MSDS Cryopreserved Cells
Instructions BCAEC
5 Important Cell Culture Rules
Cell Apps Fllyer Cardiovascular Cells
Cell Apps Flyer Endothelial Cells
Cell Apps Poster Primary Cells
Cell Applications Inc Brochure

Description

Bovine Coronary Artery Endothelial Cells (BCAEC) from CAI have been used to demonstrate that stress hormone cortisol inhibits NO synthase and Ca2+ mobilization, leading to decreased NO release by endothelial cells, resulting in increased arterial contraction and decreased blood flow.  A Similar action mechanism was determined for cocaine effects on arteries.  Additionally, it was found that cocaine-induced apoptosis of endothelial cells is mediated by attenuation of NO production, down-regulation of Bcl-2 and translocation of Bac to the mitochondria, followed by release of cytochrome c and activation of caspase 9 and caspase 3.  BCAEC were also used to determine diabetes-related vascular dysfunction. Others employed the cells to evaluate novel mesh-like materials for their suitability for biomedical applications.
(Click to Enlarge) Bovine Coronary Artery Endothelial Cells: BCAEC.  Isolated from blood vessel tissue.

Details

Tissue
Normal healthy bovine coronary artery
QC
No bacteria, yeast, fungi, mycoplasma
Character
DiI-Ac-LDL uptake: Positive
Bioassay
Attach, spread, proliferate in Growth Med
Cryovial
500,000 BCAEC (2nd passage) frozen in Basal Medium w/ 10% FBS & 10% DMSO
Kit
Cryovial frozen BCAEC (B300-05), Growth Med (B211-500), Subcltr Rgnt Kit (090K)
Proliferating
Shipped in Gr Med, 4th psg (flasks or plates)
Doublings
At least 16
Applications
Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
Instructions BCAEC

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MSDS Cryopreserved Cells

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Products

Product Size CAT.# Price Quantity
Cryopreserved BCAEC: Frozen BCAEC (5x10^5) Size: 1 Cryovial CAT.#: B300-05 Price: $586.56
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Cryopreserved BCAEC Total Kit: 5x10^5 Cells, Medium & Subculture Reagents (See Details tab for specifics) Size: 1 Kit CAT.#: B300K-05 Price: $735.59
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Proliferating BCAEC: Actively growing, dividing cells in medium Size: T-25 Flask CAT.#: B301-25 Price: $586.56
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Proliferating BCAEC: Actively growing, dividing cells in medium Size: T-75 Flask CAT.#: B301-75 Price: $791.04
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Proliferating BCAEC: Actively growing, dividing cells in medium Size: 6 Well CAT.#: B301-6W Price: $737.23
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Proliferating BCAEC: Actively growing, dividing cells in medium Size: 96 Well CAT.#: B301-96W Price: $866.38
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Related Products

Product Size CAT.# Price Quantity
Bovine Endothelial Cell Basal Medium: Basal medium (contains no growth supplement).  Add GS before use. Size: 500 ml CAT.#: B210-500 Price: $57.04
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Bovine Endothelial Cell Growth Medium: All-in-one ready-to-use Size: 500 ml CAT.#: B211-500 Price: $105.47
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Bovine Endothelial Cell Growth Medium Kit: Basal medium & growth supplement sold together packaged separately Size: Yields 500 ml CAT.#: B211K-500 Price: $113.01
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Bovine Endothelial Cell Growth Supplement: Added to Basal Medium to create Growth Medium Size: 30 ml CAT.#: B211-GS Price: $50.58
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Extended Family Products

Product Size CAT.# Price Quantity
Cell Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO. Size: 50 ml CAT.#: 040-50 Price: $53.81
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Cyto-X Colorimetric Cell Counting Reagent: 500 tests Size: 1 Bottle CAT.#: 028-01 Price: $138.84
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Cyto-X Colorimetric Cell Counting Reagent: 100 tests Size: Sample CAT.#: 028-S Price: $35.88
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Cytofect™ Endothelial Cell Transfection Kit: 250 x 24-Well Rxns Size: 1 Kit CAT.#: TF101K Price: $430.50
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Cytofect™ Endothelial Cell Transfection Kit: 25 x 24-Well Rxns Size: 1 Sample Kit CAT.#: TF101KS Price: $53.76
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Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution Size: 1 Kit CAT.#: 090K Price: $54.89
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Resources/Documents

5 Important Cell Culture Rules

Format: PDF

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Cell Apps Flyer Endothelial Cells

Format: PDF

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Cell Apps Poster Primary Cells

Format: PDF

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Cell Applications Inc Brochure

Format: PDF

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Publications

2017
Beltrán-Partida, E., B. Valdéz-Salas, A. Moreno-Ulloa, A. Escamilla, M. Curiel, R. Rosales-Ibáñez, F. Villarreal, D. Bastidas and J. Bastidas. 2017Improved in vitro angiogenic behavior on anodized titanium dioxide nanotubes. J Nanobiotech, 15:10.
Hara, T., T. Kojima, H. Matsuzaki, T. Nakamura, E., Yoshida, Y. Fujiwara, C. Yamomoto, S. Saito and T. Kaji. 2017. Induction of Syndecan-4 by Organic–Inorganic Hybrid Molecules with a 1,10-Phenanthroline Structure in Cultured Vascular Endothelial Cells. Int J Mol Sci, 18:352.
2015
Moreno-Ulload, A., D. Mendez-Luna, E. Beltran-Partida, C. Castillo, G. Guevara, I. Ramirez-Sanchez, J. Correa-Basurto, G. Ceballos, and F. Villarreal. 2015. The effects of (−)-epicatechin on endothelial cells involve the G protein-coupled estrogen receptor (GPER). Pharmacological Research, doi:10.1016/j.phrs.2015.08.014.
Takizawa, Y., R. Nakata, K. Fukuhara, H. Yamashita, H. Kubodera and H. Inoue. 2015. The 4-Hydroxyl Group of Resveratrol Is Functionally Important for Direct Activation of PPARa. PLoS ONE 10(3): e0120865.
Varela, C., E. Fromentin, M. Roller, F. Villarreal, and I. Ramirez-Sanchez. 2015. Effects of a Natural Extract of Aronia melanocarpa Berry on Endothelial Cell Nitric Oxide Production. J Food Biochem, doi: 10.1111/jfbc.12226.
2008
Romero, M.J., D.H. Platt, H.E. Tawfik, M. Labazi, A.B. El-Remessy, M. Bartoli, R.B. Caldwell, and R.W. Caldwell. 2008. Diabetes-induced Coronary Vascular Dysfunction Involves Increased Arginase Activity. Circulation research. 102:95-102.
2007
Niwa, K., J. Sakai, T. Watanabe, T. Ohyama, and T. Karino. 2007. Improved arterial wall model by coculturing vascular endothelial and smooth muscle cells. In vitro cellular & developmental biology. Animal. 43:17-20.
2005
He, J., S. Yang, and L. Zhang. 2005. Effects of Cocaine on Nitric Oxide Production in Bovine Coronary Artery Endothelial Cells. Journal of Pharmacology and Experimental Therapeutics. 314:980-986.
2004
Nair, L.S., S. Bhattacharyya, J.D. Bender, Y.E. Greish, P.W. Brown, H.R. Allcock, and C.T. Laurencin. 2004. Fabrication and Optimization of Methylphenoxy Substituted Polyphosphazene Nanofibers for Biomedical Applications. Biomacromolecules. 5:2212-2220.
2002
Rogers, K.M., C.A. Bonar, J.L. Estrella, and S. Yang. 2002. Inhibitory effect of glucocorticoid on coronary artery endothelial function. American Journal of Physiology - Heart and Circulatory Physiology. 283:H1922-H1928.
2001
He, J., Y. Xiao, and L. Zhang. 2001. Cocaine-Mediated Apoptosis in Bovine Coronary Artery Endothelial Cells: Role of Nitric Oxide. Journal of Pharmacology and Experimental Therapeutics. 298:180-187.
2000
He, J., Y. Xiao, C.A. Casiano, and L. Zhang. 2000. Role of Mitochondrial Cytochrome c in Cocaine-Induced Apoptosis in Coronary Artery Endothelial Cells. Journal of Pharmacology and Experimental Therapeutics. 295:896-903.