2014. Controlled metallic nanopillars for low impedance biomedical electrode. Acta Biomaterialia, 10:2296-2303.
Juffermans, L., B. Meijering, R. Henning and L. Deelman. 2014. Ultrasound and Microbubble-Targeted Delivery of Small Interfering RNA Into Primary Endothelial Cells Is More Effective Than Delivery of Plasmid DNA. Ultrasound in Med & Biol, 40:532-540.
Miyashita, S., K. Niwa, T. Watanabe, and Y. Sagane. 2014. Host-cell specificity and transcytosis of nontoxic nonhemagglutinin protein of botulinum neurotoxin serotype D. FEMS Microbiology Letters, Online: 1 August.
Pun, P., A. Logan, V. Darley-Usmar, B. Chacko, M. Johnson, G. Huang, S. Rogatti, T. Prime, C. Methner, T. Krieg, I. Fearnley, L. Larsen, D. Larsen, K. Menger, Y. Collins, A. James, G. Kumar, R. Hartley, R. Smith and M. Murphy. 2014. A mitochondria-targeted mass spectrometry probe to detect glyoxals: implications for diabetes. Free Rad Biol & Med, 67:437-450.
Rahman, W., S. Corde, N. Yagi, S. Aziz, N. Annabell, and M. Geso. 2014. Optimal energy for cell radiosensitivity enhancement by gold nanoparticles using synchrotron-based monoenergetic photon beams. International Journal of Nanomedicine, 9:2459–2467.
Shatanawi, A., M. Gharaibeh, R. Caldwell and R. Caldwell. 2014. High Glucose Upregulates Arginase 1 and Decreases Nitric Oxide Production through ATF-2 and c-Jun Transcription Factors. Life Sci J, 11:374-379.
Weibull, E., S. Matsui, H. Svahn and T. Ohashi. 2014. A microfluidic device towards shear stress analysis of clonal expanded endothelial cells. J Biomech Sci & Eng, 9: p. JBSE006.
2014
Bloom, A., and N. Winograd. 2014. Dye‐enhanced imaging of mammalian cells with SIMS. Surface and Interface Analysis. 46:177-180.
Browning, M., V. Guiza, B. Russel, J. Rivera, S. Cereceres, M. Höök, M. Hahn, and E. Cosgriff-Hernandez. 2014. Endothelial cell response to chemical, biological, and physical cues in bioactive hydrogels. Tissue Engineering Part A, 20:3130-3141.
Miyashita, S., K. Niwa, T. Watanabe, and Y. Sagane. 2014. Host-cell specificity and transcytosis of nontoxic nonhemagglutinin protein of botulinum neurotoxin serotype D. FEMS Microbiology Letters, Online: 1 August.
Pun, P.B., A. Logan, V. Darley-Usmar, B. Chacko, M.S. Johnson, G.W. Huang, S. Rogatti, T.A. Prime, C. Methner, T. Krieg, I.M. Fearnley, L. Larsen, D. Larsen, K.E. Menger, Y. Collins, A.M. James, G.D. Kishore Kumar, R.C. Hartley, R.A.J. Smith, and M.P. Murphy. 2014. A mitochondria-targeted mass spectrometry probe to detect glyoxals: Implications for diabetes. Free Radical Biology and Medicine. 67:437-50
Rahman, W., S. Corde, N. Yagi, S. Aziz, N. Annabell, and M. Geso. 2014. Optimal energy for cell radiosensitivity enhancement by gold nanoparticles using synchrotron-based monoenergetic photon beams. International Journal of Nanomedicine, 9:2459–2467.
2013
Candelario, J., and M. Chachisvilis. 2013. Activity of Bradykinin B2 Receptor Is Regulated by Long-Chain Polyunsaturated Fatty Acids. PloS one. 8:e68151.
Ching, L.C., J.F. Zhao, K.H. Su, S.K. Shyue, C.P. Hsu, T.M. Lu, S.J. Lin, and T.S. Lee. 2013. Activation of transient receptor potential vanilloid 1 decreases endothelial nitric oxide synthase phosphorylation at Thr497 by protein phosphatase 2B-dependent dephosphorylation of protein kinase C. Acta Physiologica. 209:124-135.
Huang, J.-H., J. Kim, Y. Ding, A. Jayaraman, and V.M. Ugaz. 2013. Embedding Synthetic Microvascular Networks in Poly(Lactic Acid) Substrates with Rounded Cross-Sections for Cell Culture Applications. PloS one. 8:e73188.
Kim, H.-S., V. Montana, H.-J. Jang, V. Parpura, and J.-a. Kim. 2013. Epigallocatechin-gallate (EGCG) stimulates autophagy in vascular endothelial cells: A potential role for reducing lipid accumulation. Journal of Biological Chemistry. 288:22706. doi:22710.21074/jbc.P22113.477505
Slee, J.B., and L.J. Lowe-Krentz. 2013. Actin realignment and cofilin regulation are essential for barrier integrity during shear stress. Journal of cellular biochemistry. 114:782-795.
2012
Chandra, S., M. Romero, A. Shatanawi, A. Alkilany, R. Caldwell and R. Caldwell. 2012. Oxidative species increase arginase activity in endothelial cells through the RhoA/Rho kinase pathway. British J Pharmacol, 165:506-519.
Ching, L., C. Chen, K. Su, H. Hou, S. Shyue, Y. Kou and T. Lee. 2012. Implication of AMP-Activated Protein Kinase in Transient Receptor Potential Vanilloid Type 1–Mediated Activation of Endothelial Nitric Oxide Synthase. Molec Med, 18:805-815
Hogg, P. and P. Dilda. 2012. Organo-arsenoxide compounds and use thereof. Patent US 8268883 B2.
Hou, H.-H., B.D. Hammock, K.-H. Su, C. Morisseau, Y.R. Kou, S. Imaoka, A. Oguro, S.-K. Shyue, J.-F. Zhao, and T.-S. Lee. 2012. N-terminal domain of soluble epoxide hydrolase negatively regulates the VEGF-mediated activation of endothelial nitric oxide synthase. Cardiovascular Research. 93:120-129.
Jiang, B., L. Du, R. Flynn, N. Dronadula, J. Zhang, F. Kim, and D. Dichek. 2012. Overexpression of Endothelial Nitric Oxide Synthase Improves Endothelium-Dependent Vasodilation in Arteries Infused with Helper-Dependent Adenovirus. Human Gene Therapy. 23:1166-1175.
Kim, J.-a., H.-J. Jang, L.A. Martinez-Lemus, and J.R. Sowers. 2012. Activation of mTOR/p70S6 kinase by ANG II inhibits insulin-stimulated endothelial nitric oxide synthase and vasodilation. American Journal of Physiology - Endocrinology and Metabolism. 302:E201-E208.
Kim, Y.-R., C.-S. Kim, A. Naqvi, A. Kumar, S. Kumar, T.A. Hoffman, and K. Irani. 2012. Epigenetic upregulation of p66shc mediates low-density lipoprotein cholesterol-induced endothelial cell dysfunction. American Journal of Physiology - Heart and Circulatory Physiology. 303:H189-H196.
Kitagawa, T., H. Kosuge, M. Uchida, M. Dua, Y. Iida, R. Dalman, T. Douglas, and M. McConnell. 2012. RGD-Conjugated Human Ferritin Nanoparticles for Imaging Vascular Inflammation and Angiogenesis in Experimental Carotid and Aortic Disease. Mol Imaging Biol. 14:315-324.
Kou, R., T. Shiroto, J.L. Sartoretto, and T. Michel. 2012. Suppression of Gαs synthesis by simvastatin treatment of vascular endothelial cells. The Journal of biological chemistry. 287:2643-2651.
McGrath, K., X. Li, K. Gaus, P. Williams, D. Celermajer, D. Handelsman, and A. Heather. 2012. Androgens Rapidly Activate Nuclear Factor-Kappa B via Intracellular Ca 2+ Signalling in Human Vascular Endothelial Cells. Journal of Steroids & Hormonal Science:S2:005. doi:010.4172/2157-7536.S4172-4005.
Niiya, Y., T. Abumiya, S.-i. Yamagishi, J.-i. Takino, and M. Takeuchi. 2012. Advanced Glycation End Products Increase Permeability of Brain Microvascular Endothelial Cells through Reactive Oxygen Species–Induced Vascular Endothelial Growth Factor Expression. Journal of Stroke and Cerebrovascular Diseases. 21:293-298.
Su, K., Y. Yu, H. Hou, J. Zhao, Y. Kou, L. Cheng, S. Shyue and T. Lee. 2012. AMP-activated protein kinase mediates erythropoietin-induced activation of endothelial nitric oxide synthase. J Cell Physiol, 227:3053-3062.
2011
Baek, J.H., C.E.N. Reiter, D.J. Manalo, P.W. Buehler, R.C. Hider, and A.I. Alayash. 2011. Induction of hypoxia inducible factor (HIF-1α) in rat kidneys by iron chelation with the hydroxypyridinone, CP94. Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms. 1809:262-268.
Ching, L.-C., Y.R. Kou, S.-K. Shyue, K.-H. Su, J. Wei, L.-C. Cheng, Y.-B. Yu, C.-C. Pan, and T.-S. Lee. 2011. Molecular mechanisms of activation of endothelial nitric oxide synthase mediated by transient receptor potential vanilloid type 1. Cardiovascular Research. 91:492-501.
Dronadula, N., L. Du, R. Flynn, J. Buckler, J. Kho, Z. Jiang, S. Tanaka, and D. Dichek. 2011. Construction of a novel expression cassette for increasing transgene expression in vivo in endothelial cells of large blood vessels. Gene Therapy, 18:501-508.
Du, L., N. Dronadula, S. Tanaka, and D.A. Dichek. 2011. Helper-dependent adenoviral vector achieves prolonged, stable expression of interleukin-10 in rabbit carotid arteries but does not limit early atherogenesis. Hum Gene Ther. 22:959-968.
Fatisson, J., F. Azari, and N. Tufenkji. 2011. Real-time QCM-D monitoring of cellular responses to different cytomorphic agents. Biosensors and Bioelectronics. 26:3207-3212.
Flynn, R., K. Qian, C. Tang, N. Dronadula, J.M. Buckler, B. Jiang, S. Wen, H.L. Dichek, and D.A. Dichek. 2011. Expression of Apolipoprotein A-I in Rabbit Carotid Endothelium Protects Against Atherosclerosis. Molecular therapy : the journal of the American Society of Gene Therapy. 19:1833-1841.
Fossey, S., M. Bear, W. Kisseberth, M. Pennell, and C. London. 2011. Oncostatin M promotes STAT3 activation, VEGF production, and invasion in osteosarcoma cell lines. BMC cancer. 11:125.
Junkin, M. 2011. Microenvironments for study of myogenesis spatial organization and endothelial cell small messenger signaling. The University of Arizona, PhD dissertation.
Lee, J.-W., H. Chen, P. Pullikotil, and M.J. Quon. 2011. Protein Kinase A-α Directly Phosphorylates FoxO1 in Vascular Endothelial Cells to Regulate Expression of Vascular Cellular Adhesion Molecule-1 mRNA. Journal of Biological Chemistry. 286:6423-6432.
Shatanawi, A., M.J. Romero, J.A. Iddings, S. Chandra, N.S. Umapathy, A.D. Verin, R.B. Caldwell, and R.W. Caldwell. 2011. Angiotensin II-induced vascular endothelial dysfunction through RhoA/Rho kinase/p38 mitogen-activated protein kinase/arginase pathway. American Journal of Physiology - Cell Physiology. 300:C1181-C1192.
Slyvka, Y., Z. Wang, J. Yee, S.R. Inman, and F.V. Nowak. 2011. Antioxidant diet, gender and age affect renal expression of nitric oxide synthases in obese diabetic rats. Nitric Oxide. 24:50-60.
Su, K.-H., S.-K. Shyue, Y.R. Kou, L.-C. Ching, A.-N. Chiang, Y.-B. Yu, C.-Y. Chen, C.-C. Pan, and T.-S. Lee. 2011. β Common receptor integrates the erythropoietin signaling in activation of endothelial nitric oxide synthase. Journal of cellular physiology. 226:3330-3339.
Yang, B., C. Radel, D. Hughes, S. Kelemen, and V. Rizzo. 2011. p190 RhoGTPase-Activating Protein Links the β1 Integrin/Caveolin-1 Mechanosignaling Complex to RhoA and Actin Remodeling. Arteriosclerosis, Thrombosis, and Vascular Biology. 31:376-383.
2010
Closhen, D., B. Bender, H.J. Luhmann, and C.R.W. Kuhlmann. 2010. CRP-induced levels of oxidative stress are higher in brain than aortic endothelial cells. Cytokine. 50:117-120.
Flynn, R., J. Buckler, C. Tang, F. Kim and D. Dichek. 2010. Helper-dependent Adenoviral Vectors are Superior In Vitro to First-generation Vectors for Endothelial Cell-targeted Gene Therapy. Molec Ther, 18:2121-2129.
Loya, M.C., K.S. Brammer, C. Choi, L.-H. Chen, and S. Jin. 2010. Plasma-induced nanopillars on bare metal coronary stent surface for enhanced endothelialization. Acta Biomaterialia. 6:4589-4595.
Miura, S., K. Mitsui, T. Heishi, C. Shukunami, K. Sekiguchi, J. Kondo, Y. Sato, and Y. Hiraki. 2010. Impairment of VEGF-A-stimulated lamellipodial extensions and motility of vascular endothelial cells by chondromodulin-I, a cartilage-derived angiogenesis inhibitor. Experimental cell research. 316:775-788.
Reiter, C.E.N., J.-a. Kim, and M.J. Quon. 2010. Green Tea Polyphenol Epigallocatechin Gallate Reduces Endothelin-1 Expression and Secretion in Vascular Endothelial Cells: Roles for AMP-Activated Protein Kinase, Akt, and FOXO1. Endocrinology. 151:103-114.
Thebaud, N. 2010. Cellules endothéliales issues de progéniteurs humains : des acteurs pertinents en ingénierie vasculaire? PhD Dissertation, L’UNIVERSITÉ BORDEAUX 2.
2009
Dilda, P.J., S.p. Decollogne, L. Weerakoon, M.D. Norris, M. Haber, J.D. Allen, and P.J. Hogg. 2009. Optimization of the Antitumor Efficacy of a Synthetic Mitochondrial Toxin by Increasing the Residence Time in the Cytosol. Journal of Medicinal Chemistry. 52:6209-6216.
Kou, R., J. Sartoretto, and T. Michel. 2009. Regulation of Rac1 by Simvastatin in Endothelial Cells: DIFFERENTIAL ROLES OF AMP-ACTIVATED PROTEIN KINASE AND CALMODULIN-DEPENDENT KINASE KINASE-β. Journal of Biological Chemistry. 284:14734-14743.
Li, J., J. White, L. Guo, X. Zhao, J. Wang, E.J. Smart, and X.A. Li. 2009. Salt inactivates endothelial nitric oxide synthase in endothelial cells. The Journal of nutrition. 139:447-451.
Meijering, B.D.M., L.J.M. Juffermans, A. van Wamel, R.H. Henning, I.S. Zuhorn, M. Emmer, A.M.G. Versteilen, W.J. Paulus, W.H. van Gilst, K. Kooiman, N. de Jong, R.J.P. Musters, L.E. Deelman, and O. Kamp. 2009. Ultrasound and Microbubble-Targeted Delivery of Macromolecules Is Regulated by Induction of Endocytosis and Pore Formation. Circulation research. 104:679-687.
Su, K.-H., J.-Y. Tsai, Y.R. Kou, A.-N. Chiang, S.-H. Hsiao, Y.-L. Wu, H.-H. Hou, C.-C. Pan, S.-K. Shyue, and T.-S. Lee. 2009. Valsartan regulates the interaction of angiotensin II type 1 receptor and endothelial nitric oxide synthase via Src/PI3K/Akt signalling. Cardiovascular Research. 82:468-475.
2008
Chen, H., A.S. Lin, Y. Li, C.E.N. Reiter, M.R. Ver, and M.J. Quon. 2008. Dehydroepiandrosterone Stimulates Phosphorylation of FoxO1 in Vascular Endothelial Cells via Phosphatidylinositol 3-Kinase- and Protein Kinase A-dependent Signaling Pathways to Regulate ET-1 Synthesis and Secretion. Journal of Biological Chemistry. 283:29228-29238.
Chen, H., Y.C. Levine, D.E. Golan, T. Michel, and A.J. Lin. 2008. Atrial Natriuretic Peptide-initiated cGMP Pathways Regulate Vasodilator-stimulated Phosphoprotein Phosphorylation and Angiogenesis in Vascular Endothelium. Journal of Biological Chemistry. 283:4439-4447.
Fan, L.J., and T. Karino. 2008. Effect of serum concentration on adhesion of monocytic THP-1 cells onto cultured EC monolayer and EC-SMC co-culture. Journal of Zhejiang University. Science. B. 9:623-629.
Hamilton, R.T., L. Asatryan, J.T. Nilsen, J.M. Isas, T.K. Gallaher, T. Sawamura, and T.K. Hsiai. 2008. LDL protein nitration: Implication for LDL protein unfolding. Archives of biochemistry and biophysics. 479:1-14.
Hamilton, R.T.L. 2008. LDL protein nitration: Implication for protein unfolding and mitochondrial function by p-JNK-2. PhD thesis, UNIVERSITY OF SOUTHERN CALIFORNIA.
Igarashi, K., and M. Miura. 2008. Inhibition of a Radiation-Induced Senescence-Like Phenotype: A Possible Mechanism for Potentially Lethal Damage Repair in Vascular Endothelial Cells. Radiation Research. 170:534-539.
Mathews, D.T., Y.A. Birney, P.A. Cahill, and G.B. McGuinness. 2008. Vascular cell viability on polyvinyl alcohol hydrogels modified with water-soluble and -insoluble chitosan. Journal of biomedical materials research. Part B, Applied biomaterials. 84:531-540.
Mowbray, A.L. 2008. The role of peroxiredoxins as mechanosensitive antioxidants in endothelial cells. Georgia Institute of Technology, PhD dissertation.
Mowbray, A.L., D.-H. Kang, S.G. Rhee, S.W. Kang, and H. Jo. 2008. Laminar Shear Stress Up-regulates Peroxiredoxins (PRX) in Endothelial Cells: PRX 1 AS A MECHANOSENSITIVE ANTIOXIDANT. Journal of Biological Chemistry. 283:1622-1627.
Song, H. 2008. Endothelial bone morphogenic protein 4 and bone morphogenic protein receptor II expression in inflammation and atherosclerosis. Ph.D. thesis, GEORGIA INSTITUTE OF TECHNOLOGY.
Stoffels, E., A.J.M. Roks, and L.E. Deelman. 2008. Delayed Effects of Cold Atmospheric Plasma on Vascular Cells. Plasma Processes and Polymers. 5:599-605.
Wang, Y., and S. Lu. 2008. The application of FRET biosensors to visualize Src activation. In Proc. SPIE Vol. 6868. 68680A-68680A-68689.
2007
He, X., and T. Karino. 2007. Effects of a shear flow and water filtration on the cell layer of a hybrid vascular graft. Biomechanics at Micro-and Nanoscale Levels. 4:96-106.
Iantorno, M., H. Chen, J.-a. Kim, M. Tesauro, D. Lauro, C. Cardillo, and M.J. Quon. 2007. Ghrelin has novel vascular actions that mimic PI 3-kinase-dependent actions of insulin to stimulate production of NO from endothelial cells. American Journal of Physiology - Endocrinology and Metabolism. 292:E756-E764.
Kim, J.-a., G. Formoso, Y. Li, M.A. Potenza, F.L. Marasciulo, M. Montagnani, and M.J. Quon. 2007. Epigallocatechin Gallate, a Green Tea Polyphenol, Mediates NO-dependent Vasodilation Using Signaling Pathways in Vascular Endothelium Requiring Reactive Oxygen Species and Fyn. Journal of Biological Chemistry. 282:13736-13745.
Kishi, A., K. Yuasa, T. Matsukawa, T. Matsui, Y. Yamada, and I. Yamada. 2007. Agent for preventing arteriosclerosis. Patent Application US 20110009616 A1.
Klusacek, M., and V. Rizzo. 2007. ENDOTHELIAL CYTOSKELETAL REORGANIZATION IN RESPONSE TO PROTEASE ACTIVATED RECEPTOR-1 (PAR1) STIMULATION IS MEDIATED BY MEMBRANE RAFTS BUT NOT CAVEOLAE. Heart and Circulatory Physiology, March 16.
Kou, R., and T. Michel. 2007. Epinephrine Regulation of the Endothelial Nitric-oxide Synthase: ROLES OF RAC1 AND β3-ADRENERGIC RECEPTORS IN ENDOTHELIAL NO SIGNALING. Journal of Biological Chemistry. 282:32719-32729.
Ohashi, T., Y. Sugaya, N. Sakamoto, and M. Sato. 2007. Hydrostatic pressure influences morphology and expression of VE-cadherin of vascular endothelial cells. Journal of Biomechanics. 40:2399-2405.
Radel, C., M. Carlile-Klusacek, and V. Rizzo. 2007. Participation of caveolae in β1 integrin-mediated mechanotransduction. Biochemical and biophysical research communications. 358:626-631.
Sakai, T., K. Balasubramanian, S. Maiti, J.B. Halder, and A.J. Schroit. 2007. Plasmin-Cleaved β-2-Glycoprotein 1 Is an Inhibitor of Angiogenesis. The American journal of pathology. 171:1659-1669.
Yang, B., and V. Rizzo. 2007. TNF-α potentiates protein-tyrosine nitration through activation of NADPH oxidase and eNOS localized in membrane rafts and caveolae of bovine aortic endothelial cells. American Journal of Physiology-Heart and Circulatory Physiology. 292:H954-H962.
2006
DeMaio, L., M. Rouhanizadeh, S. Reddy, A. Sevanian, J. Hwang, and T.K. Hsiai. 2006. Oxidized phospholipids mediate occludin expression and phosphorylation in vascular endothelial cells. American Journal of Physiology - Heart and Circulatory Physiology. 290:H674-H683.
Formoso, G., H. Chen, J.-a. Kim, M. Montagnani, A. Consoli, and M.J. Quon. 2006. Dehydroepiandrosterone mimics acute actions of insulin to stimulate production of both nitric oxide and endothelin 1 via distinct phosphatidylinositol 3-kinase-and mitogen-activated protein kinase-dependent pathways in vascular endothelium. Molecular endocrinology. 20:1153-1163.
Liu, M.Y., M. Eyries, C. Zhang, F.S. Santiago, and L.M. Khachigian. 2006. Inducible platelet-derived growth factor D-chain expression by angiotensin II and hydrogen peroxide involves transcriptional regulation by Ets-1 and Sp1. Blood. 107:2322-2329.
Mathews, D.T. 2006. Characterisation of polyvinyl alcohol hydrogels modified with chitosan for cardiovascular applications. Dublin City University, PhD dissertation.
Mohri, S., J. Shimizu, N. Goda, T. Miyasaka, A. Fujita, M. Nakamura, and F. Kajiya. 2006. Measurements of CO2, lactic acid and sodium bicarbonate secreted by cultured cells using a flow-through type pH/CO2 sensor system based on ISFET. Sensors and Actuators B: Chemical. 115:519-525.
Niiya, Y., T. Abumiya, H. Shichinohe, S. Kuroda, S. Kikuchi, M. Ieko, S.-i. Yamagishi, M. Takeuchi, T. Sato, and Y. Iwasaki. 2006. Susceptibility of brain microvascular endothelial cells to advanced glycation end products-induced tissue factor upregulation is associated with intracellular reactive oxygen species. Brain research. 1108:179-187.
Ouedraogo, R., X. Wu, S.-Q. Xu, L. Fuchsel, H. Motoshima, K. Mahadev, K. Hough, R. Scalia, and B.J. Goldstein. 2006. Adiponectin Suppression of High-Glucose–Induced Reactive Oxygen Species in Vascular Endothelial Cells: Evidence for Involvement of a cAMP Signaling Pathway. Diabetes. 55:1840-1846.
Yang, B., T.N. Oo, and V. Rizzo. 2006. Lipid rafts mediate H2O2 prosurvival effects in cultured endothelial cells. The FASEB Journal. 20:1501-1503.
2005
Bonello, M., Y. Bobryshev, and L. Khachigian. 2005. Peroxide-Inducible Ets-1 Mediates Platelet-Derived Growth Factor Receptor-α Gene Transcription in Vascular Smooth Muscle Cells. Am J Pathol, 167:1149-1159.
Dilda, P.J., A.S. Don, K.M. Tanabe, V.J. Higgins, J.D. Allen, I.W. Dawes, and P.J. Hogg. 2005. Mechanism of Selectivity of an Angiogenesis Inhibitor From Screening a Genome-Wide Set of Saccharomyces cerevisiae Deletion Strains. Journal of the National Cancer Institute. 97:1539-1547.
Kieft, I., D. Darios, A. Roks and E. Stoffels. 2015. Plasma treatment of mammalian vascular cells: a quantitative description. Plasma Science, IEEE Transactions, 33:771-775.
Kou, R., S. SenBanerjee, M.K. Jain, and T. Michel. 2005. Differential Regulation of Vascular Endothelial Growth Factor Receptors (VEGFR) Revealed by RNA Interference: Interactions of VEGFR-1 and VEGFR-2 in Endothelial Cell Signaling†. Biochemistry. 44:15064-15073.
Potenza, M.A., F.L. Marasciulo, D.M. Chieppa, G.S. Brigiani, G. Formoso, M.J. Quon, and M. Montagnani. 2005. Insulin resistance in spontaneously hypertensive rats is associated with endothelial dysfunction characterized by imbalance between NO and ET-1 production. American Journal of Physiology - Heart and Circulatory Physiology. 289:H813-H822.
2004
Death, A.K., K.C. McGrath, M.A. Sader, S. Nakhla, W. Jessup, D.J. Handelsman, and D.S. Celermajer. 2004. Dihydrotestosterone promotes vascular cell adhesion molecule-1 expression in male human endothelial cells via a nuclear factor-κB-dependent pathway. Endocrinology. 145:1889-1897.
Motoshima, H., X. Wu, K. Mahadev, and B.J. Goldstein. 2004. Adiponectin suppresses proliferation and superoxide generation and enhances eNOS activity in endothelial cells treated with oxidized LDL. Biochemical and biophysical research communications. 315:264-271.
Niwa, K., T. Kado, J. Sakai, and T. Karino. 2004. The effects of a shear flow on the uptake of LDL and acetylated LDL by an EC monoculture and an EC–SMC coculture. Ann Biomed Eng. 32:537-543.
2003
Chen, H., M. Montagnani, T. Funahashi, I. Shimomura, and M.J. Quon. 2003. Adiponectin Stimulates Production of Nitric Oxide in Vascular Endothelial Cells. Journal of Biological Chemistry. 278:45021-45026.
2002
Khachigian, L. 2002. Treatment of cancer. Patent Application US 20030203864 A1.
2001
Gousseva, N., K. Kugathasan, C.N. Chesterman, and L.M. Khachigian. 2001. Early growth response factor-1 mediates insulin-inducible vascular endothelial cell proliferation and regrowth after injury*. Journal of cellular biochemistry. 81:523-534.
1999
Khachigian, L.M., F.S. Santiago, L.A. Rafty, O.L.-W. Chan, G.J. Delbridge, A. Bobik, T. Collins, and A.C. Johnson. 1999. GC Factor 2 Represses Platelet-Derived Growth Factor A-Chain Gene Transcription and Is Itself Induced by Arterial Injury. Circulation research. 84:1258-1267.
1997
Delbridge, G.J., and L.M. Khachigian. 1997. FGF-1–Induced Platelet-Derived Growth Factor-A Chain Gene Expression in Endothelial Cells Involves Transcriptional Activation by Early Growth Response Factor-1. Circulation research. 81:282-288.