MSDS Cryopreserved Cells
Instructions BCAEC
5 Important Cell Culture Rules
Cell Apps Fllyer Cardiovascular Cells
Cell Apps Flyer Endothelial Cells
Cell Apps Poster Primary Cells
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Description
Bovine Coronary Artery Endothelial Cells (BCAEC) from CAI have been used to demonstrate that stress hormone cortisol inhibits NO synthase and Ca2+ mobilization, leading to decreased NO release by endothelial cells, resulting in increased arterial contraction and decreased blood flow. A Similar action mechanism was determined for cocaine effects on arteries. Additionally, it was found that cocaine-induced apoptosis of endothelial cells is mediated by attenuation of NO production, down-regulation of Bcl-2 and translocation of Bac to the mitochondria, followed by release of cytochrome c and activation of caspase 9 and caspase 3. BCAEC were also used to determine diabetes-related vascular dysfunction. Others employed the cells to evaluate novel mesh-like materials for their suitability for biomedical applications.
(Click to Enlarge) Bovine Coronary Artery Endothelial Cells: BCAEC. Isolated from blood vessel tissue.
Details
Tissue | Normal healthy bovine coronary artery |
QC | No bacteria, yeast, fungi, mycoplasma |
Character | DiI-Ac-LDL uptake: Positive |
Bioassay | Attach, spread, proliferate in Growth Med |
Cryovial | 500,000 BCAEC (2nd passage) frozen in Basal Medium w/ 10% FBS & 10% DMSO |
Kit | Cryovial frozen BCAEC (B300-05), Growth Med (B211-500), Subcltr Rgnt Kit (090K) |
Proliferating | Shipped in Gr Med, 4th psg (flasks or plates) |
Doublings | At least 16 |
Applications | Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use. |
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Cryopreserved Bovine Coronary Artery Endothelial Cells Total Kit: 5x10^5 Cells, Medium & Subculture Reagents (See Details tab for specifics) | Size: 1 Kit | CAT.#: B300K-05 | Price: $869.00 | |
| Cryopreserved Bovine Coronary Artery Endothelial Cells (BCAEC): Frozen BCAEC (5x10^5) | Size: 1 Cryovial | CAT.#: B300-05 | Price: $695.00 | |
| Proliferating Bovine Coronary Artery Endothelial Cells (BCAEC): Actively growing, dividing cells in medium | Size: T-25 Flask | CAT.#: B301-25 | Price: $695.00 | |
| Proliferating Bovine Coronary Artery Endothelial Cells (BCAEC): Actively growing, dividing cells in medium | Size: T-75 Flask | CAT.#: B301-75 | Price: $885.00 | |
| Proliferating Bovine Coronary Artery Endothelial Cells (BCAEC): Actively growing, dividing cells in medium | Size: 24 Well | CAT.#: B301-24W | Price: $885.00 | |
| Proliferating Bovine Coronary Artery Endothelial Cells (BCAEC): Actively growing, dividing cells in medium | Size: 96 Well | CAT.#: B301-96W | Price: $1,005.00 |
Related Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Bovine EC Basal Medium : Basal medium (contains no growth supplement). Add GS before use. | Size: 500 ml | CAT.#: B210-500 | Price: $74.00 | |
| Bovine EC Growth Medium: All-in-one ready-to-use | Size: 500 ml | CAT.#: B211-500 | Price: $121.00 | |
| Bovine EC Growth Medium Kit: Basal medium & growth supplement sold together packaged separately | Size: Yields 500 ml | CAT.#: B211K-500 | Price: $130.00 | |
| Bovine EC Growth Supplement: Added to Basal Medium to create Growth Medium | Size: 30 ml | CAT.#: B211-GS | Price: $66.00 |
Extended Family Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO. | Size: 50 ml | CAT.#: 040-50 | Price: $54.00 | |
| Cytofect Endothelial Cell Transfection Kit (250 x 24-Wells): 250 x 24-Well Rxns | Size: 1 Kit | CAT.#: TF101K | Price: $496.00 | |
| Cytofect Endothelial Cell Transfection Sample Kit (25 x 24-Wells): 25 x 24-Well Rxns | Size: 1 Sample Kit | CAT.#: TF101KS | Price: $62.00 | |
| Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution | Size: 1 Kit | CAT.#: 090K | Price: $63.00 |
Resources/Documents
Publications
2017
Beltrán-Partida, E., B. Valdéz-Salas, A. Moreno-Ulloa, A. Escamilla, M. Curiel, R. Rosales-Ibáñez, F. Villarreal, D. Bastidas and J. Bastidas. 2017. Improved in vitro angiogenic behavior on anodized titanium dioxide nanotubes. J Nanobiotech, 15:10.
Hara, T., T. Kojima, H. Matsuzaki, T. Nakamura, E., Yoshida, Y. Fujiwara, C. Yamomoto, S. Saito and T. Kaji. 2017. Induction of Syndecan-4 by Organic–Inorganic Hybrid Molecules with a 1,10-Phenanthroline Structure in Cultured Vascular Endothelial Cells. Int J Mol Sci, 18:352.
2015
Moreno-Ulload, A., D. Mendez-Luna, E. Beltran-Partida, C. Castillo, G. Guevara, I. Ramirez-Sanchez, J. Correa-Basurto, G. Ceballos, and F. Villarreal. 2015. The effects of (−)-epicatechin on endothelial cells involve the G protein-coupled estrogen receptor (GPER). Pharmacological Research, doi:10.1016/j.phrs.2015.08.014.
Takizawa, Y., R. Nakata, K. Fukuhara, H. Yamashita, H. Kubodera and H. Inoue. 2015. The 4-Hydroxyl Group of Resveratrol Is Functionally Important for Direct Activation of PPARa. PLoS ONE 10(3): e0120865.
Varela, C., E. Fromentin, M. Roller, F. Villarreal, and I. Ramirez-Sanchez. 2015. Effects of a Natural Extract of Aronia melanocarpa Berry on Endothelial Cell Nitric Oxide Production. J Food Biochem, doi: 10.1111/jfbc.12226.
2008
Romero, M.J., D.H. Platt, H.E. Tawfik, M. Labazi, A.B. El-Remessy, M. Bartoli, R.B. Caldwell, and R.W. Caldwell. 2008. Diabetes-induced Coronary Vascular Dysfunction Involves Increased Arginase Activity. Circulation research. 102:95-102.
2007
Niwa, K., J. Sakai, T. Watanabe, T. Ohyama, and T. Karino. 2007. Improved arterial wall model by coculturing vascular endothelial and smooth muscle cells. In vitro cellular & developmental biology. Animal. 43:17-20.
2005
He, J., S. Yang, and L. Zhang. 2005. Effects of Cocaine on Nitric Oxide Production in Bovine Coronary Artery Endothelial Cells. Journal of Pharmacology and Experimental Therapeutics. 314:980-986.
2004
Nair, L.S., S. Bhattacharyya, J.D. Bender, Y.E. Greish, P.W. Brown, H.R. Allcock, and C.T. Laurencin. 2004. Fabrication and Optimization of Methylphenoxy Substituted Polyphosphazene Nanofibers for Biomedical Applications. Biomacromolecules. 5:2212-2220.
2002
Rogers, K.M., C.A. Bonar, J.L. Estrella, and S. Yang. 2002. Inhibitory effect of glucocorticoid on coronary artery endothelial function. American Journal of Physiology - Heart and Circulatory Physiology. 283:H1922-H1928.
2001
He, J., Y. Xiao, and L. Zhang. 2001. Cocaine-Mediated Apoptosis in Bovine Coronary Artery Endothelial Cells: Role of Nitric Oxide. Journal of Pharmacology and Experimental Therapeutics. 298:180-187.
2000
He, J., Y. Xiao, C.A. Casiano, and L. Zhang. 2000. Role of Mitochondrial Cytochrome c in Cocaine-Induced Apoptosis in Coronary Artery Endothelial Cells. Journal of Pharmacology and Experimental Therapeutics. 295:896-903.