MSDS Cryopreserved Cells
Instructions BEnEpC
Cell Apps Flyer Epithelial Cells
5 Important Cell Culture Rules
Cell Apps Poster Primary Cells
Cell Applications Inc Brochure

Description

Bovine Endometrial Epithelial Cells (BEnEpC) from Cell Applications, Inc. provide an excellent model system to study many aspects of epithelial function and disease, especially those related to the endometrium and pregnancy-related signaling, such as seminal plasma induced prostaglandins F2α, (PGF2α), E2 (PGE2) and interleukin-6 release by endometrial cells, or the role of PLA in mediating oxytocin-stimulated production of prostaglandins.  BEnEpC have also been used to demonstrate the association between variants of gap junctions related genes and conception rate, and to investigate cellular adherence and cytotoxicity of Histophilus somni.
(Click to Enlarge) Bovine Endometrial Epithelial Cells: BEnEpC.  Isolated from the inner membrane of the mammalian uterus.

Details

Tissue
Normal healthy endometrial epithelium
QC
No bacteria, yeast, fungi, mycoplasma
Bioassay
Attach, spread, proliferate in Growth Med
Cryovail
500,000 BEnEpC (2nd passage) frozen in Basal Medium w/ 10% FBS, 10% DMSO
Kit
Cryovial frozen BEnEpC (B932-05), Growth Medium (B911-500), Subcltr Rgnt Kit (090K)
Proliferating
Shipped in Gr Med, 3rd psg (flasks or plates)
Doublings
At least 10
Instructions BEnEpC

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MSDS Cryopreserved Cells

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Products

Product Size CAT.# Price Quantity
Cryopreserved BEnEpC: Frozen BEnEpC (5x10^5) Size: 1 Cryovial CAT.#: B932-05 Price: $726.47
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Cryopreserved BEnEpC Total Kit: 5x10^5 Cells, Medium & Subculture Reagents (See Details tab for specifics) Size: 1 Kit CAT.#: B932K-05 Price: $872.00
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Proliferating BEnEpC: Actively growing, dividing cells in medium Size: T-25 Flask CAT.#: B933-25 Price: $726.47
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Proliferating BEnEpC: Actively growing, dividing cells in medium Size: T-75 Flask CAT.#: B933-75 Price: $930.96
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Proliferating BEnEpC: Actively growing, dividing cells in medium Size: 6 Well CAT.#: B933-6W Price: $877.14
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Proliferating BEnEpC: Actively growing, dividing cells in medium Size: 96 Well CAT.#: B933-96W Price: $1,006.29
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Related Products

Product Size CAT.# Price Quantity
Bovine Endometrial Cell Basal Medium: Basal medium (contains no growth supplement).  Add GS before use. Size: 500 ml CAT.#: B910-500 Price: $57.04
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Bovine Endometrial Cell Growth Medium: All-in-one ready-to-use Size: 500 ml CAT.#: B911-500 Price: $105.47
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Bovine Endometrial Cell Growth Medium Kit: Basal medium & growth supplement sold together packaged separately Size: Yields 500ml CAT.#: B911K-500 Price: $113.01
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Bovine Endometrial Cell Growth Supplement: Added to Basal Medium to create Growth Medium Size: 50 ml x 2 CAT.#: B911-GS Price: $57.04
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Extended Family Products

Product Size CAT.# Price Quantity
Cell Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO. Size: 50 ml CAT.#: 040-50 Price: $53.81
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Cyto-X Colorimetric Cell Counting Reagent: 500 tests Size: 1 Bottle CAT.#: 028-01 Price: $138.84
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Cyto-X Colorimetric Cell Counting Reagent: 100 tests Size: Sample CAT.#: 028-S Price: $35.88
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Cytofect™ Epithelial Cell Transfection Kit: 124 x 24-Well Rxns Size: 1 Kit CAT.#: TF102K Price: $403.59
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Cytofect™ Epithelial Cell Transfection Kit: 25 x 24-Well Rxns Size: 1 Sample Kit CAT.#: TF102KS Price: $53.76
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Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution Size: 1 Kit CAT.#: 090K Price: $54.89
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Resources/Documents

Cell Apps Flyer Epithelial Cells

Format: PDF

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5 Important Cell Culture Rules

Format: PDF

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Cell Apps Poster Primary Cells

Format: PDF

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Cell Applications Inc Brochure

Format: PDF

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Publications

2016
Brockus, K., C. Hart, C. Gilfeather, B. Fleming, and C. Lemley. 2016. Dietary melatonin alters uterine artery hemodynamics in pregnant Holstein heifers. Domestic Animal Endocrinology, 55:1-10.
Gilfeather, C. and C. Lemley. 2016. Effects of Interferon-Tau and Steroids on Cytochrome P450 Activity in Bovine Endometrial Epithelial Cells. Reproduction in Domestic Animals, 51:415-420.
Sugimoto, M., Y. Gotoh, T. Kawahara and Y. Sugimoto. 2016. Family with Sequence Similarity 213, Member A is Associated with the Fertility Selection Index in Holsteins. J Fertility Miomarkers, Vol-1 Issue 1 Pg. no.- 20.
2013
Madej, M., C. Hansen, A. Johannisson, and A. Madej. 2013. Heparin-binding proteins from boar seminal plasma affecting the release of prostaglandins and interleukin-6 by porcine endometrial and cervical cells and bovine endometrial cells. Science Research (Natural Science), 5:21-30.
Malgorzata, M., H. Claus, J. Anders, and M. Andrzej. 2013. Heparin-binding proteins from boar seminal plasma affecting the release of prostaglandins and interleukin-6 by porcine endometrial and cervical cells and bovine endometrial cells. Natural Science. 5:21.
Sugimoto, M., S. Sasaki, Y. Gotoh, Y. Nakamura, Y. Aoyagi, T. Kawahara, and Y. Sugimoto. 2013. Genetic variants related to gap junctions and hormone secretion influence conception rates in cows. Proceedings of the National Academy of Sciences. 10.1073/pnas.1309307110
2012
Madej, M., M. Norrby, M.T. Madsen, A. Johannisson, C. Hansen, and A. Madej. 2012. The Effect of Boar Seminal Plasma on the Release of Prostaglandins and Interleukin-6 by Porcine Endometrial and Cervical Cells and Bovine Endometrial Cells. Reproduction in Domestic Animals. 47:113-124.
2010
Norrby, M. 2010. Hormones and sex steroid receptors in the female pig. Vol. 2010. Swedish University of Agricultural Sciences, PhD dissertation.
2009
Hoshinoo, K., K. Sasaki, A. Tanaka, L.B. Corbeil, and Y. Tagawa. 2009. Virulence attributes of Histophilus somni with a deletion mutation in the ibpA gene. Microbial Pathogenesis. 46:273-282.
Krishnaswamy, N., G. Danyod, P. Chapdelaine, and M.A. Fortier. 2009. Oxytocin Receptor Down-Regulation Is Not Necessary for Reducing Oxytocin-Induced Prostaglandin F2α Accumulation by Interferon-τ in a Bovine Endometrial Epithelial Cell Line. Endocrinology. 150:897-905.
2007
Tithof, P.K., M.P. Roberts, W. Guan, M. Elgayyar, and J.D. Godkin. 2007. Distinct phospholipase A2 enzymes regulate prostaglandin E2 and F2alpha production by bovine endometrial epithelial cells. Reproductive Biology and Endocrinology. 5:16.