MSDS Cryopreserved Cells
Cell Apps Flyer Smooth Muscle Cells
Cell Apps Flyer Cardiovascular Cells
5 Important Cell Culture Rules
Cell Apps Poster Primary Cells
Cell Applications Inc Brochure
Bovine Aortic Smooth Muscle Cells (BAOSMC) provide an excellent model system to study all aspects of cardiovascular function and disease, especially those related to mechanisms of hyperplasia and hypertrophy of intimal smooth muscle cells leading to vascular occlusion in atherosclerosis and stent restenosis.
BAOSMC from Cell Applications, Inc. have been utilized in a number of research studies, for example, to:
- Elucidate RhoA-dependent serum response signaling pathways
- Observe the role of FAK in thrombospondin-1 induced migration of vascular smooth muscle cells
- Demonstrate the relationship between the disturbed flow and proliferation of smooth muscle cells, leading to intima hyperplasia
- Study interactions between lipoproteins and extracellular matrix, as well as mechanisms of lipoprotein aggregation contributing to atherosclerosis
- Construct an expression cassette to maximize targeted transgene expression
- Test new technologies, such as on-chip contact imaging technique, handheld fluorometers and a “nose on a chip” olfactory sensor
- Study the behavior of smooth muscle cells in 3d environment and smooth muscle/endothelial co-cultures
- Describe an origami-like method of generating three-dimensional (3D) cell-laden microstructures
- Design materials for tissue engineering and cardiovascular implants
Characterization: positive for smooth muscle cell specific alpha-actin expression
|Normal healthy bovine aorta|
No bacteria, yeast, fungi, mycoplasma
Smooth muscle specific α-actin positive
|Attach, spread, proliferate in Growth Med|
500,000 BAOSMC (2nd passage) frozen in Basal Medium w/ 10% FBS, 10% DMSO
Cryovial frozen BAOSMC(B354-05), Growth Medium (311-500), Subcltr Rgnt Kit (090K)
Shipped in Gr Med, 3rd psg (flasks or plates)
At least 16
Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
|Cryopreserved BAOSMC: 5x10^5 Cells, Medium & Subculture Reagents (See Details tab for specifics)||Size: 1 Kit||CAT.#: B354K-05||Price: $769.00|
|Cryopreserved BAOSMC: Frozen BAOSMC (5x10^5)||Size: 1 Cryovial||CAT.#: B354-05||Price: $619.00|
|Proliferating BAOSMC: Actively growing, dividing cells in medium||Size: T-25 Flask||CAT.#: B355-25||Price: $619.00|
|Proliferating BAOSMC: Actively growing, dividing cells in medium||Size: T-75 Flask||CAT.#: B355-75||Price: $823.00|
|Proliferating BAOSMC: Actively growing, dividing cells in medium||Size: 6 Well||CAT.#: 355-6W||Price: $665.00|
|Proliferating BAOSMC: Actively growing, dividing cells in medium||Size: 96 Well||CAT.#: B355-96W||Price: $952.00|
|Bovine SMC Basal Medium: Basal medium (contains no growth supplement). Add GS before use.||Size: 500 ml||CAT.#: B310-500||Price: $57.00|
|Bovine SMC Growth Medium: All-in-one ready-to-use||Size: 500 ml||CAT.#: B311-500||Price: $105.00|
|Bovine SMC Growth Medium Kit: Basal medium & growth supplement sold together packaged separately||Size: Yields 500 ml||CAT.#: B311K-500||Price: $113.00|
|Bovine SMC Growth Supplement: Added to Basal Medium to create Growth Medium||Size: 50 ml (2 parts)||CAT.#: B311-GS||Price: $57.00|
|Bovine SMC Induction Medium: Provides signals that change cell behavior||Size: 250 ml||CAT.#: B311I-250||Price: $62.00|
Extended Family Products
|Cyto-X Cell Counting Reagent: 500 tests||Size: 1 Bottle||CAT.#: 028-01||Price: $139.00|
|Cyto-X Cell Counting Reagent Sample: 100 tests||Size: Sample||CAT.#: 028-S||Price: $36.00|
|Cytofect™ Smooth Muscle Cell Transfection Kit: 125 x 24-Well||Size: 1 Kit||CAT.#: TF350K||Price: $285.00|
|Cytofect™ Smooth Muscle Cell Transfection Kit: 25 x 24-Well Rxns||Size: 1 Sample Kit||CAT.#: TF350KS||Price: $54.00|
|Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO.||Size: 50 ml||CAT.#: 040-50||Price: $54.00|
|Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution||Size: 1 Kit||CAT.#: 090K||Price: $55.00|
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Fan, L., J. Sakai, S. Bessho, S. Wada, and T. Karino. 2010. Effect of a disturbed flow on adhesion of monocytes to a model of an arterial wall. Biorheology. 47:15-29.
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Fan, L.J., and T. Karino. 2008. Effect of serum concentration on adhesion of monocytic THP-1 cells onto cultured EC monolayer and EC-SMC co-culture. Journal of Zhejiang University. Science. B. 9:623-629.
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Wang, X.-J., K. Maier, S. Fuse, A.I. Willis, E. Olson, S. Nesselroth, B.E. Sumpio, and V. Gahtan. 2008. Thrombospondin-1-induced migration is functionally dependent upon focal adhesion kinase. Vascular and endovascular surgery. 42:256-262.
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